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We narrowed to 14 results for: cd4

Showing: 1 - 14 of 14 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...out all CD8+ T cells from a mix of CD8+ T cells, CD4+ T cells, B cells, and dendritic cells. In this case...except CD8+ T cells. We might choose the markers CD4, CD19, and CD11c, which would label everything but...excluded, or “dumped”. From left to right: B cell, CD4+ T cell, dendritic cell, CD8+ T cell. Created using...

  2. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...HIV-1 infects cells in the immune system, notably CD4+ T-cells, and eventually leads to acquired immunodeficiency...T-cells to see if they could be rescued by CRISPR. CD4+ T-cells were isolated from healthy individuals, ...reduced the HIV-1 copy number by over 50% in isolated CD4+ T-cells from two HIV-1 infected patients. Although...

  3. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...focus on characterizing those epitopes as CD8 or CD4 epitopes, as well as their potential binding affinity... or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can be further...

  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... substitute for HIV-1 Env, which can only infect CD4+ cells. 3rd generation lentiviral packaging system...the first clinical trial with a lentiviral vector. CD4+ T-cells from patients with HIV-1 were transduced...

  7. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Murine Leukemia Virus (MuLV) and HIV glycoprotein — CD4+ cell transduction Pseudotyped retroviral vectors...retroviral vector with specificity of infection for CD4-expressing cells. Proceedings of the National Academy...

  9. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...effector immune cells. TGFβ is also known to change CD4+ Th cells into regulatory cells to suppress function...metastasis. Evading Recognition Tumor cells over-express CD47 to escape recognition by circulating macrophages...

  10. Immunology Research Plasmids and Resources

    Type
    Collection
    ...FLJ94613, MGC138597, T3G CD4 CD4 molecule CD4mut CD40LG CD40 ligand CD154, CD40L, HIGM1, IGM, IMD3, T-BAM..., CLEC4L, DC-SIGN, DC-SIGN1, MGC129965 CD4 CD4 molecule CD4mut CD74 CD74 molecule, major histocompatibility...TNFRSF5, p50 CD40LG CD40 ligand CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L CD70 CD70...CD3Z, T3Z, TCRZ CD48 CD48 molecule BCM1, BLAST, BLAST1, MEM-102, SLAMF2, hCD48, mCD48 CHP calcium binding...MGC116710, MGC34104 CD320 CD320 molecule 8D6, 8D6A CD40 CD40 molecule, TNF receptor superfamily member 5 Bp50...tyrosine phosphatase, receptor type, C B220, CD45, CD45R, GP180, LCA, LY5, T200 RASGRP1 RAS guanyl releasing...PROCR protein C receptor, endothelial (EPCR) CCCA, CCD41, CD201, EPCR, MGC23024, bA42O4.2 PSMB8 proteasome...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...bio-His ADAM10 His, CD4 CMV Alzheimer's Gavin Wright 51643 APP-bio-His APP His, CD4 CMV Alzheimer's Gavin...Gavin Wright 51811 PRNP-bio-His PRNP His, CD4 CMV Dementia Gavin Wright 53363 pCB268 APP Flag, HA, Myc ...

  12. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...protein for the HEK 293T cells to express: CD45. Upon expression, CD45 is incorporated into the plasma membrane... manage to express CD45. Thus, if transduction efficiency is much higher than CD45 expression, this could...our flow experiment, we'll want to identify CD45+ cells (CD45 expression) and assess our transduction efficiency...our protein of interest: CD45. I am using a conjugated antibody to detect CD45, and I'll need to choose...transduction reporter. Note that eGFP is not fused to CD45, but is separately expressed. (The reason for having... fluorophore my antibody is conjugated to. Since CD45 is a common marker, finding an antibody already ...transgenic protein. We don't know yet how abundantly CD45 will be expressed on the transduced cells, so it's...
Showing: 1 - 14 of 14 results