... TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, ...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is... pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors and an envelope... lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver... David Sabatini’s lab is a 3rd generation lentiviral vector that contains a scrambled shRNA. This scrambled...Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC...needed for the production of lentiviral particles. It’s capable of inducible expression of shRNA in target...

...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial constitutive... compound of interest. Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene... of interest into this tetracycline inducible, 3rd generation lentiviral vector backbone from the Shih...experiments. Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively high transduction...how to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408... Viral vector topic page Beginners guide to viral vectors Quick guide to all things lentivirus Resources...Murine Stem Cell Virus (MSCV) genomes whereas lentiviral vectors are derived from the human immunodeficiency...

...Neomycin (G418) Mammalian Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression pLenti...Mammalian pLKO.1 - TRC cloning vector - Lentiviral shRNA expression Tet-pLKO-puro - ...Mammalian pLKO.1 hygro - Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible...Zeo DEST - Tet-inducible lentiviral Gateway destination vector for gene expression...expression pWPXL - Lentiviral expression pBI-MCS-EGFP - Tet-inducible Transient ... Tet-inducible lentiviral shRNA expression pMKO.1 puro - Retroviral shRNA...Gateway destination vector for gene expression (tet inducible) Zeocin/Bleo Mammalian pLenti...

... selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection...21374 for dtomato version. Welm 21916 Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection...versions of this plasmid. Root 44012 pInducer20 3rd Tet-inducible lentivirus for ORF expression, multicistronic...Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA plasmid (pGIPz based) for inducible knockdown,...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...

...Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources...Off PI 21916 Tet-pLKO-neo 3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin... LTR promoters ; see article for lentiviral vectors for tet-inducible gene or miRNA expression rtTA On...Resources: Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections...puromycin selection TetR On Wiederschain 41393 pCW57.1 Lentiviral vector for inducible expression for Gateway... rtTA On Root 11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene or shRNA; see...None On Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression of a miR-shRNA; selection...

...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral backbone, with some...those that allow for conditional (Cre-lox) or inducible (Tet) expression are available. To find plasmids...Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing...

... pBS537 tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre...D_creERt2 Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian...Nxph4-2A-CreERT2 Targeting Vector Cre-ERT2 - Tamoxifen inducible; Targeting vector Mouse Nxph4 Mammalian Zeng...Tamoxifen inducible; Targeting vector for Pax7 Tanaka 111187 pBADZ-HisCre Cre; arabinose inducible. PBAD ...components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre...promoterless vector with several available restriction sites none Mammalian Sauer 11957 pBS595 tet-hCMV-EGFPcre...tet-hCMV-EGFPcre Cre-EGFP fusion Tet inducible Mammalian Sauer 11958 pBS532 promoterless GFPcre promoterless CRE-GFP...

... Leong Ho 102970 pLKO.1P shFXN_1 FXN Friedreich ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich...188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off Full...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...Friedreich ataxia Richard Possemato 102975 pLKO.1P shSOD1 SOD1 ALS Richard Possemato 103913 HTRA2_PDZ_...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410... HA-KIF5A KIF5A HA CMV ALS Rosalind Segal 167294 pLKO.1 puro_shTBK1_3185 TBK1 ALS Agnel Sfeir 169375 pET3d-SF-DCTN1...Romet-Lemonne 92200 pINDUCER20-delta-Syn SNCA Myc TRE2 Parkinson's Huda Zoghbi 92201 pINDUCER20-Tau MAPT Myc ...