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We narrowed to 658 results for: otos

Showing: 161 - 180 of 658 results

  1. Zhang Lab CRISPR Page

    Type
    Collection
    ...Nature Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 ...amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols 2013). SpCas9 Cas9...Website You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9...co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual human codon-optimized SpCas9...insertion of guide sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing...cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors for...PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc . 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143...

  2. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...therapy. The laws behind phage therapy, acceptable endotoxin levels, etc. can vary depending where treatment...information on individual phages. Integrating lab protocols, clinical information, phage research job postings...resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers page ...

  3. Zinc Finger Consortium: OPEN Reagents

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    Collection
    ...Resources A detailed protocol for practicing OPEN is available in the 2009 Joung Nature Protocols paper . How ... Scot Wolfe Lab plasmids Detailed Information Protocols & Resources Contents of Kit Original Publication...Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder...Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols . 2009;4(10):1471-501. doi: 10.1038/nprot.2009.98...

  5. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ... involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...

  8. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post
    ...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...the CRISPR/CAS toolkit by posting generic lab protocols, providing tips from experts in the field, and... A. J Bacteriol. 1987 Dec; 169 (12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James...

  9. Antibody Production

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    Collection
    ...For more details on protocols see the Addgene Protocols page or the Neuromab Protocols (Link opens in a ...are initially titered by spectrophotometry using a NanoDrop spectrophotometer. If needed, the sample is...before distribution. Details about our production protocols, titering methods, and quality control are described...Recipient Instructions Addgene Help Center Antibody Protocols...

  12. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...including a link to a detailed CellTag protocol on protocols.io, code and analysis tutorials on GitHub...Hnf4α, a process that like other reprogramming protocols is inefficient. Cells were sequentially tagged...

  13. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...brightest teal fluorescent proteins that were photostable and had a high 480/530 nm emission ratio. They...mWasabi is 1.6-fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks...Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein...

  14. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...10 kb) or has a low copy number, try following protocols for low copy plasmids. For example, using a longer... intact plasmid. Please see our gel extraction protocol for more details.   Figure 2: An uncut... Find more molecular biology and viral vector protocols Learn about the common lab E. coli strains Find...

  16. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

  17. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...concentrating your virus. See our Concentrating AAV Protocol (Step 10) for instructions on how to do this. ...take below but you should read our AAV Production Protocol for additional best practices. Keep your cells...Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols for Work with Virus Watch Our AAV Purification...

  18. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...lentiviruses easily made in your own lab. We provide protocols and useful tips for generating your own lentivirus...using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...lentiviruses challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast...

  19. Clear and Accessible Writing

    Type
    Blog Post
    ... from it? The image in Figure 1 appears on our protocol page for AAV Purification by Iodixanol Gradient...post.  Here, we are presenting this image on a protocol page on our website, as an instructive step-by-step...chose this alt text: "The left panel is a profile photo of an iodixanol gradient after ultracentrifugation...

  20. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions...mammalian cells using 293T cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.17C. doi: ...Research More resources on addgene.org Addgene Protocols: General Transfection The Addgene Retrovirus Guide...
Showing: 161 - 180 of 658 results