Skip to main content
Addgene
Showing: 1 - 15 of 15 results

  1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...iteration was named GEmCherry1, due to the “genomic fragment inserted into mCherry”. However this one ...AAVS1 locus using GEmCherry2. In this experiment they saw a clear difference in mCherry expression using...like to mutate in the genome) and an out-of-frame H2B (histone)-GFP reporter into the genome of mammalian... puromycin resistance gene linked to an in-frame H2B-GFP. This visual read out allows for easy screening...targeted the gene NFE2L2, the Madison lab found that H2B-GFP expressing cells exhibited a 2-4 fold increase...CRISPR genomic target region into the N-terminus of mCherry. To prevent alternative start site initiation, ...genomic region inserted into the N-terminus of mCherry can be targeted by a sgRNA guided Cas9 causing ...

  2. Hot Plasmids - February 2022

    Type
    Blog Post
    ...4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin), and mitochondrial (mito-mCherry-1xFLAG...while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG frankenbody...Schematic of anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP...

  3. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...-hM3D(Gq)-mCherry AAV1 Chemogenetics Bryan Roth New serotype pAAV-CaMKIIa-hM4D(Gi)-mCherry AAV1 Chemogenetics...pAAV-hSyn-hM3D(Gq)-mCherry AAV1 Chemogenetics Bryan Roth New serotype pAAV-hSyn-hM4D(Gi)-mCherry AAV1 Chemogenetics...: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) AAV-PHPeB Control Jonathan...Control Gordon Fishell New viral prep pAAV_BiSSTe10_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell...Control Gordon Fishell New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell...Control Gordon Fishell New viral prep pAAV_BiVIPe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell ...Control Gordon Fishell New viral prep pAAV_BiLAMP5e3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell...

  5. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Chromatin H2B EGFP Mark Mercola 21217 PGK-H2BmCherry Chromatin H2B mCherry Mark Mercola 20972 H2B-mCherry Chromatin...Chromatin H2B GFP Elaine Fuchs 11680 H2B-GFP Chromatin H2B GFP Geoff Wahl 21210 PGK-H2BeGFP Chromatin ...Chromatin H2B mCherry Robert Benezra 80001 pH2B-miRFP703 Chromatin H2B miRFP703 Vladislav Verkhusha 17650...55165 mCherry-ZO1-C-14 Tight Junctions Zonula Occludens-1 mCherry Michael Davidson 55001 mCherry-Beta-... PI 110060 FUW mCherry-GFP-LC3 Autophagosome LC3 mCherry, GFP Anne Brunet 40827 mCherry-hLC3B-pcDNA3.1...pHIV-H2BmRFP Chromatin H2B mRFP Bryan Welm, Zena Werb 21045 pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry...mGold Francois St-Pierre 27705 CAV1-mCherry Caveolae Cav1 mCherry Ari Helenius 37533 cfSGFP2-N Extracellular...

  6. MXS Chaining

    Type
    Blog Post
    ...localization 1 TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived...the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm...

  7. Retrograde AAV viral preps

    Type
    Collection
    ...pAAV-hSyn-mCherry Syn mCherry Control Deisseroth 114469 pAAV-CaMKIIa-mCherry CamKII mCherry Control Deisseroth...pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent...176748 AAV-Syn-H2B-jGCaMP8m-WPRE Syn H2B-GCaMP8m Calcium sensor Looger 176749 AAV-Syn-H2B-jGCaMP8s-WPRE...Deisseroth 50459 pAAV-hSyn-DIO-mCherry Syn mCherry, Cre-dependent Control Roth 59462 pAAV-CAG-tdTomato...pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses NLS-mCherry in the absence of Cre...Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate mCherry expression Recombinases...Wilson 55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression Recombinases...

  8. Brain Armamentarium

    Type
    Collection
    ...Gradinaru 213945-PHPeB pAAV_BiSSTe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin...Gradinaru 213941-PHPeB pAAV_BiPVe3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin...Gradinaru 213937-PHPeB pAAV_BiPVe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin...Gradinaru 213915-PHPeB pAAV_BiLAMP5e3_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin...Gradinaru 213856-PHPeB pAAV_BiVIPe4_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin...Gradinaru 213830-PHPeB pAAV_BiCHATe27_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin...Gradinaru 213815-PHPeB pAAV_BiSSTe10_ChR2_mCherry AAV construct expressing mCherry-tagged channelrhodopsin...

  9. Caltech Systemic Capsids

    Type
    Collection
    ...pAAV-CaMKIIa-mCherry CamKIIa mCherry Control Deisseroth 114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth...Ting 163509 CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA hSyn1 H2B-SYFP2 Control Ting 191706 AiP13044 - pAAV-AiE0779m...pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor...: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) minBG SYFP2 Control AIBS...Activator C1V1 Dimidschstein 135634 pAAV-S5E2-ChR2-mCherry E2 regulatory element Activator ChR2 Dimidschstein...213815 pAAV_BiSSTe10_ChR2_mCherry BiSSTe10 Activator ChR2 Fishell 213830 pAAV_BiCHATe27_ChR2_mCherry BiCHATe27...213856 pAAV_BiVIPe4_ChR2_mCherry BiVIPe4 Activator ChR2 Fishell 213915 pAAV_BiLAMP5e3_ChR2_mCherry BiLAMP5e3...

