Technologies Enabled by NanoLucĀ® Luciferase
Type
Blog Post
...consists of NLuc fused to enhanced green fluorescent protein (GpNluc) and the other consists of NLuc fused ... two great applications of the NLuc technology. The first couples NLuc to fluorescent proteins to make...imaging.
The solution is to couple Nluc with a red-shifted fluor. Here, NLuc internally excites the fluor through...reporter by linking two CyOFP1 molecules to NLuc (CyOFP1-NLuc-CyOFP1; Antares). Direct injection of plasmids...fluorescent protein fusion (OgNLuc). Both fluorescent proteins can be excited by Nluc emission and the lab has...shrimp to a compact powerhouse of bioluminescence, NLuc is 100-fold brighter than our more common luciferases...reaction but for how sensitive you can make a reaction. NLuc requires 100-fold less protein to produce the same...