...many organisms. When supplemented with adenine analogs such as 2-fluoroadenine (2-FA), organisms with ...Zurich. He is fascinated by how organisms exquisitely and robustly self-organize at the cellular and multicellular...Plant Cell 25:1507–1522 . https://doi.org/10.1105/tpc.113.112235 Morgens DW, Deans RM, Li A, Bassik MC (...Fortunately, researchers studying plants and other organisms lacking this innate immunity need not worry and...different genes known to be required for viability (Morgens et al., 2016). Only upon combining the data generated...plants Once you’ve introduced your RNAi into your organism, how do you identify the actively silenced ones... of APT and any gene target of interest, only organisms that are actively silencing to a sufficient degree...

... Ref BphP-based FPs miRFP670 https://www.addgene.org/79987/ 642 670 87,400 14.0 198 Monomer  155...155 4.5 72 5 miRFP703 https://www.addgene.org/79988/ 674 703 90,900 8.6 127 Monomer 394 4.5 37 ... miRFP709 https://www.addgene.org/79989/ 683 709 78,400 5.4 69 Monomer 192 4.5 30 mIFP https://www.addgene.org...112 Monomer 54 4.5 15  15 miRFP https://www.addgene.org/108409/ 674 703 92,400 9.7 145 Monomer ND ...ND 4.3 ND 16 IFP2.0 e https://www.addgene.org/54785/ 688 709 98,000 8.1 128 Dimer 108 4.5 8  17 ... iRFP670 https://www.addgene.org/45457/ 643 670 114,000 12.2 225 Dimer 290 4.0 119 18 iRFP682 https...https://www.addgene.org/45459/ 663 682 90,000 11.1 162 Dimer 490 4.5 105 iRFP702 https://www.addgene.org...

...reagents to use - organic solvents and detergents.  Organic Solvents  As mentioned above, organic solvents can... for use with this type of fixation*. Organic Solvents Organic solvents, generally acetone, methanol,...types of fixatives - chemical cross-linkers and organic solvents.  Chemical Cross-Linkers Chemical cross-linkers...quite as well as a cross-linker. This is because organic solvents can be quite harsh - lipids and soluble...disrupt your target epitope (Fig. 1). So, while organic solvents may improve antibody binding for some ...targets, they can destroy it for others. That said, organic solvents’ impact on lipids means that they are ...and permeabilize a sample. But you can also use organic solvents after fixing your sample with a cross-...

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...and biomedical scientists (see: bioRxiv.org or medRxiv.org) in the past few years.   Fig. 1: Stand...fields like math and physics for decades (see: arXiv.org), but have been only adopted by biological and...their CV or grant applications. A number of organizations (such as ASAPbio) advocate for preprints, and...manuscript to a journal for formal peer review. Some organizations, including some journals, also support open ...general public (say, policymakers or community organizations) to use your work but don’t think they’ll have...here at Addgene! Along with other open-science organizations, we support scientists in sharing their results...out there and finish that experiment (and don’t forget the controls!). And when you’re ready to write ...

...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...CRISPR–Cas9 screens. Nature 568:511–516 . https://doi.org/10.1038/s41586-019-1103-9 Bialek JK, Dunay GA, ...Activator Systems. PLoS ONE 11:e0158294 . https://doi.org/10.1371/journal.pone.0158294 Chari R, Mali P, Moosburner...library-on-library approach. Nat Methods 12:823–826 . https://doi.org/10.1038/nmeth.3473 Chavez A, Scheiman J, Vora S... programming. Nat Methods 12:326–328 . https://doi.org/10.1038/nmeth.3312 Chavez A, Tuttle M, Pruitt BW...multiple species. Nat Methods 13:563–567 . https://doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck MA, Adamson...

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... a variety of functions that allow their host organisms to thrive in competitive environments. The genes...the competition, and Penicillium isn’t the only organism that knows it. Some bacteria possess plasmid-borne...encode genes that allow the host to infect other organisms. If you’ve ever wondered why E. coli is safe to...means to deliver DNA and other molecules to host organisms. For instance, vectors derived from the Agrobacterium...like by living where no one else wants to. Some organisms manage this with plasmids that allow them to eat... eat unusual substrates that other organisms can’t use. Other plasmids offer resistance to toxic substances... which protect the host from attacks by other organisms. To conserve energy, these specialized degradative...

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