...publications and funding, bioethics in the age of CRISPR, and the challenges of science becoming increasingly...rapidly changing world of genome engineering via CRISPR.  Though the individual topics are not surprising...scientific world? Would biohackers even be a thing if CRISPR hadn’t made manipulating the genome cheap and relatively...shocking. Mainstream media outlets have addressed CRISPR frequently over the last several years, but lay...politicization of science (from climate change deniers to anti-vaxxers), and the very real dangers of quackery,...scientists themselves.  The sections on optogenetics and CRISPR of course address some of the ethical implications...easy, fast, and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology...

... Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature...concentration of the antibiotic in culture, or remove the antibiotic entirely. If the antibiotic is reduced or...transfection. Some lentiviral vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which...stable cell culture after transduction. Performing antibiotic selection on transduced cells enables elimination...the transducibility of the cell line used, this antibiotic selection may be a vital step for obtaining a... Note that not all lentiviral vectors deliver antibiotic resistance. This protocol was established using..., treat target cells with a range of doses of antibiotic and determine the lowest dose that kills all ...

... the ZFNs.   Genetic Engineering Era: Post CRISPR  CRISPR The strategy of using a protein to recognize... Find more blog posts about CRISPR Learn more about the history of CRISPR Read about other genome editing...methods Resources on Addgene.org Visit our CRISPR guide Find CRISPR plasmids for your research Browse plasmids...during this period.  Genetic Engineering Era: Pre-CRISPR  Having visualized and understood the DNA, the ...breakthrough in the world of genetic engineering as CRISPR-Cas editing soon replaced the protein-based DNA-binding... (Langdon). The plot involves Langdon chasing an anti-villain geneticist named Zobrist who designs a virus... gene editors (meganucleases, ZFNs, TALENs, and CRISPR-Cas) is that they induce double-strand breaks (...

...Reactions Anti-drug antibodies (ADAs) are formed when the body recognizes a drug as a foreign antigen, triggering...s12929-019-0592-z  Resources Antibodies 101: Introduction to Antibodies CRISPR in the Clinic   ...replicate the complexity of antigen processing events that take place within an antigen-presenting cell like ...Beijnen, J. H., & Schellens, J. H. (2016). Antidrug Antibody Formation in Oncology: Clinical Relevance ...Monoclonal antibody drugs are popular therapeutics for a plethora of disease conditions, from cancer ...cancer to autoimmune disorders. Antibodies administered as drugs are still immunogenic, meaning that they elicit...of immunogenicity can help evaluate risk of an antibody.  In silico prediction of adverse reactions In...

... autophagy and, in a mouse model led to improved anti-cancer immunosurveillance and reduced tumor mass...development in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering...contrast to using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering... et al. "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection." Nature 543.7643...on novel cancer therapeutics such as  (Chimeric Antigen Receptors) CAR T cells and PD-1 modulators. An ... et al. "Caloric restriction mimetics enhance anticancer immunosurveillance." Cancer cell 30.1 (2016):...: eaal3118. PubMed PMID: 28495700. 4. Zelenay, Santiago, et al. "Cyclooxygenase-dependent tumor growth...

...the Addgene blog CRISPR Antimicrobials Treating Muscular Dystrophy with CRISPR CRISPR 101: Drug Discovery...initiated a CRISPR therapy for hATTR with the first-ever systemic CRISPR therapy. They engineered a CRISPR-Cas...Resources on Addgene.org CRISPR Plasmids and Resources CRISPR 101 eBook CRISPR History and Development ...post on CRISPR’s potential for correcting monogenetic diseases. Now, almost 10 years later, CRISPR’s potential...and editing strategies for CRISPR therapies. Systemic therapies involve CRISPR-Cas delivery directly to ...currently working on giving this treatment a CRISPR spin. CRISPR CAR-T treatments for leukemia, lymphoma, ...involving CRISPR Cas9 for these diseases was sponsored by Vertex Pharmaceuticals and CRISPR Therapeutics...

...BbvCI enzyme used for ssDNA preparation nicks the anti-sense strand, then the ssDNA template formed will...be sense. Therefore, mutagenic oligos need to be anti-sense so they will anneal to the template. The opposite... nicked for template formation: template will be anti-sense, primers should be sense. Does the orientation...Mutations in Your Plasmids Learn How You Can Use CRISPR Pooled Libraries to Generate Mutant Populations...Addgene.org Find Plasmids from the Whitehead Lab Use CRISPR Pooled Libraries to Generate Mutants ...