  10. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for...

  11. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules mCherry-alpha-tubulin...mito-BFP mCherry-Drp1 (GTPase in division) GFP-Mff (outer membrane protein) Late Endosome mCherry-Rab7A...Pre-constructed Entry vectors containing Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or...one of three fluorescent proteins (GFP, BFP and mCherry), the authors were able to visualize simultaneously...various pieces for inducible expression, loxP sites, H2B, P2A or PEST2D sequences. Several pre-constructed...mCherry-alpha-tubulin Early Endosome Vacuolar Compartment mCherry-Rab5 BFP-Rab5 GFP-Rab5B Vacuolar & Budding Compartment...

  12. Optogenetics AAV Preps

    Type
    Collection
    ...-ChR2-mCherry nEF ChR2 mCherry Cre dependent 8 Deisseroth 137144 pAAV-nEF-Coff/Fon-ChR2-mCherry nEF ChR2...1, 9 Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, 9, rg* Fishell 135634...135634 pAAV-S5E2-ChR2-mCherry nEF ChR2 mCherry Constitutive 1, 9, PHPeB Dimidschstein 137142 pAAV-nEF-...pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2 mCherry Cre and Flp dependent 8 Deisseroth 137143 pAAV-nEF-Con/Foff 2.0...pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a ChR2/H134R mCherry Cre dependent 1, 5, 8, 9, rg*...Deisseroth 26975 pAAV-CaMKIIa-hChR2(H134R)-mCherry CaMKII ChR2/H134R mCherry Constitutive 1, 5, 9, rg* Deisseroth...Deisseroth 26976 pAAV-hSyn-hChR2(H134R)-mCherry Syn ChR2/H134R mCherry Constitutive 5, 8, 9 Deisseroth 28017...

  13. Brain Initiative Collection

    Type
    Collection
    ...Gordon Fishell 83898-AAV1 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic...Gordon Fishell 83898-AAV9 pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic... Fishell 83898-AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 ChR2-mCherry fusion expression in forebrain GABA-ergic...135634-AAV1 pAAV-S5E2-ChR2-mCherry AAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder...135634-AAV9 pAAV-S5E2-ChR2-mCherry AAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder...-PHPeB pAAV-S5E2-ChR2-mCherry AAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder...expression. Bryan Roth 75033-AAV1 pAAV CD68-hM4D(Gi)-mCherry Expression of hM4D(Gi) using the CD68 promoter....

  14. Cre-lox system

    Type
    Collection
    ...hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean Heat-inducible reporter with Cre dependent H2B-RFP expression...paavCAG-iCre iCre CAG AAV Kim 55632 pAAV-Ef1a-mCherry-IRES-Cre Cre and mCherry coexpression EF-1 alpha AAV Deisseroth...84032 pHD066 mCherry-Cre fusion and Venus Elastase Zebrafish Stainier 84033 pHD157 mCherry-Cre fusion and...Mammalian Hirrlinger 107312 AAV-hSyn-mCherry-P2A-Cre-WPRE mCherry and Cre; expressed in neurons hSyn AAV... AAV Yang 107313 AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA mCherry and Cre; expressed in excitatory neurons...beetle) Averof 119868 pGEM-NLS-Cre-2A-mCherry-FKF NLS-Cre, mCherry T7 Bacterial Sauka-Spengler 119971 GAG-CRErec...Mammalian Capecchi 126006 pAAV2-ProC3-Cre/mCherry Cre and mCherry ProC3 AAV Roska 126700 pHAGE2-EF1aL-Cre-IRES-NeoR-W...

  15. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...(E123A)-mCherry Karl Deisseroth AV-1-35512 35512-AAV1 pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry Karl Deisseroth...20297-AAV1 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Optogenetics Karl Deisseroth AV-1-20298P...AV-1-26975 26975-AAV1 pAAV-CaMKIIa-hChR2(H134R)-mCherry Optogenetics Karl Deisseroth AV-1-35503 35503-AAV1...20297-AAV5 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Optogenetics Karl Deisseroth AV-5-20298P...AV-5-26975 26975-AAV5 pAAV-CaMKIIa-hChR2(H134R)-mCherry Optogenetics Karl Deisseroth AV-5-35509 35509-AAV5...20297-AAV9 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Optogenetics Karl Deisseroth AV-9-20298P...AV-9-26975 26975-AAV9 pAAV-CaMKIIa-hChR2(H134R)-mCherry Optogenetics Karl Deisseroth AV-9-35497 35497-AAV9...
Showing: 1 - 15 of 15 results