...mM Sodium Citrate, pH 7.0 0.5% (wt/vol) N-laurosylsarcosine (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol®...

...both the design and functionality of searches. A better way to search First, check out the new Addgene homepage... it, we’ll search for the calcium biosensor, GCaMP, and click through to the plasmid search results: ...specific search terms used, so you will only be shown filters relevant to your current search. We’ll take...dropdown list: The search bar will suggest direct links to other types of searches including depositing...plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched search engine...created can narrow your search results to exactly what you need: Better searching with filters Not every...isn’t new to Addgene’s plasmid search. The new design of the plasmid search page moves the filters from ...

...the opinions or research on each topic. Resources for marching and advocacy If the march for science rallies...the March for Science has the details for the march in Washington as well as the satellite marches. Anyone...the March is not about Democrats or Republicans (16). This is a Pro-Science and Pro-Evidence march. Who...Who is marching and why? Who? All sorts of people are choosing to march on April 22nd. As of April 13th...satellite marches are scheduled to take place around the world. There is no requirement that marchers or supporters...scientists and non-scientists alike are marching for. I will be marching to advocate for science education ...modern research, more funding is required. This is by no means an exhaustive list and every marcher is not...

...Uncovering Ancestral Bias in CRISPR Research: We spoke to researchers from the Boehm and Beroukhim labs ...Week, we're highlighting eight amazing posts on research done in our depositor labs. Some are written by...AAV Q&A with Tim Miles: While not technically a research story, I couldn't resist adding in this excellent...tool to try in your own work!) If you've got a research story you want to share on our blog, please reach...

...Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project Advice...shifts to choosing an unexplored and impactful research topic that aligns with your interests. We've outlined... Important factors to consider when choosing a research project After joining a lab, the next important...project will I work on?” In the short term, the research project must have innovative yet attainable milestones...the scientific community. In the long term, the research project establishes your professional brand. It...good chuck of your mental energy and time. The research project also serves as a stepping stone into the...questions can serve as a guide when selecting a research project. What will the impact be in a best case...

...viral vectors repository this year (and it's only March!)  In our repository, you'll find the latest iteration...

...registration please go to http://futureofresearch.org/boston/. The Future of Research Symposium A year ago, trainees...and sustainable biomedical research sector at our first Future of Research symposium. The talks and workshops...Shaping the Future of Research: a perspective from junior scientists." F1000Research 3 (2014). Pubmed PMID...The second Boston Symposium on the Future of Research will be held from 22-24 October. This blog has ...been contributed by guest blogger and Future of Research Symposium organizer, David T. Riglar PhD. Here... panel discussions to be held at the Future of Research Symposium Boston 2015 – Academic Data and the ...discovered through the four workshops at the Future of Research Symposium 2014, young scientists are acutely aware...

...problem endemic to biomedical research: pervasive bias that is easy for researchers to ignore or miss. The lack...Biomedical research has a long and well-documented history of racial bias, which still impacts and influences...influences current research. Understanding the depth and breadth of this bias, and then using that understanding...lines took them down unexpected paths. Though this research uses ancestry groups rather than self-reported...self-reported race, it highlights the myriad issues researchers need to reflect upon before they attempt to make ...ancestral groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell ...used to make this information accessible to the research community. Sean Misek and Aaron Fultineer in the...

...another university to perform research   Why should I try research? Personally, I have always been...undergraduates interested in doing research and attending research conferences. The Barry Goldwater scholarship...we are. Funded Research Experiences for Undergraduates (REUs) and other summer research opportunities ...was contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at UC Berkeley...Berkeley. Most of the scientists I’ve met began doing research in their undergraduate years. We each have a unique...taking the first step into the world of academic research can be quite daunting. During my first year of...of college, I knew I wanted to try research, but had no idea what to expect. I had a lot of help from ...

...will help you find more new CRISPR tools for your research! This time: Optogenetic repression in yeast Base...They generated these editors using the BE4max architecture. The new editors reported in this study have...

...Future of Research" conference. You can register for the conference here. The Future of Research conference...jobs in academia. Certainly funding for academic research and training is getting tighter and competition...resources to do enough. There are still university research departments that have no formal career resources...

