Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
Type
Blog Post
...lactose released activates expression of GFP from the lac operon. GFP expression can then be used to determine... make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to oligomerize when...To do so, she generated fusion proteins in which Dronpa domains flanked either side of an enzyme’s active...ultraviolet light exposure caused oligomerization of the Dronpa domains thereby occluding the enzyme active site...the enzyme. Later exposure to cyan light stopped Dronpa oligomerization and turned the enzymes back on....optimized these first generation FLiPs by mutating Dronpa to lower its aggregation tendency, increase its...iterations of FLiPs only used N- and C-terminal dronpa fusions.
With these various designs, Xin has been...