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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,010 results for: RING;

Showing: 961 - 970 of 1010 results

  1. Science Guides

    Type
    Guide
    ...system in bacteria, have been modified for genome engineering. Find essential background information on CRISPR...Optogenetics Optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons...

  2. Addgene's eBook Collection

    Type
    Guide
    ...protein; information on FRET, biosensors, and monitoring protein localization; an introduction to non-...the many career options in science, advice on mentoring and management, resources for identifying your...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ...the library (electroporation and maxiprep). If delivering as virus, make virus; this creates a pooled lentiviral...Viral Vector resources . Check out our blog posts featuring some of our most popular libraries! Genome-wide...

  4. Promoters

    Type
    Guide
    ...Addgene's molecular biology reference material covering promoters, transcription and the transcription...the adaptor molecules that are responsible for bringing amino acids to the ribosome when proteins are ...

  5. Educational Resources

    Type
    Guide
    ...molecular biology techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid...

  6. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... Protocols Recovering Plasmid DNA from Bacterial Culture Recovering Plasmid DNA from Bacterial...with your plasmid Resuspension buffer Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated...solution. Add a renaturing solution to the denatured bacteria. Note: This step brings the pH back down...that is compatible with the next solution. Add a denaturing solution to the resuspended bacteria. Note: This...mM EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution II... II at room temperature Solution III - Renaturing Solution (Potassium Acetate) 120 mL 5M Potassium acetate...supernatant into a new tube by pipetting or carefully pouring. Optional: Add 5 μL of 2 mg/mL RNase A to the supernatant...

  7. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...a variety of applications. Sharing speeds science. We believe that sharing the full details of our protocols...mL luer-lock syringe, BD BD302832 0.2 µm luer-lock filter, VWR 431229 0.22 µm PES filtering system, 1000...1000 mL, VWR 431098 0.45 µm PES filtering system, 500 mL, VWR 430770 Trypan Blue, Thermo Fisher T10282 ... reagents Store at 4 °C until use. We suggest preparing fresh solutions after one month. BCD Feed 500 ... Glutagro Store at 4 °C until use. We suggest preparing fresh solutions after one month. 1000X protease...mL) Mix well and sterilize through a 0.2 µm PES syringe filter. Aliquot and freeze upright at -20 °C. Procedure...Section 3: BCD Feed and valproic acid supplementation During the 24–144 h post-transfection, supplement the ...

  8. Kit Free RNA Extraction

    Type
    Protocol
    ...to a refrigerated centrifuge, you can carefully bring a centrifuge into a cold room for centrifugation...centrifugation. Once you’re done using the centrifuge, bring this equipment back to room temperature, as prolonged...Add the correct amount of 7.5 M LiCl solution to bring the concentration of LiCl in the extracted aqueous...sample, consider making smaller aliquots of it and storing those in -80°C. Option #2 - TRIzol® Protocol Homogenize...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...siRNA selection tool to determine a set of top-scoring targets for your gene. For example, the Whitehead...alleviate concerns about off-target effects. B.2 Ordering Oligos Compatible with pLKO.1 To generate oligos... 5 μL 10x NEB buffer 1 1 μL AgeI add ddH 2 O to bring to 50 μL final volume Incubate at 37°C for 2 hours...ligase buffer 1 μL NEB T4 DNA ligase add ddH 2 O to bring to 20 μL final volume Incubate at 16°C for 4-20 ...for EcoRI 0.8 μL EcoRI 0.8 μL NcoI add ddH 2 O to bring to 20 μL final volume Incubate at 37°C for 1-2 hours...HEK-293T cells that were inadvertently collected during harvesting. TIP: In lieu of centrifugation, you...based on your experiment Detailed protocols for preparing polybrene, protamine sulfate, and puromycin are...

  10. Video Library

    Type
    Protocol
    ...step-by-step walkthrough of Addgene's ordering process Ordering Page MTA FAQs Answers to frequently asked...Plates Create plates to culture bacteria in the lab Pouring LB Agar Plates Protocol Streaking Bacteria on Plates...enzymes Diagnostic Restriction Digest Protocol Storing and Handling Addgene Plasmids What to do after ...receive your plasmids from Addgene Instructions for Storing and Handling Plasmids Genomic Deletions with CRISPR...Australia, and advice for current graduate students considering their future careers. Eric J. Perkins, PhD In...
Showing: 961 - 970 of 1010 results