...target CpG, it physically blocks DNMT1 from that site. So, when DNMT1 methylates the genome during DNA...Dividing cells depend on DNA methyltransferase 1 (DNMT1) to copy methylation marks from parental to daughter... Figure 3: A simpler approach - dCas9 blocks DNMT1 methylation, leaving the target CpG unmethylated...inside the gRNA sequence is a great guarantee that DNMT1 will be blocked (within PAM works, too), but if ... binding is crucial for dCas9 to interfere with DNMT1 even through replication. The dCas9 also needs to...