Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 4 results for: g deleted rabies virus production

Showing: 1 - 4 of 4 results

  1. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...with a deletion-mutant rabies virus. Retrograde neuronal tracing with a deletion-mutant rabies virus. Pubmed... protein called rabies glycoprotein (G). G-protein-coated RABV infects cells via the G receptor. In neurons...supplying the G protein in trans only to starter cells (infected with G-deleted RABV). In this case, G-deleted...mediated by the G protein/G-protein receptor. Once in first order neurons, the G-deleted RABV can divide...Spread and pathogenic characteristics of a G-deficient rabies virus recombinant: an in vitro and in vivo study...RABV applications Use of G deleted RABV in conjunction with restricted TVA and G protein expression is a...neuroscience. In this post, I’ll describe how rabies virus (RABV) can be used in the brain to visualize...

  2. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...cell body. Rabies virus and the EnvA-TVA system Another way to use rabies virus is to delete the glycoprotein...because of rabies virus’s toxicity, it made more sense to pseudotype another virus with rabies glycoprotein...only done on enveloped viruses such as lentivirus, retrovirus, and rabies virus. How to pseudotype To ...in. For example, production of vesicular stomatitis virus, HIV-2, and human foamy virus in cell lines expressing... the rabies glycoprotein. The glycoprotein will be incorporated into the replicating rabies virus envelope...) Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods 4:47–49 . https://doi.org...vesicular stomatitis virus G protein. VSV-G is a trimeric protein that binds phosphatidylserine and low-density...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...up to 120 kb) deletions in N. benthamiana and Arabidopsis. The authors found that deletion efficiency was...Saccharomyces cerevisiae, as a key host for the production of their products, including fermented beer and wine, ...respectively) and 2) the size of the deletion. Smaller deletions generally occurred at higher frequency...library to screen for genes involved in West Nile Virus (WNV) induced cell death. The Wu lab library consists...ingredients, and recombinant proteins. In order to keep production yields high and costs low, and to deal with the.... PubMed PMID: 26837755   SEPTEMBER 2016 Zika virus plasmids Now avaliable through Addgene The Vaithi... Addgene’s repository. The genes, from the Zika virus Asian genotype PRVABC59, were obtained from the ...

  4. Lentiviral Vector Guide

    Type
    Guide
    ...gene env (usually VSV-G due to wide infectivity) VSV-G (vesicular stomatitis virus G protein) is the most...gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein with broad tropism...types of lentivirus include Human immunodeficiency virus (HIV), bovine immunodeficiency virus (BIV), and...also contain a deletion in the 3’ LTR to make them self-inactivating (SIN). This deletion is transferred... a host cell, this deletion prevents further transcription of the full-length virus. The potential for...cell’s genome. Pseudotyping The production of viral vectors (or viruses) using viral envelope proteins ... that a virus can infect and replicate in. Viral vector The modified form of a wild-type virus used to...
Showing: 1 - 4 of 4 results