...corresponding to known cellular signaling pathways as part of our Genetic Pathway Reference Set. These Gateway-compatible...cDNA clones mapping to well-annotated cellular pathways). It wasn’t clear whether gene-agnostic bioassays...The pan cancer mutant collection and the genetic pathway reference collections are described in this paper...PubMed Central PMCID: PMC5386591.  The genetic pathway reference collection is described in this publication...

...achieved in vivo using a bacterial recombination pathway that is present even in common lab cloning strains...strains. This intrinsic bacterial recombination pathway, referred to as recA-independent recombination, ...functions as a bacterial DNA repair mechanism. The pathway is ubiquitous, with successful recombination reported...E. coli strains tested so far. The use of this pathway as a cloning tool was first reported over 25 years...et al., 2016) uses the bacterial recombination pathway to allow any cloning procedure to be performed ...

...ACS Synth Bio. 2015. PubMed PMID: 26479688. RAS Pathway Clone Collection 2.0 Ras proteins are small GTPases...GTPases involved in cell signaling pathways that control many cellular processes such as differentiation...Understanding how molecules in the Ras signaling pathway interact will allow scientists to develop better... has recently released a collection of 360 Ras pathway plasmids to the scientific community. This collection... from a crowd-sourced map of the RAS signaling pathway and includes 180 unique transcripts. Each gene ...as to examine the mechanisms behind autophagic pathways. Rosado, et al. Autophagy. 2008. PubMed PMID:...-throughput tagging projects can be performed. Schwartz, et al. Genetics. 2016. PubMed PMID: 26837755 ...

...resistance gal Mutation in galactose metabolism pathway Cells cannot grow on galactose only gyrA, gyrA96...taken up by cell mcrA, mcrBC Inactivation of pathway that cleaves methylated cytosine DNA Allows for...methylated) DNA mrr, Δ(mcrC-mrr) Inactivation of pathway that cleaves methylated adenine or cytosine DNA...growth ara disruption of arabinose metabolism pathway Inability to utilize arabinose as a carbon source...growth proAB mutation in proline biosynthesis pathway Requires exogenous proline source for growth ...

...used to recreate natural biosynthetic pathways. Biosynthetic pathways containing multiple enzymes could be...improved cell-free system to examine biosynthetic pathways A new tool for enriching ABE-targeted cells The...transcription/translation system to examine biosynthetic pathways  By Gabrielle Clouse Recently, cell-free transcription...

...effectiveness. Analyze Cell Signaling Pathways Known cellular signaling pathways can be investigated in a high-throughput...promoter linked to luciferase. Activation of the pathway will result in luciferase expression and more complex...treatments can be designed to further explore the pathway. Which luciferase and assay have you used? Share...

...important to know that cells possess two major repair pathways – Non-Homologous End Joining (NHEJ) and Homology...Homology Directed Repair (HDR) – and how these pathways work, as this could be relevant when planning your...structure, and dictate the major events of the pathway: Broken ends are recognized by loading of the ...This is a simplified, streamlined version of this pathway and does not consider the missing or damaged nucleotides...

...senses, GPCRs play roles in initiating signaling pathways in inflammation, and neurotransmission. These ...important molecular switches in signal transduction pathways and consist of a trimer of three subunits: alpha...there, how can scientists decipher the signaling pathways from ligand to receptor to G-protein, and finally...Interested in tracking the downstream signaling pathways mediated by GPCRs? The Kruse Lab constructed plasmids...

...AKT, mTor, and ERK pathways so the lab will be studying the effects on these pathways using biosensors ...Terry Hébert: How GNB1 mutations affect signaling pathways G protein-coupled receptors (GPCRs) detect molecules...GNB1 disorder by understanding affected signaling pathways and how they lead to neurological phenotypes observed...

..., chemogenetic receptors hijack cell signaling pathways and can lead to such responses as decreased heart...2014). The system allows you to study signaling pathways and the impacts of neural circuits on behavior...intracellular interactions and still set off the same pathways, giving rise to the same cellular responses. The...depends on what G-protein and, consequently, what pathway the receptor acts through. The DREADD hM3D(Gq),...

