Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
Type
Blog Post
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How do Cas13d enzymes compare to other Cas13s?
Like other Cas13 enzymes, the Cas13d orthologs described...cleavage does not occur when Cas13d is expressed in a mammalian system.
Since Cas13d is functionally similar...
One interesting twist to Cas13d enzymes is their cleavage pattern: EsCas13d produced very similar cleavage...from Konermann et al. and Yan et al. describes new Cas13d enzymes that average only 2.8 kb in size and are...the transcriptome engineering toolbox.
How were Cas13d enzymes discovered?
Microbial CRISPR diversity ...the wealth of CRISPR possibilities. To identify Cas13d, both groups used very general bioinformatic screens...repeat array near a putative effector nuclease. The Cas13d proteins they identified have little sequence similarity...