...multi-spacer CRISPR array, CRISPR-transposons can be multiplexed. Transcriptional repression (CRISPRi) in bacteria...Will Arnold. Although CRISPR systems were first discovered in bacteria, most CRISPR-based genome engineering...inefficiency? Use CRISPR to make it a selectable process! As NHEJ is ineffective in bacteria, CRISPR-induced double...eukaryotes in that CRISPR isn’t the primary editing force. In contrast, in E. coli, CRISPR is primarily a ...to directly modify a given gene. CRISPR-tranposons By combining CRISPR editing and transposons, the Sternberg...scenario allows for several potential CRISPR applications. First, CRISPR can be used to target locations in... Check out our CRISPR Featured Topic Page Learn more about Cpf1 Find out about CRISPR Multiplexing Resources...

...outperformed another CRISPR library with 6 sgRNAS, highlighting the benefit of sgRNA design in CRISPR libraries.... about CRISPR Kinome Libraries available at Addgene Learn about the advantages of pooled CRISPR libraries...genome-wide screening using CRISPR Resources on Addgene.org Find CRISPR plasmids and resources here ...genome-wide CRISPRko, CRISPRi, and CRISPRa libraries have been shown to outperform other popular CRISPR libraries...repressor (CRISPRi) or an activation domain (CRISPRa) to regulate gene expression. Some CRISPR libraries...generation libraries for genome-wide CRISPRa, CRISPRi, and CRISPR-Cas9 knockout genetic screens." bioRxiv...advantages of the optimized human CRISPR libraries. Brunello: human CRISPRko sgRNA library The Brunello library...

...sequences for antibodies are not shared. For instance, through open plasmid sharing, the CRISPR community ...Recombinant Antibodies? Antibodies are used by scientists around the world to detect, purify, quantify, deplete...of the antibody are encoded in a plasmid. (Right) An assembled antibody protein binds an antigen. Image...monoclonal antibodies as well as other forms of antibody-based (e.g., nanobodies, ScFVs) and non-antibody based...Recombinant Antibodies Recombinant antibodies offer numerous advantages over polyclonal antibodies and traditional...encoding antibodies, scientists will be able to make higher affinity antibodies, modify antibody function...I predict a similar revolution for antibodies. Recombinant antibody technology makes it easy for scientists...

... libraries Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR interference plasmids...plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...about other CRISPR methods for bacterial genome engineering   Learn about human CRISPRko, CRISPRi, and CRISPRa...them. For example, gene knockdown with CRISPR interference (CRISPRi) has been limited to lab-adapted bacteria...disease-associated. Mobile-CRISPRi: a modular approach for CRISPRi transfer Mobile-CRISPRi offers two advantages...knockdown experiments. Mobile-CRISPRi applications Mobile-CRISPRi expands CRISPRi to non-model bacteria including...introduce CRISPRi machinery into diverse bacteria species. Existing protocols can transfer CRISPRi into a...

..., allowing for quantitative analysis using different detection methods. Since the anti-GFP nanobody is...toolkit includes a set of plasmids each containing an anti-GFP nanobody fused with a different functional element...Sam Myers, a Scientist in the group, developed a CRISPR-based approach to detect proteins that associate... single domain antibody fragments typically engineered from heavy-chain only antibodies found in camelids...They possess many advantages over conventional antibodies: they are small (12-15 kDa), stable, highly soluble...still just as specific to their target as regular antibodies. These properties make nanobodies easier to transform...proteomics: developing and applying new technologies to quantify proteins in tissues, cells, and biofluids. Recently...

...light. Browse Plasmids: Anti-CRISPR Plan Your CRISPR Experiment Get Started CRISPR is a powerful system ...started using CRISPR in your own research. Generating a Knockout Using CRISPR You can use CRISPR to generate...lentiviral CRISPR libraries? Pooled lentiviral CRISPR libraries (often referred to as CRISPR libraries)...California, Berkeley. Anti-CRISPR In order to limit off-target effects of CRISPR-Cas9, researchers use... of small proteins called Acr (Anti-CRISPR) proteins to control CRISPR activity. Acr proteins are found...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (2nd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology...

