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...These kits provide in-depth, ready-to-teach lab protocols and supporting materials such as instructional...conditions, to guiding students through specific protocols, ABRC staff are available via e-mail and phone...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants Parts Kit...

...including a link to a detailed CellTag protocol on protocols.io, code and analysis tutorials on GitHub...Hnf4α, a process that like other reprogramming protocols is inefficient. Cells were sequentially tagged...

...brightest teal fluorescent proteins that were photostable and had a high 480/530 nm emission ratio. They...mWasabi is 1.6-fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks...Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein...

...10 kb) or has a low copy number, try following protocols for low copy plasmids. For example, using a longer... intact plasmid. Please see our gel extraction protocol for more details.   Figure 2: An uncut... Find more molecular biology and viral vector protocols Learn about the common lab E. coli strains Find...

...steady stream of papers describing new photosynthetic or photosensory molecules that come from basically ...would be helpful to have molecules with better two-photon properties, so you could activate single cells ...

... Lab TAP Tagging Protocol 97.2 KB Yamamoto PITCh Tagging System The Takashi Yamamoto lab has deposited...provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB Mendenhall...can be found in Sakuma et al., Nature Protocols, 2016 . Yamamoto Tagging Plasmids: pX330S-2-PITCh - expresses...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR...tagging techniques, as well as the plasmids and protocols needed for using them, are described below. Read...2014 The Förstemann lab has provided detailed protocols for N- or C-terminal tagging in Drosophila cells...locus in C. elegans . This system uses a 10-day protocol, generates “clean” homozygous mutants with no ...

...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

...concentrating your virus. See our Concentrating AAV Protocol (Step 10) for instructions on how to do this. ...take below but you should read our AAV Production Protocol for additional best practices. Keep your cells...Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols for Work with Virus Watch Our AAV Purification...

...Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...2014) (Link opens in a new window) . protoSpaceJAM protoSpaceJAM is an all-around platform for CRISPR ...cloning; injection protocol pU6-BbsI-chiRNA ; phsp70-Cas9 PDF, 109 KB Orkin and Bauer Protocol for Genomic Deletions...Resources CRISPR Software General Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's... (Link opens in a new window) A collection of protocols, materials, and publications by members of the... library amplification CRISPR pooled libraries Protocol at Addgene Church gRNA design and cloning gRNA...pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and ssODNs...

...lentiviruses easily made in your own lab. We provide protocols and useful tips for generating your own lentivirus...using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...lentiviruses challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast...

... from it? The image in Figure 1 appears on our protocol page for AAV Purification by Iodixanol Gradient...post.  Here, we are presenting this image on a protocol page on our website, as an instructive step-by-step...chose this alt text: "The left panel is a profile photo of an iodixanol gradient after ultracentrifugation...

...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions...mammalian cells using 293T cell-based systems. Curr Protoc Immunol. 2001 May;Chapter 10:Unit 10.17C. doi: ...Research More resources on addgene.org Addgene Protocols: General Transfection The Addgene Retrovirus Guide...

...combine computational processing with biological protocols and revolutionary physical approaches. However...the structure in a similar way to the biological protocol, and assembly designs based around the use of ...commercially available tools and components. Rapid prototyping techniques such as 3D printing, on-demand CNC...

...selecting a bright fluorescent protein and a photostable fluorescent protein. In the third post of this...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...verify a number of key properties (brightness, photostability, oligomerization) of a number of promising ...

...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in...2018 and involved presentations and a handout on protocol sharing, material sharing, computational reproducibility...management plans, electronic lab notebooks, wet lab protocols, reagent sharing, bioinformatic tools, data and...

... area. Unless you are looking for a particular protocol or are trying to replicate an experiment, the ...to the methods section for finer points of the protocols. Are there any red flags about how the work was...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...

...Gamma-protocadherin-A3 [N144/5R] Gamma-protocadherin-A3 Mouse Mouse IgG2a 222155 Gamma-protocadherin-B2 ...Mouse IgG2a 164156 Anti-Gamma-protocadherin-C3 [N174B/27R] Gamma-protocadherin-C3 Mouse Mouse IgG2a 164157... 177503 Anti-Pan-Gamma-protocadherin-Constant [N159/5R] Pan-Gamma-protocadherin-Constant Mouse IgG2a 177504...IgG2a 182105 Anti-Pan-Gamma-protocadherin-A [N144/32R] Pan-Gamma-protocadherin-A Mouse IgG2a 182106 Anti-Histone...Mouse IgG2a 190299 Anti-Gamma-protocadherin-B2 [N148/30] Gamma-protocadherin-B2 Mouse Mouse IgG2a 190300...Mouse IgG2a 199398 Anti-Gamma-protocadherin-A3 [N144/17R] Gamma-protocadherin-A3 Rat Mouse IgG2a 199399 Anti-PINK1...Pan-Gamma-protocadherin-Constant recombinant mouse monoclonal antibody. Pan-Gamma-protocadherin-Constant...

... microbes? Try your hand at photography and participate in a science photo competition. Or nominate yourself...Indonesia. Since then, I have tried many things: photography, making videos, presenting in pubs and at science...

...CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...

... and delivers it cargo to certain cell types.  Botond Roska’s lab at the Institute of Molecular and Clinical...types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP...