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We narrowed to 8 results for: inducible lentiviral vector plko tet on

Showing: 1 - 8 of 8 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ... TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, ...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is... pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors and an envelope... lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver... David Sabatini’s lab is a 3rd generation lentiviral vector that contains a scrambled shRNA. This scrambled...Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC...needed for the production of lentiviral particles. It’s capable of inducible expression of shRNA in target...

  2. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial constitutive... compound of interest. Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene... of interest into this tetracycline inducible, 3rd generation lentiviral vector backbone from the Shih...experiments. Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively high transduction...how to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408... Viral vector topic page Beginners guide to viral vectors Quick guide to all things lentivirus Resources...Murine Stem Cell Virus (MSCV) genomes whereas lentiviral vectors are derived from the human immunodeficiency...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...gen lentiviral vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral...pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible empty backbones on our Tetracycline (Tet) Inducible Expression...Puromycin Mammalian Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...Neomycin (G418) Mammalian, Varies Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression Find yeast transformation...cloning vector - Lentiviral shRNA expression pLJM1-EGFP - 3rd gen lentiviral vector for EGFP fusion; PGK...pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen...

  4. Lentivirus Plasmids

    Type
    Collection
    ... selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection...21374 for dtomato version. Welm 21916 Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection...versions of this plasmid. Root 44012 pInducer20 3rd Tet-inducible lentivirus for ORF expression, multicistronic...Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA plasmid (pGIPz based) for inducible knockdown,...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...selection. TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA with...target. rtTA3 Ptet, miniCMV Howard Gu 44012 pInducer20 Lentiviral Tet-On vector for inducible expression.... for many transactivator or tet-inducible vectors. Tetracycline-inducible Backbones Find backbones or ...neomycin selection. TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier...Campeau 128061 pLVX-Tet3G blasticidin Lentiviral Tet-On vector with CMV promoter Tet-On 3G rtTA Oskar Laur...

  6. Mammalian RNAi Tools

    Type
    Collection
    ...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral backbone, with some...those that allow for conditional (Cre-lox) or inducible (Tet) expression are available. To find plasmids...Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing...

  7. Lentiviral Vector Guide

    Type
    Guide
    ... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector...Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically ranges from... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...Second-generation lentiviral plasmids greatly improved the safety of lentiviral vector production by removing...between second- and third-generation lentiviral plasmids. Lentiviral Vector Production Cloning Cloning your...

  8. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... Leong Ho 102970 pLKO.1P shFXN_1 FXN Friedreich ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich...188572 Tet-off TauRD (P301L/V337M) MAPT Myc TRE Parkinson's, FTD Franz-Ulrich Hartl 188573 Tet-off Full...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...Friedreich ataxia Richard Possemato 102975 pLKO.1P shSOD1 SOD1 ALS Richard Possemato 103913 HTRA2_PDZ_...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410... HA-KIF5A KIF5A HA CMV ALS Rosalind Segal 167294 pLKO.1 puro_shTBK1_3185 TBK1 ALS Agnel Sfeir 169375 pET3d-SF-DCTN1...Romet-Lemonne 92200 pINDUCER20-delta-Syn SNCA Myc TRE2 Parkinson's Huda Zoghbi 92201 pINDUCER20-Tau MAPT Myc ...
Showing: 1 - 8 of 8 results