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We narrowed to 20 results for: Gateway destination

Showing: 1 - 20 of 20 results

  1. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ... the Gateway cloning method and its advantages. An introduction to Gateway technology The Gateway cloning...With the appropriate entry and destination vectors, one can use Gateway to clone a gene of interest into...one Gateway vector to produce the desired expression clone. This is possible thanks to the Gateway vectors...Systems (MuLE) Kit, the MultiSite Gateway cloning kit, and MultiSite Gateway plasmids. Constant reading frame...links below to find Gateway plasmids for your organisms of interest: Find Gateway cloning plasmids here... needs. Since its invention in the late 1990s, Gateway cloning technology has become very popular as a...attB sites creates attL and attR sites.   Gateway vectors contain modified versions of the att sites...

  2. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...and do not appear in the final construct. The destination vector contains sites with complementary overhangs...ligation can be done in one 30-minute reaction. The destination vector and entry vector(s) are placed in a single...IIS enzyme and ligase. Although the original destination vector + insert may spontaneously religate, this...can be assembled with Golden Gate. The popular Gateway cloning system produces constructs with an attB... to efficiently clone up to 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages...inactivation step. If your genes of interest or destination vector contain multiple internal restriction ...want to consider using an alternative method like Gateway cloning or Gibson assembly. Another important consideration...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...ligated into Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt...ordered assembly of multiple components into a destination vector. The first step in CRISPR/Cas9 Golden ...together to allow for ordered assembly into a destination vector that expresses Cas9. This is illustrated...drive ordered assembly into a Cas9-containing destination vector. Note: depending on which method you use...transcriptional activator)-containing plasmids. Each destination vector contains GFP, enabling you to select cells...express up to 7 gRNAs. The kit contains different destination vectors depending on the total number of gRNAs...most gRNA is cloned into the Cas9-containing destination vector. These constructs are digested using BsaI...

  4. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...toxin CcdB. Gateway cloning takes advantage of this property by including ccdB on its destination plasmids...plasmids. In the destination plasmid, the ccdB gene is flanked by recombination sites. To introduce a gene of... of interest into the destination plasmid, recombination sites must first be added to flank the gene. ...

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...collection page Yeast GAL4, PGK, ADH1, ADE2, TRP1 Gateway destination vectors - Inducible expression...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST ...N-terminal GST pGCS-N5(GST) - GST tagged Gateway destination vector for mammalian, avian, xenopus, or ... genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression pLenti CMV/TO ...TO Zeo DEST - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones...GFP-Zeo DEST (719-1) - 3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-... Tet-inducible lentiviral Gateway destination vector for gene expression...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...components into a destination vector in a predefined order. Finally, the destination vectors are used ...be used for each Multisite Gateway compatible destination vector for a maximum of 24 elements assembled...component plasmids from building blocks via Golden GATEway Cloning Frustrated by the complexity of assembling...combining the advantages of Golden Gate and Multisite Gateway cloning methods developed by the lab of Joachim...just the set of tools that you need. The Golden GATEway cloning kit simplifies the cloning process for ...used with Multisite Gateway cloning to generate the final construct. Up to eight entry vectors can be ...transgenesis construct. Plasmids constructed using Golden GATEway cloning have been utilized to create recombination...

  7. Lentivirus Plasmids

    Type
    Collection
    ...Linzhao Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible expression...Iain Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression. Miguel Ramalho-Santos...expression, TRE-gateway, with puromycin selection. See Root Lab pLEX and pLIX Gateway backbones for additional... Tet-based inducible shRNA or cDNA expression, gateway plasmid, and constitutive hygromycin selection....pULTRA versions . Malcolm Moore 25895 pLX301 3rd Gateway plasmid for CMV driven expression of cDNA. See ...

  8. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is a customizable...an adapted destination vector with a heterologous promoter outside of the Multisite Gateway® cassette,... systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry clones to be used...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate ... phage integrase system which is the basis for Gateway cloning. The combination of all 11 att pairs in...customizable Gateway®-based toolkit that allows scientists to generate fusion proteins from any ORF available...as a standard entry clone. ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned into pDONR221...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2...74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...your gene of interest and cloned into provided destination vectors using low-cost, one-pot golden gate cloning...The collection is compatible with both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform...PMID: 26854857   Examples of FusX-Compatible Destination Vectors Available at Addgene: Lab Plasmids...mammalian systems. The DULIP system is comprised of Gateway-compatible plasmids in which “bait” proteins are...

  10. Synthetic Biology - Overview

    Type
    Collection
    ...Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium...

  11. Zebrafish Plasmid Collection

    Type
    Collection
    ...Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino- or ...HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders ...

  13. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pMH-Halo tag - Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/C-Halo...- Mammalian Expression pENTR4-SNAPf (w878-1) - Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf - Xenopus...- Mammalian Expression pENTR4-CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag - Bacterial...

  15. Molecular Cloning Techniques

    Type
    Guide
    ...completed via Gateway cloning. Addgene's collection contains thousands of Gateway donor , Gateway entry , and...backbone. Created with BioRender.com. Gateway Recombination Cloning Gateway cloning is a recombination-based... be rapidly shuttled into any compatible Gateway destination vector, which contains attR sites, via LR...move it into any destination plasmid that fits your experimental goal. Although Gateway cloning is a simple... and Gateway destination vectors with different promoters, tags, selection markers, and fluorescent proteins...conditions. Read more in our Gateway Cloning blog post . Figure 2: Summary of Gateway cloning. Created with ...Molecular Cloning Techniques Restriction Enzyme Gateway TOPO Gibson Golden Gate & MoClo Ligation Independent...

  16. Modular Cloning Guide

    Type
    Guide
    ...chloroplast-specific genetic modules, including destination vectors specific for tobacco ( N. tabacum ) or...nonfunctional "dummy" parts, 34 backbones, and 6 destination vectors for use in E. coli for a variety of applications...Takashi Yamamoto Nine pFUS array vectors and destination vectors designed for use with the Golden Gate...inserts in one cloning step rather than two. Golden GATEway Cloning Kit Other, Zebrafish Expression Joachim...toolkit that combines Golden Gate and Multisite Gateway cloning. This system was specifically designed ...

  17. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen...including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction...yield of DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant...

  18. Adenovirus Guide

    Type
    Guide
    ... with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen...including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction...yield of DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant...

  19. Lentiviral Vector Guide

    Type
    Guide
    ... with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen...including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction...yield of DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant...

  20. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... with certain cloning methods (such as a Gateway destination vector), so be sure to confirm your chosen...including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction...yield of DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant...
Showing: 1 - 20 of 20 results