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We narrowed to 335 results for: uros

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Showing: 161 - 180 of 335 results

  1. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...cells where recombination has occurred. Neomycin, puromycin, and hygromycin resistance genes are commonly ...GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab.   Figure 3: A knockout...

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...selectively in Parvalbumin containing cortical interneurons. We now provide ready-to-use AAV vectors expressing...this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are now available as a...

  3. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery...YFP, Strep, TEV-V5) with either blasticidin or puromycin selection. Researchers at the Allen Institute ...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...and endocrine cells such as AtT-20, PC12, Rin5F, Neuro2A and GH4C1, among others (see Lindberg papers in...of Medicine. Iris Lindberg is a professor of Neurobiology and Anatomy at the University of Maryland Medical...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...placing FP timer DsRed-E5 under the control of Neurog3 (a gene controlling pancreatic differentiation)...preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric...

  6. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...postdoc I’m currently doing an industry postdoc in neuroscience at AstraZeneca’s Waltham site after doing a ... and is applying this towards researching neurodegenerative diseases.   Additional resources on the Addgene...

  7. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...A., Smith, C. J., Ottolini, M., Barker, B. S., Purohit, A. M., Grippo, R. M., Gaykema, R. P., Spano, A...magnetic control of the nervous system. Nature Neuroscience, 19(5), 756–761. https://doi.org/10.1038/nn.4265...

  8. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory of Molecular Biology....Laboratory of Molecular Biology. He is interested in neuronal signal transduction, and uses electron cryo-microscopy...

  9. The Challenges of Cell Culture

    Type
    Blog Post
    ...Outreach Scientist at LGC Group, London, which is the European distributor for ATCC. He studied biochemistry ... high level of experience he now travels around Europe giving talks and seminars on cell culture and other...

  10. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...pseudoreplication in neuroscientific studies: is it affecting your analysis? Lazic SE. BMC Neurosci. Jan 14;11:...

  11. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...calmodulin-dependent protein kinase II promoter Specific Used for neuronal/CNS expression. Modulated by calcium and calmodulin...expression than U6. May have better expression in neuronal cells. U6 small RNA expression shRNA From the...

  12. Evolution of Lab Techniques

    Type
    Blog Post
    ...guest blogger, Krissy Lyon, a PhD candidate in Neuroscience at Harvard University. Just as computers, cell...Krissy Lyon! Krissy Lyon is a PhD candidate in Neuroscience at Harvard University currently studying the...

  13. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    .... https://doi.org/10.1038/ni.3608  Monnier P, Vigouroux R, Tassew N (2013) In Vivo Applications of Single...Antibody Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab Check out Addgene's...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...light-sensitive ion channels used to control activities like neuronal signaling — think of a mouse with an LED brain... — but there’s a lot more to optogenetics than neuronal activity. Researchers have designed a variety ...

  15. Five Popular Model Organisms

    Type
    Blog Post
    ...important experimental model in the study of neurological diseases, such as Parkinson’s disease (PD). ...Sydney Brenner brought it into the lab to study neuronal development (Brenner, 1973). C. elegans are small...

  16. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...the Q system from a gene cluster in the fungus Neurospora crassa. The QUAS promoter is less leaky than ...binary transcription systems in Drosophila.” Nat Neurosci 9(5) (2006): 703-9. PubMed PMID: 16582903. Potter...

  17. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...experiments in a variety of fields including neuroscience and immunology. They can be used for qualitative...Antibody Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab ...

  19. Brain Initiative Collection

    Type
    Collection
    ...in a new window) NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection : The NeuroMab Facility and James...supports the development of biomolecular tools for neuroscience research. Browse the BRAIN Initiative collection...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative aims to revolutionize...recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element...

  20. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...-specific labeling. Neuron , 109 (9), 1449–1449. https://doi.org/10.1016/j.neuron.2021.03.011 PMID: 33789083...types and circuits. Neuron , 113 (10), 1507–1524. https://doi.org/10.1016/j.neuron.2025.04.035 PMID: 40403704...WPRE3-BGHpA AiP11390 DLX2.0 SYFP2 Pan GABAergic interneurons Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA...minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex 214518 pAAV-AiE2366m-minBG-SYFP2-WPRE3...WPRE3-BGHpA AiP1691 AiE2366m SYFP2 Lamp5 interneurons Isocortex 220737 pAAV-AiE2366m-minBG-iCre(R297T)...R297T)-BGHpA AiP20068 AiE2366m Cre Lamp5 interneurons Isocortex 224015 pAAV-AiE0724m-minBG-SYFP2-WPRE3-BGHpA...BGHpA AiP12863 AiE0724m SYFP2 Lamp5 interneurons Isocortex 224051 pAAV-AiE0724m-minBG-iCre(R297T)-BGHpA...
Showing: 161 - 180 of 335 results