...Plant 8(8) (2015): 1274-1284. PubMed PMID: 25917172. Find plasmids from this paper at Addgene. 8. Xie...downstream of the 7SK, human U6, mouse U6, or human H1 promoters. If you express fewer than four gRNAs, ...Plasmids: These plasmids can be used to express up to 8 gRNAs after Golden Gate or Gibson Assembly. Using ...containing vector using Golden Gate assembly, and up to 8 gRNAs may be expressed simultaneously. Vectors for...

...your target gene! Tanenbaum et al., Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. Multi-bit genetic memory...expression levels. Zhang et al., PLoS One. 2012;7(8):e43072. doi: 10.1371/journal.pone.0043072. Photoswitchable... (U6) expression, shRNA/shRNA-miR30 constitutive (H1, U6, 7SK) or inducible shRNA-miR30 (CMV-TO) expression...Boncompain et al., Nat Methods 2012. Mar 11;9(5):493-8. MXS cloning platform for imaging and flow cytometry...blocks including 14 different fluorescent proteins, 8 constitutive promoters, 2 includible promoters, 3 ...Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. Plasmid tools for all-optical electrophysiology ...