Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
Type
Blog Post
...transcription: NP, VP35, VP30, and L (reviewed in 5). The RNA genome is encapsidated by the nucleoprotein, NP. NP...viral mRNAs are translated) by cellular machinery. NP encapsidates the negative sense, single-stranded ...important that all of these plasmids (those containing NP, VP35, VP30, L, and the minigenome) are efficiently...For example, particular ratios of plasmids encoding NP and VP35 allow for efficient polymerase activity,...with appropriate plasmid ratios, we suggest: 500 ng NP, 500 ng VP35, 100 ng VP30, 100 ng L, and 2 μg minigenome...