...I was expecting when I set out to make my first CRISPR/Cas9 gene edit. I wanted to inactivate the gene...objective, it was surprisingly easy. It’s true, I CRISPRed. In this post, I’ll summarize the basic steps ...analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Cas9 delivery Overview: Before any editing...homologous recombination (Rago et al., 2007). Using CRISPR, I was able to knockout BRAF in a human cancer ... Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods... in Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Doench JG, Hartenian...maximize activity and minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, ...

...reversible CRISPR modification. In addition to these subpages, our updated CRISPR Guide and CRISPR History...Heaven: CRISPR and AAV Day 6: Bacteria: CRISPR Methods for Bacterial Genome Engineering and CRISPR Antimicrobials...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that’...popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR plasmid...tools: How to Design your gRNA for CRISPR Genome Editing and The CRISPR Software Matchmaker Day 2: Homology... repair: CRISPR 101: Homology Directed Repair and 3 Tips to Improve HDR Efficiency for CRISPR Editing ...Cpf1: A New Tool for CRISPR Genome Editing and Multiplex Genome Editing with CRISPR-Cpf1 Day 4: Edit validation...

...screening using CRISPR Find all of our CRISPR pooled library blog posts Check out all of our CRISPR blog posts... of CRISPR screen data that might be able to help you out. What is the Open Repository of CRISPR Screens... accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated...the Open Repository of CRISPR Screens BioGRID ORCS currently houses over 890 CRISPR screens in 629 cell ...   Include your CRISPR screen data in ORCS  Researchers who wish to have their CRISPR screen data included...Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR plasmids for your... post was contributed by the Open Repository of CRISPR Screens. Imagine you’ve just discovered that your...

...about CRISPR Pooled Library Experiments Additional resources on the Addgene website Find CRISPR Plasmids...on your CRISPR background with our CRISPR Guide Find Software Tools for gRNA Design    ...updated in 2022 by Susanna Stroik.  The advent of CRISPR/Cas9 has made it easier than ever to make precise... be found in “The PAM Requirement and Expanding CRISPR Beyond SpCas9.” Single base pair edits Is the location...design programs. Find Validated gRNAs for Your Next CRISPR Experiment Cas9 expression systems Addgene carries...the various delivery systems can be found here “CRISPR 101: Mammalian Expression Systems and Delivery ...genome edit can be found in our blog post entitled “CRISPR 101: Validating Your Genome Edit”. Is Cas9 not ...

...Endothelial-specific AAV delivery Recombinant anti-FLAG tag antibodies Peptide-assisted genome editing for ...38582-7. PMID: 37291094.   Recombinant anti-FLAG tag antibodies By Ashley Waldron Of all the epitope tags...original mouse Fab region with rabbit Fc) anti-FLAG antibody, both of which are also available in trial... a variety of applications. Find anti-DYKDDDDK recombinant antibodies here!...cells By Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells ...months we highlight some of the new plasmids, antibodies, and viral preps in the repository through our...Importantly, AAV1-X1 is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), ...

...retroviral mouse CRISPR knockout library Article contributed by Alycia Bittner CRISPR pooled libraries...method used to introduce the gRNAs into cells. Most CRISPR pooled libraries have been created in lentiviral...by lentivirus, and thus screening with available CRISPR libraries was not possible. Primary murine T cells...Sarah Teichmann and colleagues created a retroviral CRISPR knockout library using gRNAs designed by the Kosuke...factors as the screen readout. This retroviral mouse CRISPR pooled library can be used for screening of cells...in cells with few genetic tools. Find the mouse CRISPR knockout library at Addgene. Henriksson et al.,...31366908 Adapting sigma54-dependent transcription for CRISPR activation in bacteria Article contributed by Will...

...became much more realistic with the advent of CRISPR. Using CRISPR/Cas9 SAM to "shock and kill" To “shock ...HIV-1 out of the genome with CRISPR/Cas9 Before the introduction of CRISPR, HIV-1 genome editing with ...Treating Muscular Dystrophy with CRISPR Gene Editing Learn to Use CRISPR Pooled Libraries for Genome-Wide...updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ...second challenge for CRISPR HIV-1 therapies is sequence specificity. Whereas antiviral therapies target HIV... to vanquish these hidden viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel...University has demonstrated two potential strategies for CRISPR-HIV therapeutics - one using dCas9-SAM to activate...