... academic researchers themselves(44% of postdocs in the life sciences name faculty-research as their preferred...and the Early Career Researchers in Publishing panel, focused on showing researchers that they can take ... recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally participated...incredibly important to the stability of the biological research enterprise going forward, in this post I’ll point...from the symposium. The goal of the Future of Research Symposium On the FOR website, the FOR Boston 2015...determine what data is required for the academic research community to develop a plan to make postdoctoral...outcomes, and increase diversity in the academic research environment. The “how many?” question lies at ...

...Plasmid Kit Retroviral Barcoding Library (Kit # 1000000097) The Winslow lab barcoding library consists of...human immunodeficiency virus (HIV) genome. For research purposes, retroviral vectors have been developed...Retroviral vectors make up a large toolbox used by researchers for (among other things) gene delivery, generating...empty MuLV vector from the Weinberg lab enables researchers to express siRNAs of their choice in mammalian...Cell Virus, MSCV) each with a unique 6 nucleotide barcode. This collection can be used for in vivo phenotypic...

...in-class research experience on their resumes). CUREs are not without challenges. Real research is messy...Course-based undergraduate research experiences can make scientific research more inclusive. CBE Life Sciences...excitement and motivation of research to students; Using inquiry and research-based learning in a year-long...biochemistry laboratory : Part II – research-based laboratory – a semester-long research approach using malate dehydrogenase...one-on-one mentoring. His solution? Bringing his research into the classroom. I had the opportunity to talk...now commonly termed a Course-based Undergraduate Research Experience (CURE). Having incorporated a discovery-based...

... this vector which has helped launch countless research projects and which represents a lasting tribute...Hartmut Land had set up shop at the Imperial Cancer Research Fund (ICRF) laboratories at Lincoln’s Inn Fields...cooperation (Land et al., 1983).  We initiated research on expression cloning of novel oncogenes beyond...orders each), this vector has aided countless researchers - a fitting tribute to my late father. Many thanks...

...Efforts to Support Rare Disease Research: IFOPA Donating Plasmids to Researchers in Developing Countries with...hear about breakthroughs in diabetes and lymphoma research or the many different ways you can improve your... patients to important resources and promoting research for rare disease cures. Through their BeHEARD ...Addgene will be used to accelerate rare disease research and provide hope to patients suffering from these...Spinal muscular atrophy Dr. Elia Di Schiavi is a Research Scientist at the Institute of Biosciences and ... Addgene often to learn of future rare disease research funding opportunities. References 1. Jones, C...Resources on Addgene.org Find Plasmids for Disease Research ...

...for lab equipment that will help accelerate his research. Listen to learn more about Dr. Kwapata’s current...agriculture 5:47-9:35 : Kingdom's biotechnology research at Michigan State University 9:36-10:29 : Kingdom's...the difficulties surrouding and need for biotech research in Malawi 11:41-14:53 : Kingdom's current projects... How Seeding Labs is helping advance Kingdom's research 28:08-END : Kingdom's Thoughts on his time in ...about Addgene Plasmid Donations for Rare Disease Research Resources on Addgene.org Become a part of the...

... to the repository that will help epigenetic researchers custom design their own engineering plasmid tools...

...Center for Rett Syndrome research. With a shared passion for facilitating research and discovery, Addgene...The Rett Syndrome Research Trust (RSRT) has an ambitious goal of curing Rett Syndrome and Addgene is ...biology tools so that both veteran and new Rett researchers can find the reagents and resources they need...blog post describes the history of Rett Syndrome research, the role of the MECP2 gene, and highlights some...respectively. One of the main challenges facing Rett researchers is how to deliver the precise amount of protein...brain cell to restore physiological levels. Much research is now focused on therapeutic strategies that ...expertise to develop a viable therapeutic, the researchers developed adeno-associated viral (AAV) vectors...

...they research, how they conduct their research, and in some cases, if they conduct their research. Even... Joanne Kamens, Marcy Patrick, Aliyah Weinstein, Fabienne Furt, and Quintin Marcelino for their helpful... Marc Zimmer’s recent book, The State of Science: What the Future Holds and the Scientists Making It ...inherent brokenness of scientific publishing and research funding. I’ve been aware of these problems since...