FlipGFP, a novel fluorescence protease reporter to study apoptosis
Type
Blog Post
...flipping a single β-strand of GFP, hence the name FlipGFP. FlipGFP increases fluorescence 100-fold compared to...in vivo using the FlipGFP-based caspase reporter
The Shu Lab successfully used FlipGFP to study apoptosis...from UCSF designed an improved apoptosis reporter, ZipGFP, which utilized the self-assembling split GFP (...other reporters (Zhang et al., 2019).
How does FlipGFP work?
GFP contains 11 β-strands and one central...antiparallel. To prevent binding, the β strands in FlipGFP were redesigned so that they are parallel to each...reporter based on superfolder Cherry (sfCherry). Like FlipGFP, sfCherry was split into β1-9 and β10-11, where...provides a proof of concept that the designed used in FlipGFP can be applied to other fluorescent proteins to...