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We narrowed to 19 results for: bryan roth

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Showing: 1 - 19 of 19 results

  1. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Chemogenetics Bryan Roth New serotype pAAV-CaMKIIa-hM4D(Gi)-mCherry AAV1 Chemogenetics Bryan Roth New serotype...Chemogenetics Bryan Roth New serotype pAAV-hSyn-hM4D(Gi)-mCherry AAV1 Chemogenetics Bryan Roth New serotype...pAAV-Ef1a-fDIO EYFP AAV5. AAV8, AAVrg Control Karl Deisseroth New serotype (multiple) rAAV-CAG::FLEX-...pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE...

  2. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...pAAV-hSyn-DIO-EGFP Bryan Roth 50459 AAV1 pAAV-hSyn-DIO-mCherry Bryan Roth 50457 AAV1 pAAV-hSyn-DIO-EGFP Bryan Roth...Depositor 44361 AAV1 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Bryan Roth   Recombinases Recombinase systems such as...-Ef1a-DIO EYFP Karl Deisseroth 11447 AAV5 pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2 pOTTC1032...Roth 114472 AAVrg pAAV-hSyn-mCherry Karl Deisseroth 83895 AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Gordon...

  3. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...selective and effective. A new ligand from the Bryan Roth lab and Takafumi Minamimoto’s lab known as deschloroclozapine...pathways   Acknowledgements We thank the Bryan Roth lab for feedback on this article and sharing ...sharing their expertise with us. References Urban DJ, Roth BL (2015) DREADDs (Designer Receptors Exclusively...

  4. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...unknown ligands and their GPCR binding partner, Bryan Roth’s lab created an open source resource called PRESTO-TANGO...entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled a collection of plasmids ...expression of a luciferase reporter in the nucleus. The Roth lab showed that this platform accurately identified...Exclusively Activated by Designer Drugs (DREADDs) (Roth, 2016). DREADDs were engineered from members of ... X-P, Lansu K, McCorvy JD, Giguère PM, Sciaky N, Roth BL (2015) PRESTO-Tango as an open-source resource...e11 . https://doi.org/10.1016/j.cell.2017.03.028 Roth BL (2016) DREADDs for Neuroscientists. Neuron 89...

  5. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...AAV1 Controls Bryan Roth New serotype pAAV-Ef1a-DIO mScarlet AAV5 Controls Karl Deisseroth New serotype...serotype pAAV-hSyn-EGFP AAV PHP.eB Controls Bryan Roth New serotype AiP13044 - pAAV-AiE0779m_3xC2-...Optogenetics Karl Deisseroth New serotype pAAV-hSyn-hChR2(H134R)-EYFP AAV2 Optogenetics Karl Deisseroth New serotype...

  6. Chemogenetics AAV Preps

    Type
    Collection
    ...none 5 Bryan Roth 50478 pAAV-GFAP-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion none 5 Bryan Roth...fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry hM4D(Gi)...fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Bryan Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid...Inhibition IRES mCitrine Cre-dependent 8, rg* Bryan Roth 50454 pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine...Activation HA fusion and IRES mCitrine Cre-dependent 8 Bryan Roth 50455 pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine...Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Bryan Roth 50458 pAAV-hSyn-DIO-rM3D(Gs)-mCherry rM3D(Gs)...Activation mCherry fusion Cre-dependent 8, rg* Bryan Roth 50474 pAAV-hSyn-hM3D(Gq)-mCherry hM3D(Gq) - Activation...

  7. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...GPCR activation leads to downstream effects.  Bryan Roth’s lab created a collection of plasmids called ...localization of the Rluc8 was more challenging. The Roth lab used structure-guided protein engineering and... (see figure). What can TRUPATH be used for? The Roth lab used the TRUPATH plasmids in a number of different...thanks to Jeff DiBerto, a graduate student in the Roth lab, for editing the blog post. References Olsen...Krumm BE, Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT (2020) TRUPATH, an open-source biosensor...

  8. Retrograde AAV viral preps

    Type
    Collection
    ... Syn EGFP Control Bryan Roth 50469 pAAV-CaMKIIa-EGFP CamKII EGFP Control Bryan Roth 114472 pAAV-hSyn-mCherry...DREADD Bryan Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent DREADD Bryan Roth 50474...Cre-dependent DREADD Bryan Roth 50475 pAAV-hSyn-hM4D(Gi)-mCherry Syn Inhibitor DREADD Bryan Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...pAAV-hSyn-DIO-mCherry Syn mCherry, Cre-dependent Control Bryan Roth 59462 pAAV-CAG-tdTomato CAG tdTomato Control ...pAAV-hSyn-DIO-EGFP Syn EGFP, Cre-dependent Control Bryan Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and...pAAV-hSyn-hM3D(Gq)-mCherry Syn Activator DREADD Bryan Roth 121539 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) Syn ...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine Syn Inhibitor DREADD Bryan Roth 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA...

  9. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ... entirely of viruses expressing DREADDs from Bryan Roth’s lab at the University of North Carolina, Chapel...mCherry When using this virus for the first time, the Roth Lab recommends verifying cre expression prior to... Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation of AgRP...

  10. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...muscarinic-derived DREADDs. A ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine...References Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship...Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth BL (2016) DREADDs for Neuroscientists. Neuron 89...

  11. Control AAV Preps

    Type
    Collection
    ....eB Bryan Roth 50469 pAAV-CaMKIIa-EGFP CaMKIIa EGFP Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Bryan Roth... Karl Deisseroth 50457 pAAV-hSyn-DIO-EGFP hSyn EGFP Cre dependent 1, 2, 5, 8, 9, rg* Bryan Roth 50459 ...hSyn mCherry Cre dependent 1, 2, 5, 8, 9, rg* Bryan Roth 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent...Constitutive 8 Karl Deisseroth 131001 pAAV-hSyn-mScarlet Syn mScarlet Constitutive 1, 8 Karl Deisseroth 135630 pAAV-S5E2...Karl Deisseroth 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP Cre dependent 1, 2, 5, 9, rg* Karl Deisseroth 28306...Karl Deisseroth 137133 pAAV-Ef1a-Con/Foff 2.0-mCherry EF1a mCherry Cre dependent 8 Karl Deisseroth 137137...8 Karl Deisseroth 137162 pAAV-Ef1a-Con/Foff 2.0-EYFP EF1a EYFP Cre dependent 8 Karl Deisseroth 98927 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40...

  15. Serotype Testing AAV

    Type
    Collection
    ...AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin...

  16. Brain Initiative Collection

    Type
    Collection
    ...vector for hSyn driven KOR DREADD expression. Bryan Roth 75033-AAV1 pAAV CD68-hM4D(Gi)-mCherry Expression...Expression of hM4D(Gi) using the CD68 promoter. Bryan Roth 75033-AAV9 pAAV CD68-hM4D(Gi)-mCherry Expression...Expression of hM4D(Gi) using the CD68 promoter. Bryan Roth 83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent...

  17. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...DREADD) plasmids on his own, UNC-Chapel Hill's Bryan Roth has now deposited many of these constructs with...G-protein coupled receptors have been engineered by the Roth lab to be activated by otherwise pharmacologically... For more information on DREADDs, please see the Roth lab's DREADD users blog. New CRISPR plasmids...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...assays to monitor activation of these receptors. Bryan Roth's lab has a newly available PRESTO-TANGO kit which...
Showing: 1 - 19 of 19 results