...12247 pLVTHM Expresses shRNA under the H1 promoter; shRNA or a H1-shRNA cassette from another vector (e.g...generation; Transgene (CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer...generation; Transgene (CAG promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick ...generation; Transgene (hEF-1alpha promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer...generation; Transgene (hPGK promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer...generation; Transgene (hPGK promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) Off Patrick ...generation; Transgene (hUbiquitin promoter) ‐ OR ‐ shRNA (H1 promoter when subcloned from pLVTHM) On Patrick Aebischer...

...Plant 8(8) (2015): 1274-1284. PubMed PMID: 25917172. Find plasmids from this paper at Addgene. 8. Xie...downstream of the 7SK, human U6, mouse U6, or human H1 promoters. If you express fewer than four gRNAs, ...Plasmids: These plasmids can be used to express up to 8 gRNAs after Golden Gate or Gibson Assembly. Using ...containing vector using Golden Gate assembly, and up to 8 gRNAs may be expressed simultaneously. Vectors for...

...your target gene! Tanenbaum et al., Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. Multi-bit genetic memory...expression levels. Zhang et al., PLoS One. 2012;7(8):e43072. doi: 10.1371/journal.pone.0043072. Photoswitchable... (U6) expression, shRNA/shRNA-miR30 constitutive (H1, U6, 7SK) or inducible shRNA-miR30 (CMV-TO) expression...Boncompain et al., Nat Methods 2012. Mar 11;9(5):493-8. MXS cloning platform for imaging and flow cytometry...blocks including 14 different fluorescent proteins, 8 constitutive promoters, 2 includible promoters, 3 ...Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. Plasmid tools for all-optical electrophysiology ...

...chemokine (C-C motif) ligand 23 CK-BETA-8, CKb8, Ckb-8, Ckb-8-1, MIP-3, MIP3, MPIF-1, SCYA23 CCL24 chemokine...heavy diversity 2-21 IGHD221 IGHD2-8 immunoglobulin heavy diversity 2-8 DLR1, IGHD28 IGHD3-10 immunoglobulin...69 IGHV1-E, IGHV169, IGHV1E IGHV1-8 immunoglobulin heavy variable 1-8 IGHV18 IGHV1-C immunoglobulin heavy...kappa variable 1-6 IGKV16, L11 IGKV1-8 immunoglobulin kappa variable 1-8 IGKV18, L9 IGKV1-9 immunoglobulin...1D-43 IGKV1D43, L23, L23a IGKV1D-8 immunoglobulin kappa variable 1D-8 IGKV1D8, L24, L24a IGKV2-24 immunoglobulin...-functional) IGLV233, V1-9 IGLV2-8 immunoglobulin lambda variable 2-8 IGLV28, V1-2 IGLV3-1 immunoglobulin...

...EGFP WPRE LTR H1 shSOD1 SOD1 H1 ALS Patrick Aebischer 10881 pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch... 14542 pLV.ATMi ATM H1 Ataxia telangiectasia Didier Trono 14581 pSUPER.ATMi ATM H1 Ataxia telangiectasia...shSOD1mismatch SOD1 H1 ALS Patrick Aebischer 11442 pBabe bleo human Frataxin FXN Friedreich ataxia Ronald Kahn 11443...Parkinson's Cheryl Arrowsmith 26210 pSUPER TBK1 TBK1 H1 ALS Tom Maniatis 26365 pENTR4_FLAG/HA_FUS_His6 FUS...CaV2.1 (pCR3.1) CACNA1A CMV Spinocerebellar ataxia 8 Diane Lipscombe 26578 CaV2.1 (pcDNA6) CACNA1A His,...David Root 78523 pFUChW Parkin shRNA Mul1 shRNA PRKN H1 Parkinson's David Chan 78622 pX335_HR_Prnp_3 PRNP...