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We narrowed to 11 results for: Flp frt flex

Showing: 1 - 11 of 11 results

  1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient The first... or one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors – inversion...fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression...Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels...levels of genetic control. What is FLP FRT and how does it work? FLP recombinase, which is named after ... The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes FRT sites...mediated by FLP or Cre are reversible. Whereas the excision of a piece of DNA flanked by loxP/FRT sites is...

  2. Plasmids 101: FLEx Vectors

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    Blog Post
    ... on the expression of another! What Are FLEx switches? FLEx (or “flip-excision”) switches were designed...events: Cre recombinase binds loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by target... powerful FLEx switch can be created for your specifc experimental needs. How does the FLEx switch work...genetic FLEx switch that turns BFP expression off, while turning on mCherry expression. For this FLEx switch...pair of loxP sites and a pair of FRT sites and include both Cre and FLP recombinases.   Figure 1...Beyond switching fluorophores, why is FLEx useful? The power of the FLEx system lies in the ability to conditionally...Today’s post will focus on one such strategy--the FLEx switch--which utilizes recombination elements to...

  3. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...specific sequences, lox (recognized by Cre) or frt (recognized by Flp), to either flip or excise a gene that ... vector designs used a lox-STOP-lox (LSL) or frt-STOP-frt (FSF) sequence prior to an ATG start codon to...with AAV DIO/Flex vectors and leaky expression can occur in cells that don’t express Cre or Flp, what can...transgenic models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent...optimize experiments to minimize it. Overview of DIO/FLEx vector design Recombinase-dependent vectors utilize...  As such, researchers developed new tools called FLEx/DIO vectors where the transgene is floxed by two...inverted open-reading frame (DIO), or FLip-EXcision (FLEx) vector. The two recombination sites are both recognized...

  5. Cre-lox system

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    Collection
    ...orientation of the FRT sites. FlpO constructs Addgene Blog Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist...Shivdasani 18925 pJ241-FLEX FLEX switch empty backbone Sternson 38042 pAAV-CA-FLEX Cre activates gene of...multiple tissues. FLP/FRT: Alternative recombinase system for the control of gene expression. Flp recombinase...Cre rTH AAV Wilson 108454 mCherry-p2A-CreERT2-FRT-kan-FRT For in vitro transcription of Cre or to recombine... interest makes FLEx switch a powerful experimental tool. For more information on FLEx switch and what...recombinase recognizes FRT sites. Flp binding to these sites can result in excision, inversion or translocation...Plasmids Addgene's Blog: Cre-lox Addgene's Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs ...

  6. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...? This is where FLEx enters the scene. FLEx: A light when night is about you! The FLEx (for flip-excision...as Cre or Flp that induce DNA recombination at defined recognition sites (i.e., loxP and FRT, respectively... neuroscience, an era in which technologies like FLEx and optogenetics meet, allowing scientists to investigate...Christenson Wick and Krook-Magnuson, 2018). Optogenetic FLEx vectors contain a strong promoter upstream of an...transcribed (Figure 2) (Sharma and Zhu, 2014). How does FLEx enable strong opsin expression in specific neuronal...using Cre-dependent viruses, such as the optogenetic FLEx vectors in combination with transgenic animals or...strong promoter only in specific neuronal cells. FLEx ensures, therefore, spatial accuracy and strong ...

  7. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Control Gene Expression with FLEx Vectors Resources...

  8. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also available in AAV1. Plasmid...-mCherry Karl Deisseroth 83895 AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Gordon Fishell   Optogenetics Optogenetics...expression. Plasmid 75470 is now available in AAV1 for Flp-dependent expression of ChR2(H134R) bound to an mCherry...pAAV-EF1a-C-CreintG Connie Cepko 75469 AAV1 pAAV-EF1a-Flp-DOG-NW Connie Cepko Viral vectors coming soon...targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral preps SARS-CoV-2 protein... Serotype Name Depositor 75470 AAV1 pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry Connie Cepko 83898 AAVrg ...

  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... GAL4 architecture or combined the system with Flp/FRT, as reviewed by del Valle Rodriguez et al.   LexA...working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline ...

  10. Optogenetics AAV Preps

    Type
    Collection
    ...fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive Serotype AAV1 AAV2...Deisseroth 22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato CAG...pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG Jaws GFP Cre dependent 1, 5, rg* Boyden 84446 pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2... Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry CAG ChR2/H134R mCherry Flp dependent 1 Cepko 55639... Boyden 130909 AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] CAG ChrimsonR tdTomato Flp dependent 8 Boyden 100049...pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2 mCherry Cre and Flp dependent 8 Deisseroth 137143 pAAV-nEF-Con/Foff 2.0... pAAV-nEF-Coff/Fon-ChR2-mCherry nEF ChR2 mCherry Flp dependent 8 Deisseroth 137163 pAAV-nEF-Con/Foff 2.0...

  11. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... three new versions. Flipbow employs the Flp recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing...for those involving recombination elements such as FRT or Lox sites, and provides a modular system for easier...fusion or recombination vectors. The efficient and flexible cloning process provides improvements to classical..., thus forming a functional genetic circuit. Flexibility has been built into the Level 2 vectors to allow...
Showing: 1 - 11 of 11 results