...Our previous blog post The PAM Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity...toolkit available to researchers will continue to expand. Final thoughts Since the first use of Cas9 to ..., et al. "Engineered CRISPR-Cas9 nuclease with expanded targeting space." Science 361.6408 (2018): 1259...

...hundreds of scientists about various ways we might expand Addgene’s services. There was an overwhelmingly... to facilitate the formation of partnerships to expand the service and figure out what we can afford to...educational content via the Addgene Blog and our expanding website resources. I hope this gave you the impression...

...smartphone that can now run several more apps. The expanded functions of this “CRISPR 2.0” include gene regulation...multiple functions using a single dCas9 and an expandable library of PUF-effector modules. The components...will be available at Addgene soon. We will also expand the collection and a database of orthogonal Casilio...

...).This toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst...Podcast Segment    Photocleavable protein PhoCl expands optogenetics toolbox Increasingly popular Optogenetics...proteins (FP) for cellular imaging is constantly expanding. Case in point - check out the growing list of...new base editors with distinct PAM sequences, expanding the number of available target sites for base ...

...enzyme. Fast facts – PAM requirement: NGG and an expanding number of others. Best for: a wide range of DNA...scientist toolbox. If your genome engineering goals expand beyond generating frameshift mutations or single...my CRISPR experiment? The PAM requirement and expanding CRISPR beyond spCas9 Cas proteins More resources...

...see CBE-3 in Figure 3). Plant CBE and ABE with expanded PAM compatibility All of the base editors mentioned.... Hua, Kai, Xiaoping Tao, and Jian‐Kang Zhu. "Expanding the base editing scope in rice by using Cas9 variants.... PubMed PMID: 29867128. 11. Li, Chao, et al. "Expanded base editing in rice and wheat using a Cas9-adenosine...

...below). Expand: Collaboration opportunities outside an organization The next step is to expand your efforts... to start close to your comfort zone and slowly expand from there. Start small: Collaboration opportunities...

...modified through phosphorylation (1). However, the expansion of studies on protein phosphorylation in an array...PubMed PMID: 15790858. 5. Park, Hee-Sung, et al. "Expanding the genetic code of Escherichia coli with phosphoserine... Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013):...

... of more stable, brighter, and specific dyes, expanding the capabilities of fluorescence-based techniques...® system, the new Janelia Fluor HaloTag Ligands expand the self-labeling capabilities of this platform...Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist toolbox via new far-red-emitting...

...made improvements to base editors including: expanding targeting scope improving editing efficiency decreasing...new base editors with distinct PAM sequences, expanding the number of available target sites for base ... variants, BE3 editing window variants Greatly expands the number of target loci for base editing Rees...

...called hybridomas, are individually isolated and expanded. The clonal cells are screened to identify hybridoma... Addgene's First 10 Years Data Freedom: The Expansion of Data Sharing in Research Publications ...

...information sharing, and research reproducibility while expanding our suite of materials and services to support...many exciting things planned in 2023, from the expansion of our Data Hub to process improvements in every...

...year ago, Sacher and Zheng found that they could expand their role in the phage community by being a repository...Sacher says. Sacher hopes that as Phage Directory expands, it can also fill this role by cataloging and archiving...

...inviting serendipity into your research lies in expanding the scope of your exploration. While it is essential...diverse literature. By delving into new topics and expanding your knowledge base extensively, you open doors...

...or protein sequences to sequences in a database ExPASy translation tool: Translates a DNA sequence using...for fluorescent proteins and their properties.  Expasy: Bioinformatics resource portal which includes ...

...single base-editors has resulted in the rapid expansion of the genome editing toolkit. However, a challenge...quantitative editing assay. To help accelerate the expansion of genome editing tools, the Reuben Harris lab...

...Participating in activities outside of the lab expands your professional network. No brainer! Engaging...spent away from the bench, but as an opportunity to expand associations outside of the traditional academic...

...neuroscience reagents and data, the platform can be expanded for any recombinant antibody tool and therefore...distributions to cover costs, which can limit expansion into new services. “We appreciate NIH’s vision...

...applications such as super resolution, electron, and expansion microscopy. hfYFP is not only brilliantly bright...than most FPs. The group also tested hfYFP in expansion microscopy as well as correlative light and electron...

...AAV inventory for the entire collection! We are expanding our entire control AAV inventory to give scientists...Overview of AAV Encoded Calcium Sensors). We are expanding the inventory of these vectors to more serotypes...