...8395 p210 pCMV-CREM CREM CMV Mammalian Green 8401 p224 pCMV-RFP/CREM floxed RFP within CREM CMV Mammalian...Green 8404 p236 pPr-CREM CREM probasin Mammalian Green 8405 p240 pPr-CREM/Ins CREM with insulators probasin... pPr-CREM/CMV-STOP-luc CREM and CMV-STOP-luc cassette probasin Mammalian Green 8408 p255 pPr-CREM/Ins/...p259 pCMV-CRE-M (BglII) CREM CMV Mammalian Green 12494 p260 pCMV-CRE-M-AR(wt) CREM fused to AR ligand binding...facilitating high rates of Cre-driven recombination. CREM (or Cre-M) has been engineered to contain an intron...Ins/CMV-STOP-luc CREM and CMV-STOP-luc cassette, separated by insulator probasin Mammalian Green 11543 pPGK-Cre-bpA... Mammalian Green 12496 p270 pCMV-CRE-M-AR(LNCaP) CREM fused to mutant AR ligand binding domain CMV Mammalian...

...without exclusivity. Chapter 11: The Stem Cell Bank (CReM Bank) at the Center for Regenerative Medicine and...

...or polypeptides to a solid substrate (Holden and Cremer, 2003). References 1. Chapman-Smith, A., & Cronan...588–600. PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled...

...is to do it huge, there are times when a more incremental approach is prudent. What's in the CRISPR kinome...

...develop dCas9 for synthetic looping, the Phillips-Cremins lab developed the light-activated-dynamic-looping...

... any other experimental approach, use small, incremental changes to understand what works and what doesn't...

...wavelength as it has been shown that even a small incremental change of the excitation wavelength can significantly...

...Gilgenast TG, Gong W, Beagan JA, Raj A, Phillips-Cremins JE (2019) LADL: light-activated dynamic looping...

...puromycin should be from 1-10 μg/mL in 1 μg/mL increments. d. Label plates from 1-10 and add appropriate... may wish to repeat this titration with finer increments of puromycin to determine a more precise optimal...

...Immediately purify them! Commercial Maxipreps rely on incremental, ordered cell lysis. E. coli cells are subject...

...CRLF2Y, TSLPR CRLF3 cytokine receptor-like factor 3 CREME9, CYTOR4, FRWS, MGC20661 CSF1 colony stimulating...