CRISPR Guide
Type
Guide
...inactive dCas9 fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...purification. Various epitope tags including 3xFLAG-tag, PA, and biotin tags, can be used for enChIP, as well... B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. Nature . 520(7546):186-91. PMID: 25830891...