Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 21 - 40 of 57 results

  2. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...Institute for Biophysical Chemistry, Göttingen in Germany. I attended this symposium seeking inspiration ...Institute for Brain Research in Frankfurt am Main, Germany. She presented a novel technology, back then still...

  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed over 65 targets...., Croteau, J., Davis, J., Hernandez, J. M., & Germain, R. N. (2020). IBEX: A versatile multiplex optical...

  4. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ..., including 129 and C57BL/6, for which we have germline competent ESC lines. Second, the process is much...However, with only 3.4% of founders establishing germline transmission of the transgene, this technique ...

  5. Boxem Lab CRISPR Plasmids

    Type
    Collection
    ...16.48 heat shock promoter. All vectors include the germline expression compatible tbb-2 3’UTR. For both the...

  6. FlyCRISPR

    Type
    Collection
    ...generated in Drosophila and transmitted through the germline. Using an injection approach, stable lines with...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...RT-PCR. J. Immunol. Methods 283:291–306.Panina, Y., Germond, A., Masui, S. et al. Validation of Common Housekeeping...00292rv02. PMID: 10948434.Taylor SC; Laperriere G;  Germain H. Droplet Digital PCR versus qPCR for gene expression...

  8. Worm Expression Resources

    Type
    Collection
    ..., which diminishes epigenetic silencing in the germline. A "FLP-Out" system for controlled gene expression...

  9. p53 Pathway

    Type
    Collection
    ...2009 Oct;9(10):724-37. PubMed PMID: 19776742 . Germline TP53 mutations and Li-Fraumeni syndrome. Varley...

  10. TALEN Plasmids and Kits

    Type
    Collection
    ... is suitable for the TALEN-based generation of germline mutations in Bombyx mori and Drosophila melanogaster...

  17. RUBY-Red Siliques

    Type
    Blog Post
    ...untransformed seed from the same plant beginning to germinate. (Top right, second panel) Transformed seedlings...
Showing: 21 - 40 of 57 results