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Showing: 41 - 60 of 222 results
  1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...available in the Sybody Generation Toolbox plasmid kit: SB_concave, SB_loop, SB_convex. These scaffolds ... This selection step involves using a commercial kit for in-vitro translation of the mRNA libraries. Each...plasmids are available in the Sybody Generation Toolbox kit. Find the Seeger lab's SARS-CoV-2 sybodies! References...
  2. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... tools that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...biological systems” as they predict. The pOSIP plasmid kit can be found at Addgene, so what are you waiting ...constructs, while the Patron Lab MoClo Plant Parts kit contains modules specific for plant transformation... For further reading about Nicola Patron's MoClo kit and her plant synbio research, read the Addgene interview...annealing (SSA) assay. This new Platinum Gate TALEN Kit utilizes a 4-module assembly system, which reduces...additional information on using the Platinum Gate TALEN Kit, please see the Yamamoto lab’s protocol for TALEN...specifically to improve plant transgenesis. TheGreenGate kit available at Addgene includes six individual types...
  3. TALEN Plasmids and Kits

    Type
    Collection
    ...and Kits You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs...constructs with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly...human cells. Veit Hornung Musunuru/Cowan Lab TALEN Kit Kit comprised of 834 plasmids of pre-assembled trimer...Gate TALEN and TAL Effector Kit 2.0. Stephen Ekker Platinum Gate TALEN Kit Platinum TALENs assembled by...List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE...expression. Feng Zhang LIC TAL Effector Assembly Kit Assembly by ligation-independent cloning (LIC). Validated...assembly. Kiran Musunuru and Chad Cowan Ekker Lab TALEN Kit All possible tetramers (264) of RVDs, compatible ...
  4. Zinc Finger Consortium: OPEN Reagents

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    Collection
    ... Kits Zinc Finger Consortium OPEN Reagents Zinc Finger Consortium: OPEN Reagents (Kit # 1000000013...section: "The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For...1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to engineer ZFNs...Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and ...Detailed Information Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering... Joung Nature Protocols paper . How to Cite this Kit These plasmids were created by your colleagues. Please....2009.98. Epub 2009 Sep 17. PubMed PMID 19798082 . Kit Contents The following items will be shipped as stabs...
  5. Plasmid Cloning by PCR

    Type
    Blog Post
    ...of the PCR reaction using a kit, such as the QIAquick PCR Purification Kit. The PCR product is now ready...
  6. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...topoisomerase (2).  These days commercially available TOPO kits provide vectors or cloning arms with overhanging...covalently linked to topoisomerase. Vectors in these kits also often have the topoisomerase site inserted ...
  7. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... the entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great ...great information on ELISA troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter...
  8. Plasmid Collections

    Type
    Collection
    ...Synthetic Biology Tetracycline Worm Expression Kits Kits are groups of plasmids that have been packaged...intended for use as a set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering...Browse Addgene’s curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, fluorescent... . We've gathered our curated plasmid resources, kits, and depositor collections on one page to help you...ORF. MAPK Nuclear Receptors Find a Pathway or ORF Kit Disease Research Find deposited plasmids for specific...
  9. Microbiology Resources

    Type
    Collection
    ...the collection. Return to top Plasmid Kits for Microbiology Kits are sets of plasmids intended for use...Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating... in these model organisms. See below for plasmid kits and other resources relevant to these organisms,...listed here. Addgene Microbiology Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence...subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid kit : Integration of DNA into prokaryotic chromosomes...chromosomes - Endy and Shearwin Labs Ralstonia-GG-Kit : Assembly of Ralstonia solanacearum species complex (Rssc...Yewdell Labs Yarrowia lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription...
  10. Worm Expression Resources

    Type
    Collection
    ...the collection. Return to top Plasmid Kits and Collections Kits are sets of plasmids intended for use ...Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used to generate...regarding this kit can be found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen...Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use ...Plasmids and Resources Plasmid Tools Worm Constructs Kits and Collections Other Resources The nematode Caenorhabditis...and Cre expression plasmids. Large collections or kits are described below and not included in this table...links to protocols, can be found below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein...
  11. TALEN Guide

    Type
    Collection
    ...Gate TALEN kit. In this example, the final array is composed of 18 TAL effector repeats. The kit can be used...Bogdanove TALEN kit came online in the summer of 2011 and has already become the best-selling kit in Addgene...Hospital also recently released a TALEN kit through Addgene. This kit, first described in Nature Biotechnology...to TALEN Technologies You may also like... TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally...” Using these plasmids, which are available as a kit from Addgene , custom arrays consisting of 12-31 ...considerable experience in the DNA targeting field. A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at...Biotechnology early in 2011 and they described their kit for assembling TAL effector arrays in a 2012 issue...
  12. Kit Free RNA Extraction

    Type
    Protocol
    ... Protocols RNA Extraction Without a Kit RNA Extraction Without a Kit You may also like...extraction without a kit. Protocols... like... Kit-Free DNA Purification Agarose Gel Purification Molecular Biology Reference Introduction This...how to extract RNA from tissues or cells without a kit. The general steps of this protocol include homogenization... read this blog post on RNA extraction without a kit. Last Update: April 10, 2020 Equipment Refrigerated...
  13. Synthetic Biology - Overview

    Type
    Collection
    ...PUF Assembly Kit Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae...Lab Logic Gates , BIOFAB Promoter/BCD Kit , and pOSIP Plasmid Kit Gray Lab Maize and Rice Transcription...Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular...
  14. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...AlcA) and pGGC011 (AlcR) from red flame GreenGate kit Steroid-regulated LexA promoter XVE (synthetic...
  15. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ... commercial gel purification kit, such as the QIAquick Gel Extraction Kit . Always follow the manufacturer's...important during the DNA purification step, as many kits cannot handle more than a certain total volume of...
  16. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...done, through a variety of methods or commercial kits.  However, antibodies conjugated to horseradish ...that can be activated to emit light with a simple kit, and is readily available on any number of secondary...
  17. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...little more colorful? CRISPRainbow is available as a kit from Addgene.         References 1. Chen, Baohui...Fluorescent Protein Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags...
  18. Fluorescent Protein Guide

    Type
    Collection
    ...over 100 empty backbones. Fluorescent Protein Kits cpFRET Kit: FRET based biosensors - Pertz Fluorescent ...Protein Fusions with mOX FPs Fluorescent Protein Kits Blog: When is a Monomer not a Monomer? Addgene's...Fusion Construct Set - Hamdoun Browse All Plasmid Kits Community Resources FPbase from Talley Lambert at...
Showing: 41 - 60 of 222 results