We narrowed to 45 results for: COB

...coli , Mycobacterium smegmatis Carolyn Bertozzi , Jessica Seeliger 202406 pJYP1 Riboswitch Cobalamin (vitamin...Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis Sabine Ehrt 44561 pST-KT Pmyc1/...Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis Vinay Nandicoori 78577 pL99 PnitA-NitR...Acetamidase promoter Acetamide Escherichia coli , Mycobacterium smegmatis Matthias Wilmanns 84692 pMyC-kan Acetamidase...Acetamidase promoter Acetamide Escherichia coli , Mycobacterium smegmatis Matthias Wilmanns 84689 pMyBADC-kan...pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis Matthias Wilmanns 17806 pPro18 pPrpB Propionate...pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis Matthias Wilmanns 44447 pLC290 pR/...

...for transcriptional silencing Mycobacterium tuberculosis and Mycobacterium smegmatis. In addition to their... “pCRISPomyces” systems for use in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA...efficiencies or can be proteotoxic when used in Mycobacterial species, Sarah Fortune’s lab screened 11 other... 32:1141–1145 . https://doi.org/10.1038/nbt.3011 Cobb RE, Wang Y, Zhao H (2014) High-Efficiency Multiplex...2016) Investigating essential gene function inMycobacterium tuberculosisusing an efficient CRISPR interference...

... These tools consist of cobalamin-binding riboswitches that bind to cobalamin-fused fluorescent dyes; ...; binding of the cobalamin fusion to the riboswitch results in increased fluorescent signal. Prior to ...fluorophores. You can find plasmids containing the cobalamin binding riboswitches used in the Riboglow platform...

...researchers. Modeling CRISPR We were excited when Jacob Corn contacted us in 2014 to ask if we could make... project can be a dangerous thing. Fortunately, Jacob was able to quickly explain that it was common knowledge...conformation that was not “poised for cleavage”. Jacob computationally added the non-template strand to...an effective teaching tool. You can read about Jacob’s initial reaction to this model on his blog. Or,...

...Gray) and two MECP2 labs (Gail Mandel and Stuart Cobb) to create the Gene Therapy Consortium. Leveraging...10.1016/j.cell.2013.01.038 Guy J, Gan J, Selfridge J, Cobb S, Bird A (2007) Reversal of Neurological Defects...Gadalla KKE, Heindel C, Chen D, Zaric V, Bailey MES, Cobb SR, Gray SJ (2017) Improved MECP2 Gene Therapy Extends...Koerner MV, Gadalla KKE, Guy J, De Sousa D, Hector RD, Cobb SR, Bird A (2017) Radically truncated MeCP2 rescues...

...10.1093/genetics/iyae126.   Gene disruption in Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system...dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium abscessus, an increasingly common human pathogen...-based systems have been developed for other mycobacteria, but such systems don’t always work across species...for rapid and efficient genetic disruption in Mycobacterium abscessus. J Bacteriol., 206(3), e0033523. https...

...Understanding drug resistance mechanisms of Mycobacterium tuberculosis Dr. Patricia Sheen, Universidad...interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance to pyrazinamide (PZA...methods and improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies ...

...The following post was contributed by Derek Jacoby from Makerspace Victoria, CA Over the decades science...among our members we’ve managed to learn a lot and cobble together quite a diverse array of capabilities....contact your local space and get involved! Derek Jacoby is a PhD student at the University of Victoria....

...This post was contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding...versatile technique. Many thanks to our guest blogger Jacob Lazarus from Harvard. Jake received his MD and a...2014) :148–153. [accessed 2019 Feb 28]. Lazarus, Jacob E., et al. "A New Suite of Allelic Exchange Vectors...

... Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 (Cas9) is an RNA-guided ...Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.   ...

...Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222 pPBT-PE2-PuroTK-pegRNA_GG... pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm Mikkelsen 174038 pU6-tevopreq1-GG-acceptor...

...Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium sp. Neisseria sp. Pseudomonas...

...during the development and disintegration of mycobacterial granulomas." Immunity 28.2 (2008): 271-284. ...presentation and T cell effector function in mycobacterial granulomas." Immunity 34.5 (2011): 807-819. ...

...scientific endeavor October 2-3, 2014 Boston University Jacob Sleeper Auditorium, Boston, MA Register on Eventbrite...

...Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper, etc.), allowing His fusion proteins to...

... Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab, describes how you can better design ...

... Genome Editing Chris Richardson and members of Jacob Corn’s lab describe how a better understanding of...

...10.1177/002215549804600813  Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical technique ...

...chromosome” was proposed a few years later by Élie Jacob and François Wollman and became the widely adopted...

...26808669. PubMed Central PMCID: PMC4916159. 2. Jacobson, K., Rajfur, Z., Vitriol, E., & Hahn, K. (2008...