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We narrowed to 94 results for: Blue fluorescent Protein

Showing: 1 - 20 of 94 results

  1. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post
    ... Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All... All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...understand how protein localization changes over time.Hallmarks of fluorescent protein timers When FP... as part of protein fusions or expressed independently) originally fluoresce green or blue and, when they...expressing a single FP-timer protein or protein fusion. This one protein method simplifies cloning and...display progressively longer blue fluorescence maturation times and varied blue-red maturation times (Table...HisB-Fast-FT Medium-FT 401 (blue), 579 (red) 464 (blue), 600 (red) 18.4 (blue), 5.8 (red) 2.7/4.7 3.9...

  2. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post
    ...Photoactivatable fluorescent proteins (PA-FPs) are fluorescent proteins that display unique changes in their spectral...Blog Read More about Fluorescent Protein Imaging Choosing Your Fluorescent Protein for Multi-Color Imaging...Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate...from low fluorescent states to high fluorescence states, they can change from one fluorescent color to...photoconvertible fluorescent proteins (PC-FPs). PC-FPs can convert from one fluorescent color to another...Selected Irreversible Photoactivatable Fluorescent Proteins Protein Oligomeric State Activating Light ...Selected Reversible Photoactivatable Fluorescent Proteins Protein Oligomeric State Activating Light ...

  3. Fluorescent Proteins 101: Fluorescent Biosensors

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    Blog Post
    ... of fluorescently-labeled components Fluorescence-activated cell sorting (for cells); fluorescent HPLC...and fluorescent reporters being especially popular. We’ll give you an introduction to fluorescent biosensors...and zinc. Some biosensors contain a single fluorescent protein fused to a domain that senses the concentration...structural organization and activating the fluorescent signal. Blue flame GECO biosensors from Robert Campbell...biosensors. Blue flame “cameleon” calcium sensors created by Roger Tsien’s lab contain two fluorescent proteins...that increases FRET transfer between the fluorescent proteins. FRET is highly amenable to biosensor creation...Examples include blue flame mitochondria-targeted calcium sensor CMV-mito-GEM-GECO1  and blue flame extracellular...

  4. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post
    ... like chromoproteins, fluorescent proteins, and luminescent proteins. While fluorescent proteins and luminescence...used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to blue to ultraviolet...whereas chromoproteins do not fluoresce at all or fluoresce at very low levels. Chromoproteins in nature...inquiries.  What is a chromoprotein and how is it different from fluorescent proteins? Before going into ...into the difference between chromoproteins and fluorescent proteins, it’s important to note that despite ...distinction by name, chromoproteins are actually a subset of the fluorescent protein family. What sets them...to the history of fluorescent proteins in molecular biology research, chromoproteins have a shorter history...

  5. Fluorescent Proteins 101: History of Fluorescent Proteins

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    Blog Post
    ...reach. Nobel Prize for fluorescent proteins The importance of fluorescent proteins was recognized in 2008...learning more about fluorescent proteins, I invite you to download our Fluorescent Protein eBook and learn..., as well as fluorescent ones, like Green Fluorescent Protein (GFP). These molecules occur naturally in...the light they emit. Proteins that give off light include chemiluminescent proteins, like luciferases, .... The discovery of GFP The story of how fluorescent proteins like GFP came to be used as research tools...green glowing protein could be used to measure gene transcription, as well as to track protein localization...function and shift its emission spectrum. New fluorescent proteins with new colors Around the same time, researcher...

  6. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ...Special Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones...Structure Plasmids Fast-FT 403(blue), 583(red) 466(blue), 606(red) 14.9(blue), 6.8(red) 2.8/4.1 7.1 hr (red...Bacterial Expression Medium-FT 401(blue), 579(red) 464(blue), 600(red) 18.4(blue), 5.8(red) 2.7/4.7 3.9 hr (red...Bacterial Expression Slow-FT 402(blue), 583(red) 465(blue), 604(red) 11.7(blue), 4.2(red) 2.6/4.6 28 hr (red... backbones with different fluorescent tags for you to create fusion proteins with your gene of interest...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection... Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer...

  7. Michael Davidson Fluorescent Protein Collection

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    Collection
    ...Michael Davidson Fluorescent Protein Collection Michael Davidson Fluorescent Protein Collection More Fluorescent...lab fluorescent protein fusion constructs and backbones. Plasmid... Plasmid Collections Fluorescent Proteins Michael...collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian...empty backbone with different fluorescent tags for you to create fusion proteins with your gene of interest...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The lab of Michael...more information. Empty Backbones mEmeralds Reds Blues Cyans Oranges Greens Yellows Photoactivatables Empty...

