...Chemogenetics Bryan Roth New serotype pAAV-CaMKIIa-hM4D(Gi)-mCherry AAV1 Chemogenetics Bryan Roth New serotype...Chemogenetics Bryan Roth New serotype pAAV-hSyn-hM4D(Gi)-mCherry AAV1 Chemogenetics Bryan Roth New serotype...pAAV-Ef1a-fDIO EYFP AAV5. AAV8, AAVrg Control Karl Deisseroth New serotype (multiple) rAAV-CAG::FLEX-...pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE...

...pAAV-hSyn-DIO-EGFP Bryan Roth 50459 AAV1 pAAV-hSyn-DIO-mCherry Bryan Roth 50457 AAV1 pAAV-hSyn-DIO-EGFP Bryan Roth...Depositor 44361 AAV1 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Bryan Roth   Recombinases Recombinase systems such as...-Ef1a-DIO EYFP Karl Deisseroth 11447 AAV5 pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2 pOTTC1032...Roth 114472 AAVrg pAAV-hSyn-mCherry Karl Deisseroth 83895 AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Gordon...

...selective and effective. A new ligand from the Bryan Roth lab and Takafumi Minamimoto’s lab known as deschloroclozapine...pathways   Acknowledgements We thank the Bryan Roth lab for feedback on this article and sharing ...sharing their expertise with us. References Urban DJ, Roth BL (2015) DREADDs (Designer Receptors Exclusively...

...unknown ligands and their GPCR binding partner, Bryan Roth’s lab created an open source resource called PRESTO-TANGO...entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled a collection of plasmids ...expression of a luciferase reporter in the nucleus. The Roth lab showed that this platform accurately identified...Exclusively Activated by Designer Drugs (DREADDs) (Roth, 2016). DREADDs were engineered from members of ... X-P, Lansu K, McCorvy JD, Giguère PM, Sciaky N, Roth BL (2015) PRESTO-Tango as an open-source resource...e11 . https://doi.org/10.1016/j.cell.2017.03.028 Roth BL (2016) DREADDs for Neuroscientists. Neuron 89...

...AAV1 Controls Bryan Roth New serotype pAAV-Ef1a-DIO mScarlet AAV5 Controls Karl Deisseroth New serotype...serotype pAAV-hSyn-EGFP AAV PHP.eB Controls Bryan Roth New serotype AiP13044 - pAAV-AiE0779m_3xC2-...Optogenetics Karl Deisseroth New serotype pAAV-hSyn-hChR2(H134R)-EYFP AAV2 Optogenetics Karl Deisseroth New serotype...

...GPCR activation leads to downstream effects.  Bryan Roth’s lab created a collection of plasmids called ...localization of the Rluc8 was more challenging. The Roth lab used structure-guided protein engineering and... (see figure). What can TRUPATH be used for? The Roth lab used the TRUPATH plasmids in a number of different...thanks to Jeff DiBerto, a graduate student in the Roth lab, for editing the blog post. References Olsen...Krumm BE, Slocum ST, Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT (2020) TRUPATH, an open-source biosensor...

... entirely of viruses expressing DREADDs from Bryan Roth’s lab at the University of North Carolina, Chapel...mCherry When using this virus for the first time, the Roth Lab recommends verifying cre expression prior to... Rogan SC, Adams AC, Cusher DS, Maratos-Flier E, Roth BL, Lowell BB. Rapid, reversible activation of AgRP...

...muscarinic-derived DREADDs. A ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine...References Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015) The First Structure–Activity Relationship...Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth BL (2016) DREADDs for Neuroscientists. Neuron 89...

...People get hooked on sharing because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing ...

... tools. A variety of chemogenetic tools from Bryan Roth’s lab are already available allowing you to chemically...

...high-quality protocols for lentiviral production. Bryan Roth’s lab from the University of North Carolina, Connie...

...AAV are made from plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin...

...vector for hSyn driven KOR DREADD expression. Bryan Roth 75033-AAV1 pAAV CD68-hM4D(Gi)-mCherry Expression...Expression of hM4D(Gi) using the CD68 promoter. Bryan Roth 75033-AAV9 pAAV CD68-hM4D(Gi)-mCherry Expression...Expression of hM4D(Gi) using the CD68 promoter. Bryan Roth 83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent...

...DREADD) plasmids on his own, UNC-Chapel Hill's Bryan Roth has now deposited many of these constructs with...G-protein coupled receptors have been engineered by the Roth lab to be activated by otherwise pharmacologically... For more information on DREADDs, please see the Roth lab's DREADD users blog. New CRISPR plasmids...

...assays to monitor activation of these receptors. Bryan Roth's lab has a newly available PRESTO-TANGO kit which...

...congrats for 1 million orders. @RothLabUNC accounts for 10,000 or 1%! — BryanRoth (@zenbrainest) August 21, ...