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We narrowed to 19 results for: Karl Deisseroth

Showing: 1 - 19 of 19 results
  1. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...Optogenetics Karl Deisseroth New serotype pAAV-hSyn-hChR2(H134R)-EYFP AAV2 Optogenetics Karl Deisseroth New ...serotype pAAV-Ef1a-DIO mScarlet AAV5 Controls Karl Deisseroth New serotype pAAV_hSyn-PdCO-mScarlet-WPRE...
  2. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...pAAV-Ef1a-DIO EYFP Karl Deisseroth 11447 AAV5 pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2 pOTTC1032...Bryan Roth 114472 AAVrg pAAV-hSyn-mCherry Karl Deisseroth 83895 AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 ...
  3. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...Stanford University, led by neurobiologist Karl Deisseroth, combined optical and genetic methods to photo-stimulate...30503173. PubMed Central PMCID: PMC6317997. Deisseroth, Karl, et al. "Next-generation optical technologies...17035522. Pubmed Central PMCID: PMC2820367. Deisseroth, Karl. "Optogenetics." Nature methods 8.1 (2011)... PMC3979449. Fenno, Lief, Ofer Yizhar, and Karl Deisseroth. "The development and application of optogenetics...optogenetics” was coined (Boyden et al., 2005; Deisseroth et al., 2006). Despite its relatively recent ...pharmacological or electrophysiological methods (Deisseroth, 2011; Mei and Zhang, 2012; Towne et al., 2016...
  4. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...pAAV-Ef1a-fDIO EYFP AAV5. AAV8, AAVrg Control Karl Deisseroth New serotype (multiple) rAAV-CAG::FLEX-rev...pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE...
  5. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...-fDIO hChR2(H134R)-EYFP AAVrg Optogenetics Karl Deisseroth New viral tool AiP13987: pAAV-AiE0452h_3xC2...
  6. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...elsewhere (here and here for instance) [2], [3]. Karl Deisseroth of Stanford University , one of the key scientists...the kinetics of opsin activity/inactivity. Dr. Deisseroth has also generously made many of his optogenetic...various universities as well as Addgene. See the Deisseroth lab homepage for details about types of constructs...using stereotaxic surgery (see videos below). The Deisseroth lab has developed many of these AAV constructs... the expression of your opsin. Thankfully the Deisseroth constructs coexpress a fluorescent protein, which...Twitter @derekpsimonphd.    References 1. Tye KM, Deisseroth K. Optogenetic investigation of neural circuits...
  7. Optogenetics AAV Preps

    Type
    Collection
    ..., 5 Karl Deisseroth 137151 pAAV-nEF-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Constitutive 8 Karl Deisseroth 137152...dependent 8 Karl Deisseroth 137144 pAAV-nEF-Coff/Fon-ChR2-mCherry nEF ChR2 mCherry Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 26968 pAAV-Ef1a-DIO ChETA-EYFP EF1a ChETA EYFP Cre dependent 1, 5, 9 Karl Deisseroth...rg* Karl Deisseroth 26971 pAAV-CaMKIIa-eNpHR 3.0-EYFP CaMKII eNpHR 3.0 EYFP Constitutive 1, 9 Karl Deisseroth...dependent 8 Karl Deisseroth 137154 pAAV-nEF-Coff/Fon-NpHR3.3-EYFP nEF NpHR 3.3 EYFP Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137156 pAAV-nEF-Con/Foff 2.0-iC++-EYFP nEF iC++ EYFP Cre dependent 8 Karl Deisseroth...mCherry nEF ChR2 mCherry Cre and Flp dependent 8 Karl Deisseroth 137143 pAAV-nEF-Con/Foff 2.0-ChR2-mCherry nEF...
  8. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Optogenetics Karl Deisseroth AV-1-26968P 26968-AAV1 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics Karl Deisseroth AV-1...Optogenetics Karl Deisseroth AV-9-26968P 26968-AAV9 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics Karl Deisseroth AV-9...EYFP Karl Deisseroth AV-1-35504 35504-AAV1 pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry Karl Deisseroth AV-...)-EYFP Karl Deisseroth AV-1-35506 35506-AAV1 pAAV-CaMKIIa-hChR2(E123A)-mCherry Karl Deisseroth AV-1-35512...eYFP.WPRE.hGH Karl Deisseroth AV-2-35505 35505-AAV2 pAAV-CaMKIIa-hChR2(E123A)-EYFP Karl Deisseroth AV-2-PV0102...D156A)-EYFP Karl Deisseroth AV-5-35505 35505-AAV5 pAAV-CaMKIIa-hChR2(E123A)-EYFP Karl Deisseroth AV-5-PV0105...D156A)-EYFP Karl Deisseroth AV-1-26972P 26972-AAV1 pAAV-hSyn-eNpHR 3.0-EYFP Karl Deisseroth AV-8-PV3706...
  9. Control AAV Preps

