Split-BioID: An Improved Method for Studying Protein-Protein Interactions
...Split-BioID is BirA*, a promiscuous version of BirA, an E. coli biotin ligase. In BioID, BirA* is fused to...-BirA*) and C-terminal BiraA* (C-BirA*). These two fragments are fused to two proteins, here proteins ...occurs, BirA* activity is restored, and nearby proteins are labeled with biotin. For labeling, BirA* fusion...where BirA*, an E. coli biotin ligase is split into two non-functional fragments: N-terminal BirA* (N-BirA... interact, the two BirA* fragments complement each other to form a functional BirA* enzyme. When this ...within a ~10 nm radius of the bait. In Split-BioID, BirA* is first split into two non-functional fragments... other, they combine forces to form a functional BirA* enzyme. This type of experiment is called a protein...