...oligos are required and the BsaI linearized pEcgRNA backbone can be prepared in large amounts and frozen for...

...means that the promoter present in your plasmid backbone must to be compatible with the type of RNA that...

...create the pCell-Free vectors, Gateway-compatible backbones that enable cell-free expression of proteins in...

...the Fluorescent Protein Collection Find empty backbones for fluorescent protein fusions Browse the Fluorescent...

...the desired mutation and excision of the vector backbone. This step requires “counter-selection,” meaning...

...interest, painstakingly cloned into the perfect empty backbone, made sure to add the right tags to your gene,...

...the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse the fluorescent...

... (single copy, bacterial artificial chromosome backbone) contains the register. Depending on the desired...

...still think “but that won’t happen to me, I have a backbone”. The sad truth is, if it happened before, it ...

...construct, where your RNAi-target is fused to the DNA backbone that contains a target sequence against the fluorescent...

...Protein Resources Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ...

...Addgene.org Find Photoactivatable FPs in Empty Backbones in the Michael Davidson Collection Find Photoactivatable...

... pMAZ-SK contains an aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through...

... pMAZ-SK contains an aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through...

...Often, the size of the plasmid insert and vector backbone are known and thus this technique can be quickly...buffer Verifying Total Plasmid Size -OR- Insert and Backbone Size The simplest form of diagnostic digest is...the expected size, or that it is composed of a backbone and insert of expected sizes. This is frequently...in two bands; the 1200bp insert and the 5000bp backbone. Verifying Plasmid Identity by "Plasmid Fingerprinting...know that you have a 1,200bp insert in a 5,000bp backbone, but there are many plasmids out there that, when...very unique pattern that will distinguish one 5kb backbone with a 1.2kb insert from all others. When choosing...orientation the insert is ligated into the vector backbone and therefore the only thing left to do is identify...

...components are provided in Golden Gate compatible backbones that can be combined with your gRNA and repair...

...Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 ...Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs ...library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs...genes and containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting...VP64) Set A Human CRISPR inhibition library in backbone pXPR_050 inhibiting 18,901 genes and containing...

...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains...resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...LacZ, plus Cre recombinase 60229 : sgRNA cloning backbone with Cre recombinase 60230 : sgRNA targeted to...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described ...control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid ...

...digested vector backbone. This is accomplished by covalently connecting the sugar backbone of the two DNA..., the insert DNA is physically attached to the backbone and the complete plasmid can be transformed into..., it is important to treat the digested vector backbone with a phosphatase before performing the ligation...

...plasmids for a given species are based on the same backbone to permit expression levels to be directly compared...