Plasmids 101: Screens vs. Selections
Type
Blog Post
... Addgene Blog
CRISPR libraries
Genome-wide screens with CRISPR
Lentiviral CRISPR libraries
...with small molecules/drugs.
With the advent of CRISPR, genome-wide knockout screens are possible with...While the entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically...downstream manipulations as a forward or reverse screen (CRISPR, drug, etc.), just with an added baseline component...component.
Alternative screening techniques
While CRISPR and drugs dominate the screening scene, there are... worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes of interest... be lethal to cells and thus not assayable with CRISPR. Another method, popularized in yeast, are temperature...