    Type
    Collection
    ...Constitutive 8 Karl Deisseroth 131001 pAAV-hSyn-mScarlet Syn mScarlet Constitutive 1, 8 Karl Deisseroth 135630...dependent 8 Karl Deisseroth 137162 pAAV-Ef1a-Con/Foff 2.0-EYFP EF1a EYFP Cre dependent 8 Karl Deisseroth 98927...dependent 8 Karl Deisseroth 137134 pAAV-Ef1a-Coff/Fon-mCherry EF1a mCherry Flp dependent 8 Karl Deisseroth 137138...PHP.eB Karl Deisseroth 114472 pAAV-hSyn-mCherry hSyn mCherry Constitutive 1, 2, 5, 8, 9, rg* Karl Deisseroth...dependent 9 Karl Deisseroth 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent 1, 2, 5, 9, rg* Karl Deisseroth...dependent 8 Karl Deisseroth 137133 pAAV-Ef1a-Con/Foff 2.0-mCherry EF1a mCherry Cre dependent 8 Karl Deisseroth..., rg* Karl Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP Flp dependent 1, 2, 5, 8, 9, rg* Karl Deisseroth...
  10. Retrograde AAV viral preps

    Type
    Collection
    ...mCherry Control Karl Deisseroth 114469 pAAV-CaMKIIa-mCherry CamKII mCherry Control Karl Deisseroth 50459 pAAV-hSyn-DIO-mCherry...Control Karl Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, Flp-dependent Control Karl Deisseroth 114470...Recombinases Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Karl Deisseroth 55637 pAAV-EF1a-Flpo...Recombinases Karl Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases Karl Deisseroth 87306 AAV...Optogenetics Karl Deisseroth 26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn Activator Optogenetics Karl Deisseroth 28017...Control Karl Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent Control Karl Deisseroth...Optogenetics Karl Deisseroth 55639 pAAV-Ef1a-fDIO hChR2(H134R)-EYFP EF1a Activator Optogenetics Karl Deisseroth...
  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...tissue clearing (13,14). A few years later, Karl Deisseroth’s lab developed CLARITY, which is a hydrogel-based...332-337. Pubmed. R. Tomer, L. Ye, B. Hsueh, K. Deisseroth. (2014) Nat Protoc., 9, 1682-1697. Pubmed. C....
  12. Recombinases AAV Preps

    Type
    Collection
    ...fusion tag) 8, rg* Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a none 11, rg* Karl Deisseroth 121675 pAAV-EF1a-fDIO-Cre...fusion tag) 1, rg* Karl Deisseroth 55637 pAAV-EF1a-Flpo EF1a none 1, 2, rg* Karl Deisseroth 87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA...Tools 183535 pAAV-CaMKIIa-Flpo CaMKIIa none 8 Karl Deisseroth 236288 pAAV.TBG.PI.FLPo.rBG TBG none 8 Melina...) PI 55638 pAAV-EF1a-vCre EF1a none 8, rg* Karl Deisseroth *AAVrg = retrograde serotype, produced with...
  13. Biosensor AAV Preps

    Type
    Collection
    ...5, 8 Karl Deisseroth 137123 pAAV-Ef1a-Con/Foff 2.0-GCaMP6F EF1a GCaMP6f none Cre dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137120 pAAV-Ef1a-Con/Foff 2.0-GCaMP6M EF1a GCaMP6m none Cre dependent 8 Karl Deisseroth...Constitutive 8 Karl Deisseroth 137126 pAAV-Ef1a-Con/Fon-sRGECO Ef1a sRGECO none Cre and Flp dependent 8 Karl Deisseroth...dependent 8 Karl Deisseroth 137128 pAAV-Ef1a-Coff/Fon-sRGECO Ef1a sRGECO none Flp dependent 8 Karl Deisseroth...-GCaMP6F EF1a GCaMP6f none Flp dependent 8 Karl Deisseroth 158757 AAV-CAG-SomaGCaMP6f2 CAG soma-GCaMP6f2...6m-p2A-ChRmine-Kv2.1-WPRE Syn GCaMP6m none Constitutive 8 Karl Deisseroth 137119 pAAV-EF1a-Con/Fon-GCaMP6M EF1a GCaMP6m...-GCaMP6M EF1a GCaMP6m none Flp dependent 8 Karl Deisseroth 137165 pAAV-Ef1a-Con/Fon/Von GCaMP 6m EF1a ...
  14. Viral Production at Addgene

    Type
    Blog Post
    ...pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG...
  15. Chemogenetics AAV Preps

    Type
    Collection
    ...Inhibition mCherry Cre and Flp-dependent 8 Karl Deisseroth 177673 pAAV-nEF-Con/Foff DREADD Gi-mCherry ...rM4D(Gi) - Inhibition mCherry Cre-dependent 8 Karl Deisseroth 135635 pAAV-S5E2-Gq-P2A-dTomato hM3D(Gq) - ...
  16. Viral Production

    Type
    Collection
    ...pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG-FLEX-rc...
  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    .... 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of.... 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Mitochondrial...
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