...antibodies distributed by Addgene have been produced in-house by Addgene scientists. They are generated using ...Protein band intensities are measured using ImageJ software and a standard curve is generated. The final antibody...calculated from the standard curve. Using ImageJ software, the band intensities of both the antibody and...Each lot of antibody undergoes testing, either in-house or through outside labs, to confirm recognition ...

...Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software and extensive feature...features, such as Cas9, weren’t detected by our in-house software. In fact, SnapGene’s feature library includes...many features as could be detected by our in-house software and also eliminates many of the extraneous ...quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies a variety of common plasmid...Improved Restriction Enzyme Handling - SnapGene’s software positions restriction enzyme annotations throughout...detected is 5x greater than our previous mapping software and now includes Type IIS restriction enzymes ...suggesting these changes, and the SnapGene and Addgene software development teams for working tirelessly to implement...

...Addgene Software Engineer Kris Shamloo wrote a very interesting post about how we develop our in house lab...lab management software. The post is written in terms any programming noob (like myself) can understand...Perfect Science Talk Developing Lab Management Software for Biology Adapting Toehold Switches to Detect...

...Automation and continuous development of in-house software has been crucial for us to keep pace with our...analyze nearly 300 sequences each week. Our internal software was originally designed to help us work in large...collection and analysis eventually outpaced our software, even with continuous updates. The generous donation...scripts or programs, our goal was to find a suitable software tool that could orchestrate the steps into a continuous... continuous pipeline. We also recognized the software system that we chose needed to fulfill certain requirements...,’ in a complete package, all of the pieces of software that depend on each other for the entire package...for convenient compatibility with our internal software infrastructure; however, it is not necessary to...

...genome sequencing Thanks to the establishment of in-house sequencing capabilities and Seqwell’s plexWell technology...The analysis is a 2-step process using Geneious software: First, the individual sequencing reads (~150 ...the unmapped reads, employing our bioinformatics software to assemble the reads into longer DNA contigs ...deeper analysis of the data. Our serotype detection software confirms the serotype of the prep by interrogating...unique to the capsid plasmid. Our recombination software looks for and quantifies the presence of recombinants... post were created using Snapgene and Geneious software. References and resources References Chadeuf G...

...guidelines also provide information on a journal’s house style and the things you need to include in your...GenBank ID) to all antibodies, animal models, and software tools in the Materials and methods section of ...submitting to and make sure your manuscript fits the house style and prerequisites of the journal. Ask colleagues...make sure that your paper fits with the journal’s house style and check that all required information is...

...ready-to-use biosafe Coomassie stain and ImageJ software....(Addgene uses SimplyBlue SafeStain) and ImageJ software. The sample is separated by denaturing polyacrylamide...protein band intensities are measured using ImageJ software and a standard curve is generated. The ratio of...Plastic tray Gel imaging system ImageJ or similar software Reagents Purified recombinant antibody, 0.9–1.1...- can use commercial standard or validate an in-house standard Before Starting Warm the hot plate to 100...Link opens in a new window) or a similar photo software to determine the relative intensity of the protein...

...available gRNA targets in that region. The CRISPR Software Matchmaker can be used to determine the pros and...cons of using many different types of gRNA design software. When you are happy with your selection, ordering...bear in mind that this method will require you to house mice that may have no mutation, costing money and...Repair Additional Resources on Addgene.org Find Software Tools to Help Design You gRNA Find CRISPR Plasmids...

... and our software needs to handle these cases. The following code examples show how software can be structured...Kris Shamloo is a Software Engineer at Addgene and is interested in writing software tools to help scientists... this isn’t so bad, for thousands it’s monumentally difficult without software. Tracking lab procedures... Laboratory management software is not a requisite for a functioning lab, but it is for a scaleable lab... will fail you. The benefits of lab management software aren’t just limited to large volume facilities...you in the right direction when writing your own software. None of this is gospel, but we think it's worth...assume that everything will go perfectly and our software simply follows a linear progression directing ...

...Out How We Think about Developing Lab Management Software    The plasmid's journey begins First, each ... tubes are easily accessible and can be made in house if necessary.   Tips for barcoding and tracking ...important to track the location of the container housing the tubes as it is to track the tubes themselves...

...beta testing, the software aims to be a life science protocol repository. The software can be used with...Addgene Thinks About Developing Lab Management Software    Organizing and sharing your updated protocols...publishers, and small start-ups are turning to software apps to solve our need for quicker, more effective...? Maybe having a dedicated lab-use tablet? The software also makes it easy to annotate protocols, make...management. Though this is a subscription-based software, Labguru does offer free trials and pricing options...catalogs on the site. Like Labguru, Quartzy offers software designed with scientists workflow in mind and ...missed some of your favorite apps. If you know of a software app that I missed, let me know in the comments...

...from CRISPR edit data requires the help of robust software tools. As a consequence, a critical step in the...give a detailed analysis of CRISPR editing. ICE software identifies the percentage of genomes that have...Using the ICE tool Learning how to use the ICE software tool is quick and easy. Simply upload your Sanger...increased flexibility and scalability, the ICE software has two analysis formats: sample by sample analysis...samples simultaneously.  The current version of ICE software can analyze indels that result from single or ...SpCas9. More features will be added to the ICE software tool soon. To request other nucleases or other...experiments than other Sanger sequencing-based software tools and is also faster and easier to use. ICE...

...s customizability. It has a large deck that can house many different types of devices. As long as a device...Blog Apps for Lab Life Developing Lab Management Software for Biology Tips from the Repository Trenches:...

...resources. Rather than developing entire new teams in house to solve every problem (although we do that too)...without us having to invest too much of our internal software development resources into it. At the same time...

...vectors to reach scientists around the world. Our Software Engineering Team (and, added in 2018, our Product...Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades to our inventory...information. Addgene has been successful in helping thousands of sharing scientists from laboratories all over...

...mentioned in the above section, there are gRNA softwares available which score candidate gRNAs for on-target...Cas-OFFinder, CCTop, and many more. While such software typically only considers DNA sequence and doesn....2023.1143157 Resources on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s...experiment involves making a large sgRNA library to thousands of genes and targeting and sequencing millions...

... After all of the droplets have been read, the software outputs an image of the fluorescence amplitude... droplets as the dilutions increase. The ddPCR software uses the ratio of positive to negative droplets...built into the technology. One well (containing thousands of droplets) can be sufficient to capture the ...

...cells by size and signals in the flow cytometry software. Flow sample cells, one at a time, through the...two antibodies to upwards of twenty. Laser and software capacity theoretically allow for roughly fifty...as GAPDH or tubulin (sometimes referred to as housekeeping genes). This allows for relative comparison ...

...Kelly utilizes her firsthand experience in the software industry to inform her work on emerging issues...fused genetic material from intended parents is “rehoused” in a separate, enucleated zygote with healthy...

...in silico using publicly available gRNA design software and synthesized. Pooled gRNAs are then cloned ...Next-generation sequencing - CRISPR libraries contain thousands of gRNA plasmids, discerned only by a unique barcode...