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We narrowed to 8 results for: Bioid

Showing: 1 - 8 of 8 results

  1. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...The Split-BioID method Enter Split-BioID, a spatiotemporally controllable version of the BioID protocol... using Split-BioID-GFP and BioID run on six unrelated proteins. One caveat of Split-BioID is that the ...Advantages of Split-BioID Split-BioID’s key advantage over other PPI methods, including BioID, is its spatiotemporal...considerations for Split-BioID It’s important to remember that with Split-BioID, and BioID, any protein that’...proximity-dependent biotinylation technique BioID. The key advantage of Split-BioID is that it allows for the validation...protocol. The critical component for both BioID and Split-BioID is BirA*, a promiscuous version of BirA, an ...Figure 1: Using Split-BioID to Identify Protein Protein Interactions. Split-BioID is a protein complementation...

  2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ... technique, BioID, was developed in 2012 in the Brian Burke Lab (Roux et al., 2012). BioID utilizes the...Addgene! Since the development of BioID and APEX, optimized versions of BioID and APEX have been developed ...Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-BioID combines...Addgene.  BioID2: A smaller improved promiscuous biotin ligase The Roux Lab in 2016 optimized BioID to create... for promiscuous biotinylation. Compared to BioID, BioID2 exhibits enhanced proximal labelling, requires...promiscuous biotin ligases (Branon et al., 2018). BioID and BioID2 can take hours to label proteins and APEX2...affinity purification/mass spectrometry methods.  BioID: a promiscuous biotin ligase that can efficiently...

  3. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...enzymes most frequently used for PL were APEX2 and BioID. APEX2 is fast, tagging proximal proteins within...requires H2O2, which can be toxic in living cells. BioID was derived from biotin ligase and uses far less...evolution technique to the biotin ligase from which BioID was derived, creating a pair of enzymes that overcome...the speed and toxicity limitations of APEX2 and BioID. In the Ting Lab’s recent Nature Biotechnology paper...function across a much broader temperature range than BioID, which only functions optimally at 37℃. The increased...

  4. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ... Split-BioID Find resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids...-protein interactions (yeast-two hybrid, Co-IP, BioID),  and that’s just to name a few. But what if you...RaPID borrows the E. coli biotin ligase BirA* from BioID and allows a researcher to identify proteins that...interactions with RaPID RaPID is similar to the BioID platform for identifying protein-protein interactions...was  compared head-to-head with the E. coli and BioID2 BirA*s in the context of RaPID for their ability...when compared to a scrambled RNA motif control). BioID2 had no detectable signal at the same time point...

  6. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...fusion proteins Learn how biotin is used in Split-BioID: An improved method for studying protein-protein...

  7. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...-dependent biotin identification (BioID) MCS-BioID2-HA or myc-BioID2-MCS - To fuse your protein of interest...interest to the N-terminus or C-terminus of BioID2 3xHA-TurboID-NLS_pCDNA3 or 3xHA-miniTurbo-NLS_pCDNA3...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...The Roux Lab’s new and improved version of BioID, called BioID2, is significantly smaller, allows for more... has deposited HA tagged BioID2 for N-terminal fusions and Myc tagged BioID2 for C-terminal fusions. ...enhanced labeling of proximate proteins. Overall BioID2 improves the efficiency of screening for protein–protein...
Showing: 1 - 8 of 8 results