Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
Type
Blog Post
...the Addgene blog
CRISPR101: Editing the Epigenome
Mapping the 4D Nucleome with CRISPR/Cas9
Resources ...introduce you to a simpler, yet more effective, CRISPR-based epigenetic approach (spoiler alert: it’s ...dioxygenase
When I started to work on building a CRISPR/dCas9-based tool for DNA demethylation in 2015,...al. 2018, Kaas et al. 2013).
Figure 2: CRISPR TET-based epigenetic editing. In reality, editing...Moshe Szyf at McGill University, working to develop CRISPR-based tools for epigenetic editing.
References...Resources at Addgene.org
Read our CRISPR Guide to Epigenetics
References
Charlton J, Jung EJ, Mattei AL et al...targeted steric blockage of DNA methyltransferase with CRISPR/dCas9. Nat Commun 12, 5711. https://doi.org/10.1038...