We narrowed to 73 results for: GYRA
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Plasmid#216229PurposeS. aureus USA300 GyrA Codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ub-GyrA
Plasmid#128737PurposeExpresses Ub fused to GyrA intein at the C-terminalDepositorInsertUBC
TagsMxe GyrAExpressionBacterialPromoterT7Available SinceSept. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
GB1-GyrA
Plasmid#128738PurposeExpresses GB1 fused to GyrA intein at the C-terminalDepositorInsertGB1
TagsGyrAExpressionBacterialPromoterT7Available SinceAug. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
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INT_Cargo_PgyrA_gfp
Plasmid#212138PurposeCargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
ExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_PgyrA_katG_dadA6
Plasmid#212142PurposeCargo plasmid for integrating PgyrA expression cassette of catalase-peroxidase and D-amino acid dehydrogenase into the genome. Plasmid can be removed by incubating cells at 37 C.DepositorInsertcatalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
ExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA_F164R238
Plasmid#216230PurposeS. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialMutationChanged arginine 238 to serine, phenylalanine 168…PromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CfaC-Gyra
Plasmid#122950PurposeBacterial expression of C-inteinDepositorInsertCfaC
ExpressionBacterialAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEC2861 (pSpy1C-PgyrA_ffluc)
Plasmid#218511Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseMutationdeletion of Plac_mrfp cassettePromoterPgyrAAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
C-Terminal Split Cas9 with GyrA intein
Plasmid#58694PurposeExpresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
UseAAV and CRISPRTagsGyrA Csplit Intein and NLSExpressionMammalianPromoterCBhAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
N-Terminal Split Cas9 with GyrA intein
Plasmid#58693PurposeExpresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
UseAAV and CRISPRTags3xFlag, GyrA Nsplit Intein, and NLSExpressionMammalianPromoterCBhAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-His6-IntCtr-GyrA-His6
Plasmid#126019PurposeBacterial expression of IntCtr-GyrA fusion protein (with N- and C-terminal His6)DepositorInsertHis6-IntCtr-GyrA-His6
Tags6xHis and GyrA-6xHisExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
N-Terminal Split Cas9 D10A Nickase with GyrA intein
Plasmid#58695PurposeExpresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase productionDepositorInsertD10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
UseAAV and CRISPRTagsGyrA Nsplit Intein, HA Tag, and NLSExpressionMammalianMutationD10A nickase converting mutation to SpCas9PromoterCBhAvailable SinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
S17-1λpir gyrAR462C
Bacterial Strain#237425PurposeThis engineered E. coli S17-1 λpir strain, featuring a mutation in the gyrA gene (462Arg→Cys), was designed to confer resistance to the CcdB toxin, allowing it to survive with a ccdB-carrying plasmid.DepositorBacterial ResistanceNoneAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTXG
Plasmid#52280PurposeBacterial expression of TARGET proteins C-terminally fused to GyrA intein and GSTDepositorTypeEmpty backboneTagsGyrA-intein-GSTExpressionBacterialPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXG-hTDG
Plasmid#52281PurposeBacterial expression of human TDG C-terminally fused to GyrA intein and GSTDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-hTDG-IntE2
Plasmid#52270PurposeBacterial expression of human TDG C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXG-hXRCC1
Plasmid#52283PurposeBacterial expression of human XRCC1 C-terminally fused to GyrA intein and GSTDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTXG-hTDG-K330A
Plasmid#52282PurposeBacterial expression of human TDG-K330A C-terminally fused to GyrA intein and GSTDepositorInserthTDG (TDG Human)
TagsGyrA-intein-GSTExpressionBacterialMutationK330A, SUMO acceptor site mutationPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only