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We narrowed to 66 results for: OAT

Showing: 1 - 20 of 66 results

  1. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...is a light-oxygen-voltage sensing domain from the oat plant (Avena sativa). In darkness, the AsLOV2 domain... team used nickel-coated agarose beads coated with his-tagged OptoNBs. OptoNB coated beads were then imaged...instead use OptoNBs. You fill a column with OptoNB coated beads and wrap the column with blue LED lights....

  2. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Clathrin-coated vesicles Clathrin light chain GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles...Clathrin-coated vesicles Clathrin miRFP703 Vladislav Verkhusha 21741 Clathrin-LCa-EYFP Clathrin-coated vesicles...Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-Clc Clathrin-coated vesicles Clathrin, light polypeptide mRFP Ari Helenius...

  3. AAV for Neuronal Tracing

    Type
    Collection
    ...which is supplied in trans . (3) Nascent G-protein coated RAVB is released from the starter cell and free...to the starter cell are infected with G-protein coated RABV. These projection neurons do not express G-protein...G-protein, and thus do not give rise to G-protein coated RAVB. Resources Addgene Blog: Rabies and Neuronal...

  4. Bacterial Expression Systems

    Type
    Collection
    ... super resolution microscopy. pZE31ms2 25860 MS2 coat protein binding sites Philippe Cluzel RNA localization...within this plasmid. pZE31-DsRed-ms2x2 26248 MS2 coat protein binding sites Philippe Cluzel RNA localization...

  5. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ... directly or by binding to an antibody that is coating the bottom of the well), and then its concentration... Sandwich and competitive ELISA use antibodies coating the wells to capture antigen from a sample, whereas... sandwich ELISA. This technique works by first coating the wells of a flat bottomed plate with a capture...kit is ordered, the wells are typically already coated with the antibody. Then, you add your sample to...recognizes it, while the bottom of the wells are coated with another protein — known as a reference antigen...to the wells of a plate instead of an antibody. Coating the wells with a purified protein allows you to...an antibody that recognizes that protein, while coating the wells with an experimental sample allows for...

  6. Antibody Guide

    Type
    Collection
    ...primary conjugated antibody. Overview of application Coat antigens to the well of a plate, either by charge...] [primary source secondary recognizes], e.g. , “goat anti-rabbit.” Choose a secondary that recognizes...

  7. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...ELISAs follow the same overall process. First users coat a multiwell plate with a capture reagent. The capture...reporter enzyme conjugated antibody binds to antigen coated on the multiwell plate. In an indirect ELISA a ...In a sandwich ELISA the multiwell plate is first coated with a capture antibody that binds to the antigen...used. In a competitive ELISA a multiwell plate is coated with a competing antigen (green).  The sample (...common type of ELISA, the sandwich ELISA, users first coat a multiwell plate with a capture antibody and then...setup of the method can vary, typically users first coat the multiwell plate with a reference antigen and...

  8. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...parkinsonsdisease A post shared by Ladies in labcoats (@ladiesinlabcoats) on Dec 19, 2018 at 4:36am PST Buddy..., 2018 Eppi the red nosed reindeer from @ladiesinlabcoats Many teams recycled lab materials into holiday...up about sustainability in the lab!). The @ladiesinlabcoats used a pipette tip box and microcentrifuge...love your Insta!! #LILCChristmas . . #lab #ladiesinlabcoats #Christmas #labchristmas #cute #reindeer #...

  9. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...of the PP7 and MS2 coat protein binding sites to tether many copies of the FP-coat protein fusions to ... the ribosome, TRICK (Translating RNA Imaging by Coat protein Knock-off) instead takes advantage of the...to the coding region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) an RFP protein that binds ...binds to the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...

  10. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...Mouse IgG2a) Goat anti-mouse IgG2a CASPR anti-CASPR mAb K65/35 (Mouse IgG1) Goat anti-mouse IgG1... are conjugated with the same dye (say, you have Goat Anti-Rabbit IgG and Donkey Anti-Mouse IgG both with...channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG   If you need more signal, another...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... 6-12 phage PP7 coat protein binding sites and 24 copies of the MS2 bacteriophage coat protein binding...cleverly termed TRICK, for Translation RNA Imaging by Coat protein Knock-off, is made up of three components...to the coding region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to... to the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...binding sites to tether many copies of the FP-coat protein fusions to their reporter mRNAs. Using the TRICK...

  12. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... application, using a Goat anti-mouse IgG2a cross-adsorbed secondary and a Goat anti-rat IgG1cross-adsorbed...cross react with other species. For example, some goat anti-mouse secondary antibodies recognize some rat...

  13. Common Injection Routes in Mice

    Type
    Blog Post
    ...which prevents accidental tears or punctures to the throat. The feeding tube can be flexible or metal, depending... learning curve! Restraint: scruffing Location: throat Gauge (bulb-tipped feeding tube): 18–20 Volume:... n/a < 0.05 mL Beginner Oral gavage Scruffing Throat 18–20 (bulb tipped) up to 10 mL/kg Intermediate...

  14. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ..., a popular stabilizing modification is 3’ phosphorothioate linkage, where a sulfur replaces a nonbridging...coupling several of them together, for example phosphorothioates with 2’-O-methyl modifications, proved more...

  15. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...questions: “who moved my goat anti-rabbit?!”*  *a secondary antibody generated in goat against rabbit immunoglobulins...

  16. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...viral handling. Gloves are always required and lab coats are either recommended or required in BSL-1 and ...require or recommend disposable gowns over your lab coats and double gloving, so that, if needed, a layer ...

  17. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...protein called rabies glycoprotein (G). G-protein-coated RABV infects cells via the G receptor. In neurons...envelope (but not the viral genome). Nascent G protein-coated RABV is then released from the starter cell and...

  19. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...active swimming in attempts to escape, or passive floating on the water surface. The latter reflects a state...of time spent actively swimming versus passive floating. In the same paper, the authors used lentivirus...

  20. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of biotinylated...N206A/8R] antibody here!   Figure 5: Free-floating rat brain sections stained with Anti-GFAP. The...
Showing: 1 - 20 of 66 results