...needed, and cleaned up using a standard PCR cleanup kit to remove any template DNA and primers.  After performing...

...  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen...the meeting, in addition to useful plasmids and toolkits available at Addgene.  C. elegans open resources... C. elegans meeting. Here I’ll highlight a few toolkits that we have available at Addgene that were discussed...Jorgensen lab. SapTrap is a reagent toolkit and plasmid assembly line for the creation of CRISPR targeting...Matthew L., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification...

... from the agarose using a commercially available kit or standard protocol. Anneal oligos: The oligos should...

...mammalian cells, mice, and even yeast, though (we have a kit!). Choosing a degron tag Are all tags created equal...

...and stored at -80C. Purification and concentration kits that do not use CsCl are commercially available....

...useful tool to have in your molecular biology tool kit. Consider giving it a try next time you’re screening...

... page links to guides, our CRISPR eBook, plasmid kits, and curated pages categorized by both functionally...

... Addgene's Protocol for RNA Extraction Without A Kit External Resources For more research related resources...

...variety of worm gene promoters C. elegans vector kit - Collection of plasmids from Dr. ...expression in E. coli TALENs Gene targeting TALEN kits - Construct a custom TALEN array ...

...Michael Davidson Collection Find Fluorescent Protein Kits ...

...an excellent add-on to the neuroscientist’s tool-kit for studying the brain and we looked forward to the...

...guaranteed editing with the CRISPR Gene Knockout Kit. Please contact us at [email protected] if you...

...   For even more options, check out the 2in1 Kit for FRET and ratiometric BiFC from Christopher Grefen...modular bimolecular fluorescence complementation toolkit from Benjamin Field’s lab. Special Considerations...

...volume of information: basically, a scientist’s tool kit. I wrote a review article on histone modifers in ...

... our Fluorescent Protein Collection Find Plasmid Kits at Addgene Find Plasmid Cloning Protocols References...

...approach requires no special reagents or purification kits, it is low cost and can be immediately adopted by...

...develop low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To see even... COVID-19 diagnostic enzymes. Plasmids in this toolkit include ones for expressing MMLV reverse transcriptase...

...from tracking plasmids from the moment a deposit kit is sent to a depositor, to streamlining the order...

...with the Nicola Patron, who shared the MoClo plant kit via Addgene. For us, transient leaf transformations...

... (Link opens in a new window) sells DNA Assembly kits and master mixes, including NEBuilder HiFi (Link...