...guide RNA and repair template of your choosing (Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR...mutations that was published in a microPublication (Schwartz and Jorgensen, 2018).  These are just some examples...PMID: 17986455. PubMed Central PMCID: PMC2238901. Schwartz, Matthew L., and Erik M. Jorgensen. "SapTrap, ...: 26837755. PubMed Central PMCID: PMC4905529.  Schwartz, M. L., & Jorgensen, E. M. "SapTrap vectors for...

...then transported in vesicles via the secretory pathway to their subcellular or extracellular destination...organelle or involved in intracellular transport pathways, it is possible to map the internal organization...markers for major mammalian organelles and transport pathways. It is best, though not always possible, to use....  CureFFI.org. Cell Biology 04: The Secretory Pathway. 4. Dunn, Kenneth W., Malgorzata M. Kamocka, and...

...pinhead mutant, which regulates a developmental pathway implicated in congenital heart defects and cancer...lineage tracing can help illuminate how signaling pathways—such as those regulated by one-eyed pinhead—contribute...also showed that in the absence of the signaling pathway regulated by one-eyed pinhead, incorrect proportions... zygotes into adults and explain how signaling pathways control this process. Additional zebrafish applications...

...understanding of the Z pathway" is vague and "what proteins regulate the Z pathway?" is an open-ended list...consider "how do proteins X and Y regulate the Z pathway?" A well-defined outcome makes it easier for the...postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health, was an Associate...

...functions, from endosomal trafficking to signaling pathways to the cell cycle. Good tools for measuring intracellular...cell culture and reversible control of signalling pathways. These LIDs are generalizable, versatile and powerful...reversibly switch off and on your favourite signalling pathways (Figure 2). They are all available now at Addgene...Understanding how a protein traffics through these pathways is key to understanding, and potentially perturbing... which can then be tracked along the secretory pathway (Figure B). The RUSH system has already been used...modified sdChr- a blue-shifted channelrhodpson from freshwater green alga identified by Ed Boyden’s lab. The...for identifying previously unknown factors and pathways involved in pre-mRNA splicing in a compound screen...

...biosensors can enable investigation of a signaling pathway or measurement of a biomolecule while largely preserving...calcium signaling in the hippocampal mossy fiber pathway. Neuron. 2014 Apr 16;82(2):413-29. Baljit Khakh...imaging. Front Mol Neurosci. 2013 Sep 24;6:26. John Garthwaite cGMP (cyclic GMP) FRET-based cGMP biosensor SponGee...Cell. 2020 Aug 13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic biosensor measuring...pyruvate A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate carrier...Synaptophysin with tandem repeats of pHluorin Two pathways of synaptic vesicle retrieval revealed by single-vesicle...dynamics. Nature. 2010 Jul 8. 466(7303):263-6. Martin Schwartz Tension Talin tension sensors Talin tension sensor...

...kynurenine pathway of tryptophan degradation. It turns out that activation of the PGC-1a1 pathway increases...protected. Effects of Activation of the PGC-1a1 Pathway Gene expression analysis of the animals’ skeletal...

...Finger Consortium Pathways & ORFs Plasmids are grouped together as part of a pathway or ORF. MAPK Nuclear...Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology Pooled Libraries Pooled...Nuclear Receptors Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific disease...

...approaches. The first route involves identifying a gene/pathway to develop a drug for (aka a druggable target)....reveal individual genes or sometimes even genetic pathways that are worth investigating for drug development...’ proteins – having multiple hits or an entire pathway of possibilities greatly increases the odds of ...

...the Gβγ dimer, activating downstream signaling pathways.  This dissociation can be measured using a technique...system. Conclusions Although the importance of GPCR pathways is well known and, as a result, a target of many...collection can be used to study endogenous ligands, the pathways they activate, as well as the activation by drugs...