  8. Hot Plasmids - October 2020

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    Blog Post
    ...inhibitory synaptic proteins Fluorescent protein system for C. elegans Fluorescent timers for studying..., a fluorescent proteins system for Caenorhabditis elegans By Jennifer Tsang Fluorescent proteins (FPs...neurotransmitters. Using genetically encoded fluorescent proteins coupled with Fibronectin intrabodies generated...synaptic proteins allowing them to localize to the synapse and minimize extraneous fluorescence from other...By combining vectors expressing different fluorescent proteins targeting inhibitory or excitatory synapses...are not fluorescent on their own. When the two fragments come together, they fuse and fluoresce. The team...the target protein. The team validated this tool by targeting six different genes whose protein products...

  9. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post
    ...the core of the protein. OptoNBs were also tagged with an infrared fluorescent protein tag which let the...between dark and blue light which revealed a blue light-dependent shift in mCherry fluorescent to the surface...choose your own protein purification adventure. You want to purify your favorite protein (YFP). You have...with blue LED lights. When you switch off the lights, OptoNBs bind YFP and non-specific proteins flow ...nanobodies whose binding of their target protein is altered by blue light illumination. This ability to reversibly...bind and release a protein of interest in response to light allows for tagless protein purification as well...dark-induced dark-induced Loop 6 blue-light induced dark-induced blue-light induced Table 1: Summary...

  10. Which Fluorescent Protein Should I Use?

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    Blog Post
    ...categories of fluorescent proteins Photoactivatable / Photoconvertible: These proteins can switch their...state of the protein is non-fluorescent, thus allowing very low background level of fluorescence. Examples... photoconvertible proteins are PA-GFP, Dendra2, and the mEOS proteins. Some proteins are reversibly switchable...you, "Which fluorescent protein should I use?" And if you're looking for more fluorescence microscopy ...Interactive Visualization of Fluorescent Protein Properties  Fluorescence Spectrum Viewer  from BD bioscience...processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov et. al. (2010) “Fluorescent proteins and their applications...Haimovich of greenfluorescentblog. Be honest. Do you really know how fluorescent proteins glow?   Fluorescent...

  11. CRISPRainbow and Genome Visualization

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    Blog Post
    ...about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins Control...Control Protein Activity with GFP Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent...Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ...vivo. Some researchers have used fluorescently tagged DNA-binding proteins to label certain loci, but this...image represents the composite of fluorescent measurements from red, blue, and green channels overlaid on.... Each ortholog was fused to a different fluorescent protein to create three distinct colors. The specificity...containing two hairpin sequences, which recruit fluorescent proteins BFP, GFP, and RFP fused to the respective...

  12. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    Blog Post
    ...TagRFP-T fluorescence intensity due to interactions with photoactivatable fluorescent protein Dronpa. ... dark yellow fluorescent protein (YFP) with an excitation peak of 519 nm. These proteins have improved... efficient oligomerization of target proteins in response to blue light. This tool, CRY2clust, was created...basic-helix-loop-helix) protein. After fusing the CRY2 and CIB1 domains to separate proteins of interest, a researcher...with blue light.  Similarly, light can also be used to control homo-oligomerization of a protein fused... oligomerization when fused to a protein of interest. Fusion protein oligomerization happens within seconds... in TagRFP-T fluorescence intensity at different time points, the authors tracked protein activity while...

  13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ...Bioluminescence and fluorescence from proteins such as Green Fluorescent Protein (GFP) has likely existed...applications in the lab. Why Green Fluorescent Protein? GFP is a ~27 kDa protein consisting of 238 amino acids...study protein-protein interactions. In this case, two portions of EGFP are fused to the proteins of interest... in fluorescent microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide...acting as a biosensor or cell marker, studying protein-protein interactions, visualizing promoter activity... any organism while still maintaining fluorescence. The protein structure, first reported in 1996, is ...of the entire GFP protein, which is almost completely required to maintain fluorescent activity; very little...

