... cloning of firefly luciferase was published in 1985, several studies utilized luciferase as a genetic... Biological sources of luciferase Although luciferase isolated from the firefly beetle (Photinus pyralis... plasmids expressing luciferase Luciferase reporter assay The typical luciferase-containing plasmid is...second most commonly used luciferase. Other organisms from which luciferases have been cloned from include... North American firefly(Photinus pyralis) 560 61 Luciferin ATP No Japanese firefly(Luciola cruciate...cruciate/lateralis)Italian firefly(Luciola italica)Southern Russian firefly(Luciola mingrelica) 562 64 Luciferin...containing luciferase and the gene of interest permits translation of functional luciferase, until miRNA...

...pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF) Firefly/Renilla Creating ORF-Firefly luciferase...pGreen2x35SLUC Firefly/Renilla Creating ORF-Firefly luciferase gene fusions in plants. Renilla luciferase under... Firefly CMV Mammalian expression of firefly luciferase William Kaelin 21375 pHIV-Luciferase Firefly EF1α...118017 pLX313-Firefly luciferase Firefly EF1α Lentiviral expression of firefly luciferase William Hahn,...types of luciferase found in Addgene’s collection are Firefly luciferase (Fluc), Renilla luciferase (Rluc...Taipale 87077 pLenti6.2-Firefly-ccdB Firefly Creation of N-terminal Firefly luciferase fusions using Gateway... pGWB-nLUC Firefly Creation of N-terminal Firefly luciferase fragment for split-luciferase complementation...

...fusion proteins from any ORF available as a standard entry clone. ORFs from an ORFeome Gateway library or any...Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in ...alternative to the widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids... issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash...insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation of its substrate, luciferin...Santalucía, you can now take any open reading frame (ORF) and with a simple recombination reaction, generate... any PCR-amplified ORF cloned into pDONR221 can be expressed either without a tag or as multiple fusions...

...TALEN array for genomic engineering Luciferase Reporter See our Luciferase collection of plasmid backbones... to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein... - Tet-inducible HA-tagged lentiviral vector for ORF expression pDest-565 - Gateway destination vector...vector for gene expression pINDUCER21 (ORF-EG) - Inducible lentiviral gene expression vector...