...target CpG, it physically blocks DNMT1 from that site. So, when DNMT1 methylates the genome during DNA...Dividing cells depend on DNA methyltransferase 1 (DNMT1) to copy methylation marks from parental to daughter... Figure 3: A simpler approach - dCas9 blocks DNMT1 methylation, leaving the target CpG unmethylated...inside the gRNA sequence is a great guarantee that DNMT1 will be blocked (within PAM works, too), but if ... binding is crucial for dCas9 to interfere with DNMT1 even through replication. The dCas9 also needs to...

...pSico Dnmt1 DNMT1 GFP CMV Hereditary sensory neuropathy type IE Tyler Jacks 12166 pSicoR Dnmt1 DNMT1 GFP... 16648 DNMT1 KO Hyg DNMT1 T7 Hereditary sensory neuropathy type IE Bert Vogelstein 16649 DNMT1 KO Neo ... pSMP-DNMT1_1 DNMT1 Hereditary sensory neuropathy type IE George Daley 36383 pSMP-DNMT1_2 DNMT1 Hereditary...His, GFP, HA CMV ALS Nico Dantuma 24952 LZRS-DNMT1 DNMT1 Hereditary sensory neuropathy type IE Paul Khavari...V5 EF-1 alpha Parkinson's Mark Cookson 25165 DNMT1 DNMT1 His T7 Hereditary sensory neuropathy type IE ... telangiectasia Keith Joung 36939 pcDNA3/Myc-DNMT1 DNMT1 Myc CMV Hereditary sensory neuropathy type IE...Parkinson's Niels Gehring 66818 pCCL-PGK-SPdCas9-BFP-DNMT1 DNMT1 PGK Hereditary sensory neuropathy type IE Yonglun...