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We narrowed to 331 results for: uros

Showing: 161 - 180 of 331 results
  1. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post
    ...placing FP timer DsRed-E5 under the control of Neurog3 (a gene controlling pancreatic differentiation)...preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric...
  2. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post
    ...cells where recombination has occurred. Neomycin, puromycin, and hygromycin resistance genes are commonly ...GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab.   Figure 3: A knockout...
  3. Academic vs. Industry Postdocs

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    Blog Post
    ...postdoc I’m currently doing an industry postdoc in neuroscience at AstraZeneca’s Waltham site after doing a ... and is applying this towards researching neurodegenerative diseases.   Additional resources on the Addgene...
  4. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post
    ...mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery...YFP, Strep, TEV-V5) with either blasticidin or puromycin selection. Researchers at the Allen Institute ...
  5. Cloning Mammalian Cells with the Agarose Method

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    Blog Post
    ...and endocrine cells such as AtT-20, PC12, Rin5F, Neuro2A and GH4C1, among others (see Lindberg papers in...of Medicine. Iris Lindberg is a professor of Neurobiology and Anatomy at the University of Maryland Medical...
  6. Simplify Cloning with in vivo Assembly

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    Blog Post
    ...Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular Biology....Laboratory of Molecular Biology. He is interested in neuronal signal transduction, and uses electron cryo-microscopy...
  7. The Challenges of Cell Culture

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    Blog Post
    ...Outreach Scientist at LGC Group, London, which is the European distributor for ATCC. He studied biochemistry ... high level of experience he now travels around Europe giving talks and seminars on cell culture and other...
  8. Plasmids 101: Control Plasmids

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    Blog Post
    ...pseudoreplication in neuroscientific studies: is it affecting your analysis? Lazic SE. BMC Neurosci. Jan 14;11:...
  9. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post
    ...calmodulin-dependent protein kinase II promoter Specific Used for neuronal/CNS expression. Modulated by calcium and calmodulin...expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA From the...
  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post
    .... https://doi.org/10.1038/ni.3608  Monnier P, Vigouroux R, Tassew N (2013) In Vivo Applications of Single...Antibody Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab Check out Addgene's...
  11. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post
    ...light-sensitive ion channels used to control activities like neuronal signaling — think of a mouse with an LED brain... — but there’s a lot more to optogenetics than neuronal activity. Researchers have designed a variety ...
  12. Five Popular Model Organisms

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    Blog Post
    ...important experimental model in the study of neurological diseases, such as Parkinson’s disease (PD). ...Sydney Brenner brought it into the lab to study neuronal development (Brenner, 1973). C. elegans are small...
  13. Evolution of Lab Techniques

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    Blog Post
    ...guest blogger, Krissy Lyon, a PhD candidate in Neuroscience at Harvard University. Just as computers, cell...Krissy Lyon! Krissy Lyon is a PhD candidate in Neuroscience at Harvard University currently studying the...
  14. Plasmids 101: Repressible Promoters

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    Blog Post
    ...the Q system from a gene cluster in the fungus Neurospora crassa. The QUAS promoter is less leaky than ...binary transcription systems in Drosophila.” Nat Neurosci 9(5) (2006): 703-9. PubMed PMID: 16582903. Potter...
  15. Antibodies 101: Introduction to Antibodies

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    Blog Post
    ...experiments in a variety of fields including neuroscience and immunology. They can be used for qualitative...Antibody Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab ...
  16. Brain Initiative Collection

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    Collection
    ...in a new window) NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection : The NeuroMab Facility and James...supports the development of biomolecular tools for neuroscience research. Browse the BRAIN Initiative collection...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to revolutionize...recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element...
  17. Allen Institute for Brain Science AAV Enhancer Collection

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    Collection
    ...-specific labeling. Neuron , 109 (9), 1449–1449. https://doi.org/10.1016/j.neuron.2021.03.011 PMID: 33789083...types and circuits. Neuron , 113 (10), 1507–1524. https://doi.org/10.1016/j.neuron.2025.04.035 PMID: 40403704...WPRE3-BGHpA AiP11390 DLX2.0 SYFP2 Pan GABAergic interneurons Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA...minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex 214518 pAAV-AiE2366m-minBG-SYFP2-WPRE3...WPRE3-BGHpA AiP1691 AiE2366m SYFP2 Lamp5 interneurons Isocortex 220737 pAAV-AiE2366m-minBG-iCre(R297T)...R297T)-BGHpA AiP20068 AiE2366m Cre Lamp5 interneurons Isocortex 224015 pAAV-AiE0724m-minBG-SYFP2-WPRE3-BGHpA...BGHpA AiP12863 AiE0724m SYFP2 Lamp5 interneurons Isocortex 224051 pAAV-AiE0724m-minBG-iCre(R297T)-BGHpA...
  18. Brain Armamentarium

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    Collection
    ...protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. Jonathan Ting...collection of Armamentarium tools will aid neuroscientists in studying circuits in laboratory animals ...technologies have the potential to transform neuroscience research by bringing the precision of molecular...dTomato_nlsdTomato AAV construct with dTomato driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_dTomato_nlsdTomato...Expression of CoChR-EGFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru 214862-PHPeB AiP14825...Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru 214858-PHPeB AiP14496...
  19. Plasmids for Stem Cell Research

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    Collection
    ...Fibroblasts Neurons Lentiviral Mouse Direct conversion of fibroblasts to functional neurons by defined ...Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons. Proc Natl...Fibroblasts Neurons Lentiviral Human MicroRNA-mediated conversion of human fibroblasts to neurons. Nature....Fibroblasts Cholinergic Neurons Lentiviral Human Small molecules enable neurogenin 2 to efficiently convert...cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons Lentiviral...molecules enable neurogenin 2 to efficiently convert human fibroblasts into cholinergic neurons. Nat Commun...Fibroblasts Medium Spiny Neurons Lentiviral Human Generation of Human Striatal Neurons by MicroRNA-Dependent...
Showing: 161 - 180 of 331 results