...particular vector by excising the insert from the backbone. To do this, you'll use enzymes with restriction...to know both the approximate size of the vector backbone as well as the predicted size of the insert. You...bands at 6kb and 1.2kb (red box), matching the backbone and insert, respectively. The results on the gel...

... NGS result to a reference sequence to confirm backbone elements.   Confirming the gene/insert by aligning...NGS result to a reference sequence like a known backbone sequence. We’re not necessarily expecting a perfect...mismatches in the origin of replication or other common backbone elements. Since we’ve successfully grown the plasmid...

...vector backbone from the Shih lab. You can find more lentiviral expression vectors (empty backbones) here...Plasmid #52961) This is a 3rd generation lentiviral backbone from the Zhang lab expressing S. pyogenes Cas9...

...species. These plasmids all have a PR-T4 vector backbone, which is a modified version of L440. This vector...cloning to insert the cDNA into the desired plasmid backbone. The Sánchez lab plasmids allow students and new... gene of interest into the empty PR-T4P vector backbone. The empty vector PLL4, which is similar to PR-T4P...

... of the essence. This method requires a vector backbone containing a topoisomerase I cut site, toposisomerase...the topoisomerase I generated overhangs in the backbone. Once combined, the cut and ligation processes...out protocols for plasmid cloning Find vector backbones Read our molecular biology reference ...

...peptides. PX459 This is the vector backbone. This particular backbone can be used to transfect a variety...

...2006, we saw an increase in popularity of empty backbones for shRNA expression, such as pLKO.1 puro and ...shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral expression and...

...links can help you calculate how much insert and backbone you need for different types of cloning methods...method. You can select from five different insert:backbone ratios. In-Fusion cloning molar ratio calculators...

...parental antibody (blue, rat) are cloned into a backbone expressing the constant regions from an alternative... a specific antibody are cloned into a plasmid backbone containing all constant regions, just the heavy...

...Check Out Addgene's Empty Backbone Resource: Choosing Your Perfect Plasmid Backbone Read More: Plasmids...

... SARS-CoV-2 expression plasmids in a high copy backbone with a T7 promoter driving multiple combinations...plasmids. Each combination is present in two sets of backbones which drive expression using either TEF1 or GAL1...

...blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies on this plate...list of Bacterial Expression Systems Find Empty Backbones for Your Experiments    ...

...create an amplicon that  was cloned into a plasmid backbone for expression of the mAb heavy and light chain...modify the CH encoding sequence within the plasmid backbone to generate R-mAbs of different subclasses, including...

...while expressing the gene. Briefly, the vector “backbone” in this system contains ccdB. A successful insertion...propagate and prep your ccdB-containing empty backbones but should never be used when selecting for recombinant...

...that target the common features of viral vector backbones; once validated these universal primers can be... for functional assays depending on the vector backbone. When using qPCR based methods for viral titration...

...the desired mutation and excision of the vector backbone. To do this, rhamnose is added which induces expression...point will be those that have excised the vector backbone. Vectors pTOX4, pTOX5, and pTOX6 express the ...

...plasmid backbone that was designed specifically for transient expression (find some useful backbones here...

...protocol!  First, the authors created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector commonly...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

...track: Compatibility: Double-check your plasmid backbone and expression strain to make sure they are compatible...below or browse Addgene’s collection of empty backbones.   Our Most Popular Plasmids 101 Posts: Origin...

...interest (Figure 2). 1. The phosphoserine OTS The backbone of the phosphoserine OTS, Sep-OTS, contains the...using the PhosTag system (see below). This plasmid backbone can also be cloned with an ORF of interest for...