We narrowed to 36 results for: aacs
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TypeBlog Post...stop cassette following recombination. Image from Isaacman-Beck et al., 2019. Sparse Predictive Activity...GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck et al., 2019. Applications for SPARC SPARC...Methods 9:47–55 . https://doi.org/10.1038/nmeth.1800 Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher...
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Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...lobe. Image from Isaacman-Beck et al., 2019. Get started with sparse labeling Isaacman-Beck et al., bioRxiv... -
Molecular Biology Reference
TypeGuide...CGU, CGC, CGA, CGG, AGA, AGG Asparagine Asn N AAU, AAC Aspartic acid Asp D GAU, GAC Cysteine Cys C UGU, ... -
What's New in CRISPR - Spring 2019
TypeBlog Post...nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal DNA cleavage at 48 °C, a temperature... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...339 (6121): 819–23. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. ... -
Components of CRISPR/Cas9
TypeBlog Post...ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC The synthetic guide RNA or gRNA (sometimes ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...methylase adds a methyl group to the adenine in AACNNNNNNGTGC or GCACNNNNNNGTT Impact on cloning and digests... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...step-function artificial anion conducting channelrhodopsin (aACR). It is activated by cyan light and exhibits an ... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog Post...19411448. PubMed Central PMCID: PMC2684168. Witz, Isaac P. "The tumor microenvironment: the making of a ... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...; Reverse oligo: 5’- AATTGXXXXXXXXGAATAGGTCTTCTCGAAGACCCAAACNNNNNNNNNNNNNNNNNNNC -3’, where NNN and XXX... -
Plasmids 101: Cre-lox
TypeBlog Post...directionality, and the canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. The loxP sequence does... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM (2015) Highly efficient... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...thermophilus (St) NNAGAAW Treponema denticola (Td) NAAAAC ~20 additional Cas9 species PAM sequence ... -
CRISPR Activation: A Practical Guide
TypeBlog Post...JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM (2015) Highly efficient... -
March for Science
TypeBlog Post...Hays. Stephanie would like to additionaly thank Isaac Plant for thoughtful edits. Stephanie Hays recently... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post...Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios X, Huang P-Y, ... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...circuit design automation." Science352.6281 (2016): aac7341. PubMed PMID: 27034378. 4. Lou, Chunbo, et al. ... -
A History of Genome Engineering in Popular Culture
TypeBlog Post..., SciFi saw the rise of literary giants such as Isaac Asimov, Arthur C. Clarke, James Blish, and John ... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...N-3'3'-CN...NCAAA-5'You need to add the CACC and AAAC cloning overhang. Q3: When designing oligos for ... -
TALENs for Endogenous Zebrafish Genes
TypeCollection...abcg2a TAL3400 & TAL3401 TGTCCTCATCCCCGTCCCgccgcggcgccaccgtCAGCTTCCACAACATCAA adam15 TAL3016 & TAL3017...ahcyl1 TAL3018 & TAL3019 TGACAGACTGTGGCGAGGatgccaagctggaggcGACGAAACAGGCCAGCAA amh TAL3210 & TAL3211 TCCAGGCAAGATTTGGGCtgatgctgatgatgacCGTGGCGATTGGGTCGTA...Aminopeptidase-o TAL3020 & TAL3021 TGATCTGGATCCTAACAAggatgacttgcccctgtGTGCGAACACCAACCATA arf6 TAL3212 & TAL3213 TGGGGAAGATGCTTTCCAagatcttcggcaacaaGGAGATGAGAATATTGA... TGGGGAAGATGCTTTCCAagatcttcggcaacaaGGAGATGAGAATATTGA arnt1 TAL3214 & TAL3215 TCCCGTCTCTGGCCATGAcctcctctaatggatcTCATTCCAACGGTGTGCA... apoa4 TAL3022 & TAL3025 TGGCAGGACCAACCAATGcccagcatggacctggTGAAAAATGCTTTCTGGA apoa4-like TAL3023 & TAL3024...TAL3024 TGAAGGTTCTTGTGGTGCtcacacttgctgtggtTACAGGTAAGAAACTAAA apoeb TAL3402 & TAL3403 TGCCAGGCTCGTAGCCTGttccaggctgatgccccTCAGCCCAGATGGGAGGA...arap3a TAL3026 & TAL3027 TGGCAACAATCGGTCCGTgctcagatgcagaggaCCTGTTGGCTTCCATCCA ARSA TAL 3002 & TAL 3003...