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We narrowed to 10 results for: fire lab vector kit

Showing: 1 - 10 of 10 results
  1. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

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    ...requested! Fire Lab C. Elegans Kit - Our first popular "kit," Andrew Fire's set of vectors for C. elegans...popular kit of the year was the Golden Gate TALEN Kit, which continues to be our most requested kit of the...expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids from Didier Trono's lab are our...cells. Plasmids from Shinya Yamanaka's lab and James Thomson's lab were the most frequently requested. Ubiquitin...first distributed plasmid and was created in the lab of my thesis advisor, Dr. Bruce Spiegelman. It was...expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar with...technology has been used and modified by so many labs and for so many different purposes that we had to...
  2. Worm Expression Resources

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    ...website. General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research...and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with and without...Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate...regarding this kit can be found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen...the collection. Return to top Plasmid Kits and Collections Kits are sets of plasmids intended for use ...found below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein Lab. Contains a set of plasmids...Jorgensen Lab. A collection of plasmids that streamlines the production of targeting vectors for tag insertion...
  3. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    Blog Post
    ...selection marker (Kim et al., 2014). Meanwhile Andy Fire’s lab used oligo-mediated knock-in of a dominant mutation...SapTrap CRISPR/Cas Toolkit and SapTrap-SEC kit for C. elegans genome engineering have also facilitated CRISPR... C. elegans. These modular toolkits build single plasmid targeting vectors that encode the gRNA transcript...The first approach – co-CRISPR from Craig Mello’s lab – used inactivation of the unc-22 gene as their selection...past few years:  Repair template design The Meyer lab meticulously characterized several aspects of editing...repair templates (Farboud et al., 2019).   The Mello lab also reported that modifying the 5’ modification ...2020). Together, these improvements from the Mello lab resulted in strikingly high editing efficiencies,...
  4. 22 Hot Plasmid Technologies from 2014

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    ...mini-transposon vector for bacterial mutagenesis or gene targeting Victor de Lorenzo's lab has engineered... existing transposon and vector systems and creating an all-synthetic vector that included only the elements...elements needed for function. The lab validated this vector, called pBAM1, by conducting random mutagenesis...”) by Margaret Robinson’s lab, and more recently used by Stephen Royle’s lab, acutely depleted proteins... into a destination vector in a predefined order. Finally, the destination vectors are used with Multisite... eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum...RNAi transgenic mice The Jackson-Grusby lab has designed two vectors for use in conditional and reversible...
  5. 27 Hot Plasmids from 2016

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    ... New kit for editing dicotyledonous plants The Stuttmann lab generated the vectors in this kit to make...Design Automation Research lab Moduclar Cloning (CIDAR MoClo) Parts Kit. This Kit consists of a variety of...plasmids in this kit are derived from the Ralser lab's own pHLUM minichromosomal vector, and besides being...these receptors. Bryan Roth's lab has a newly available PRESTO-TANGO kit which allows researchers to interrogate...Examples of FusX-Compatible Destination Vectors Available at Addgene: Lab Plasmids Dan Carlson RCIscript-GoldyTALEN...both laboratory and prototrophic strains. The vectors were based on the original EasyClone vectors (Jensen...expression vectors with various promoters suitable for in vivo expression, and/or produce vectors to make...
  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab Worm unc-...promoters C. elegans vector kit - Collection of plasmids from the Fire Lab L4440 - RNAi in ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...engineering Luciferase Reporter pGL3 Basic Vector - Firefly luciferase vector for investigating regions controlling...expression vector with N-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF ...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc...
  7. 28 Hot Plasmid Technologies from 2015

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    .... The MXS-chaining kit contains 5 empty chaining vectors plus a destination vector and a collection of... time. A Ramm lab plasmid with an N1-vector-compatible LOC8R sequence is also available, N1-LOC8R.  ...Cohen lab wished to improve on the Arch indicator and their collaborators in Robert Campbell’s lab carried...targeted fluorescent molecules. The Vale lab and the Weissman lab have teamed up and advanced technology...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...
  8. Luciferase Plasmid Collection

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    ...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...control of neuronal signaling. TRUPATH Kit , ONE-GO Biosensors Kit , and G-CASE : BRET-based sensors for...pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream of IRES-firefly luciferase...renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...Postovit 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic...Daniel Christophe 119760 pSGDlucV3.0 Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer...Kevin Janes 99283 pCW57.1-Luciferase Firefly TRE Lentiviral vector with dox- or tet-inducible luciferase...
  9. Bacterial Expression Systems

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    ... cpFRET - FRET-based Biosensors Kit (Addgene #1000000021) is a vector library for the generation of FRET...The MoBiFc Toolkit (Addgene #1000000222) is a collection of Golden Gate compatible vectors for bimolecular...selection of mammalian and bacterial FRET-related vectors and standards. The Hoschschild Bacterial Two-Hybrid...plants. The Zinc Finger Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain plasmids...Visit our TALEN Collection page to find plasmids and kits for efficient assembly of TALEN constructs. The ...England Biolabs Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and visualization...subtilis , and Pichia pastoris . The EcoFlex MoClo Toolkit (Addgene #1000000080) features a library of promoters...
  10. Fluorescent Protein Guide: Biosensors

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    ... cerevisiae cpFRET Kit includes plasmids to construct custom biosensors ScEnSor Kit includes fluorescent...) Li Lab GRAB_Ado Plasmids Yulong Li ADP Biosensor for ADP based on tetramethylrhodamine-labeled ParM ... Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells...based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (... calcium ion indicators FR-GECO1 Robert Campbell Lab (unpublished) Robert Campbell Calcium Far-red genetically...AMP) Green cAMP biosensors FLAMP1 and FLAMP2 Chu Lab FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based...
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