  14. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...the photoswitchable proteins offers a powerful approach to control protein-protein interactions in cells...light-sensitive protein domains, they have developed a system to control CRISPR-Cas9 activity using blue light ... Acr protein AcrIIA4. In the dark, AcrIIA4 inhibits CRISPR activity.   With the addition of blue light...responds to blue light quickly. Within minutes of inactivating the CASANOVA complex, fluorescent CRISPR complexes... Biol 2018. PubMed PMID:29483642. Making protein-protein interaction studies easier with a 2in1 vector...The 2in1 cloning system is well suited for protein-protein interaction (PPI) studies in plants that rely...25971551. Discovery of new protein binder tools for photoswitchable proteins cPYP and AsLOV2 Article contributed...

  15. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    Blog Post
    ...about these proteins is that they are monomeric fluorescent proteins. Fluorescent proteins that oligomerize...plasmids containing the fluorescent protein mNeonGreen. This fluorescent protein joins mTFP1 and mWasabi...: a yellow-green fluorescent protein mNeonGreen is a yellow-green fluorescent protein that was derived... Read our fluorescent protein blog posts Find blog posts about choosing fluorescent proteins for your ...Michael Davidson fluorescent protein collection Check out Addgene’s fluorescent protein plasmids and resources...yellow fluorescent protein wavelengths, it can be imaged either with standard green fluorescent protein... other fluorescent proteins. Find mNeonGreen plasmids here! mTFP1: a monomeric teal fluorescent protein...

  16. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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    Blog Post
    ...modified EB1 protein are overexpressed in cells, microtubule growth can be switched off with blue light which...fluorophore binding aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging...always on the lookout for better, brighter fluorescent proteins (FP) with improved tissue penetrance and...controls the activity of a microtubule-associated protein to regulate cell growth dynamics Article contributed...with high affinity in the dark but dissociate under blue light. This tool has now been modified by Torsten...normally sits on microtubules to attract other proteins involved in their growth and extension. Now, they...conformational changes, and as a consequence, EB1 protein function is inactivated by the separation of its...

  17. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post
    ... Post on Protein Tags Read Other Posts on Fluorescent Proteins Browse Our Fluorescent Protein Pages Find...the untagged protein of interest. To avoid the accumulation of dark fluorescent proteins in your experiments...integral membrane proteins and membrane associated proteins (i.e. GPI-anchored proteins), which are more...Cysteineless non-glycosylated monomeric blue fluorescent protein, secBFP2, for studies in the eukaryotic...applicable to many microscopy experiments with fluorescent proteins (FPs) localized to compartments other than... cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing a bright signal for the... of the fusion protein molecules fail to correctly fold? Misfolded FPs do not fluoresce. A dark population...

  18. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...post: Blue-light inducible Cre for use in bacteria CRISPR-Casɸ mGold, a new yellow fluorescent protein...and joining the protein regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes...32675376/ mGold, the most photostable yellow fluorescent protein to date By Justin Ng High-throughput screening...cell types expressing variants of Yellow Fluorescent Protein (YFP). The team identified a new YFP variant...plasmids New items from the viral service A fast blue-light inducible Cre for precise control of bacterial...use light to induce conformational changes in a protein.    Cells expressing an RFP reporter plasmid...plasmid for recombinase activity were exposed to blue light. Cre-Vvd showed substantially improved activity...

  19. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post
    ... of fluorescent proteins requires some thought. The first step in choosing fluorescent proteins for ... be aware of what fluorescent proteins are available. With new fluorescent proteins being published every... Choosing compatible fluorescent proteins To choose a set of fluorescent proteins to be imaged together...crosstalk between the two fluorescent proteins. If you must image fluorescent proteins whose spectra overlap...infrared fluorescent protein such as iFP1.4 or iFP2.0. Beware that these infrared fluorescent proteins require...with fluorescent proteins. With the right microscope and the right choice of fluorescent proteins, imaging...follow multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires...

  20. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    Blog Post
    ...chimeric protein consisting of Cas9 and a photo-dissociable dimeric green fluorescent protein (pdDronpa...CRY2) and calcium and integrin-binding protein 1 (CIB1) proteins. The goal of both groups was to create...composed of two fusion proteins: 1) the genomic anchor - an inactive, dead Cas9 protein (dCas9) fused to CIB1...figure below. Once triggered by blue light, the CRY2 and CIB1 proteins heterodimerize and move the activator...form a functional protein due to being fused with high-affinity interacting proteins Coh2 and DocS, respectively...particular interest since blue light has limited applications in vivo. UV and blue light is readily absorbed... saCas9 protein. Stop light! Photoactive anti-CRISPRs halt Cas9 activity Anti-CRISPR proteins are a highly...
Showing: 1 - 20 of 94 results