...inferes with DNA replication by binding the GyrA subunit of DNA gyrase and trapping it in the cleaved complex...

... Cells cannot grow on galactose only gyrA, gyrA96 DNA gyrase mutation Confers resistance to nalidixic...the ccdB gene (important in Gateway cloning). F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB-, mB...white screening. F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17...cloning repetitive DNA. endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F' traD36 proAB+...potential to recombine.  F- endA1 glnV44 thi-1 recA1 gyrA96 relA1 Δ(lac-proAB) mcrA Δ(mcrBC-hsdRMS-mrr) λ-  ...amplification. F' mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 gal-thi-1 supE44 λ-relA1 Δ(lac-proAB)/F' proAB+...routine cloning, nalidixic acid resistant. endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F'[ ::Tn10 proAB...

...protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded DNA ....1, which contains a mutant version of DNA gyrase (gyrA462) that is resistant to the toxic effects of ...induces efficient ATP-dependent DNA cleavage by gyrase.” J Mol Biol. 1993 Dec 5;234(3):534-41. PubMed ...

...resistance, mediated by a single base pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for resistant...

...phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene; Promega e14-(McrA-) recA1 endA1 gyrA96 thi-1 hsdR17(rK...endA1 nupG tonA::Ptrc ccdA DB3.1 Invitrogen F- gyrA462 endA Delta(sr1-recA) mcrB mrr hsdS20 (rB- mB-) ...