Addgene

We narrowed to 200 results for: ckb

Showing: 61 - 80 of 200 results

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A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

Type

Blog Post

...pBLADE-mCherry: This construct, built from the pBAD33 backbone, contains BLADE, the PBAD promoter, and a mCherry...
In Vivo Biotinylation of Bacterial Fusion Proteins

Type

Blog Post

...create a plasmid based on the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful...
Viral Vectors 101: Inverted Terminal Repeats

Type

Blog Post

...immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these sequences...
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

Type

Blog Post

...Cloning Technique Choosing Your Perfect Plasmid Backbone ...
FlipGFP, a novel fluorescence protease reporter to study apoptosis

Type

Blog Post

...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...
Hot Plasmids - January 2023

Type

Blog Post

...and RNA-seq of a nuclear (MALAT1) and cytoplasmic (CKB1) RNA target. Adapted from Figure 3 Colognori et ...
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

Type

Blog Post

...domains The original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment (PDGFR ...
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

Type

Blog Post

...CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12 (Figure 2). Cloning from the pAWp28 storage...
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

Type

Blog Post

...Biology Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ...
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Type

Blog Post

...lentivirus. Also, note that due to the size of the backbone, transformations require electroporation. References...
AAV Q&A with Tim Miles

Type

Blog Post

... engineered AAV are presently built on an AAV9 backbone and so will very likely cross-react with each ...
15 Years of Addgene: The Top 15 Plasmids

Type

Blog Post

...-1a promoter. It’s a 3rd generation lentiviral backbone that must be used with lentiCas9-Blast or with...
A Practical Approach to Choosing the B(right)est Fluorescent Protein

Type

Blog Post

...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...
Luminescent Imaging with Nano-lanterns

Type

Blog Post

...Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein Tags ...
CRISPRainbow and Genome Visualization

Type

Blog Post

...Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ...
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

Type

Blog Post

...disrupted interactions between Cas9 and the phophate backbone of the target DNA strand via mutations N497A, ...
Developing Lab Management Software for Biology

Type

Blog Post

...plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you simply don't have enough context...
Troubleshooting Your Plasmid Cloning Experiment

Type

Blog Post

...try to use an excess of insert compared to the backbone plasmid; a starting point could be a molar ratio...
Plasmid Tools for Microbiome Studies

Type

Blog Post

...toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin...
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Type

Blog Post

...promoters were also tested in different plasmid backbones that varied in size or carried different genes...

Showing: 61 - 80 of 200 results

Results per page:

A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

In Vivo Biotinylation of Bacterial Fusion Proteins

Viral Vectors 101: Inverted Terminal Repeats

Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

FlipGFP, a novel fluorescence protease reporter to study apoptosis

Hot Plasmids - January 2023

New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

AAV Q&A with Tim Miles

15 Years of Addgene: The Top 15 Plasmids

A Practical Approach to Choosing the B(right)est Fluorescent Protein

Luminescent Imaging with Nano-lanterns

CRISPRainbow and Genome Visualization

Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

Developing Lab Management Software for Biology

Troubleshooting Your Plasmid Cloning Experiment

Plasmid Tools for Microbiome Studies

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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