...transcription: NP, VP35, VP30, and L (reviewed in 5). The RNA genome is encapsidated by the nucleoprotein, NP. NP...viral mRNAs are translated) by cellular machinery. NP encapsidates the negative sense, single-stranded ...important that all of these plasmids (those containing NP, VP35, VP30, L, and the minigenome) are efficiently...For example, particular ratios of plasmids encoding NP and VP35 allow for efficient polymerase activity,...with appropriate plasmid ratios, we suggest: 500 ng NP, 500 ng VP35, 100 ng VP30, 100 ng L, and 2 μg minigenome...

...sharing landscape as well.     References Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, ...

...10.1038/mtna.2015.32 Maynard LH, Smith O, Tilmans NP, Tham E, Hosseinzadeh S, Tan W, Leenay R, May AP,...

...recombinant antibody plasmid collection References Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, ...

...Addgene.   Find anti-GFP [N86/8R] here!   Andrews NP,  et. al. A toolbox of IgG subclass-switched recombinant...

...yet. References and Resources References Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, ...

...non-denaturing buffer containing non-ionic detergents, such as NP-40 or triton-X, to preserve native protein conformations...

...Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents...

...Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents...

....4.1406. PMID: 4884687; PMCID: PMC225270. Higgins NP, Vologodskii AV. Topological Behavior of Plasmid ...

...Protocols (Link opens in a new window) References Andrews NP, et al. 2019. A toolbox of IgG subclass-switched ...