...variation in the Klf4 isoform (KLF4s or KLF4L). Polycistronic cassettes that contained KLF4s had overall diminished...Gateway-Compatible Toolbox Podcast Segment The impact of KLF4 N-terminal variants on iPSC generation Deriving ...expression of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming... diminished KLF4 expression levels; this altered stoichiometry impacted reprogramming and global gene ... monocistronic expression of either variation of Klf4 did not lead to the observed reprogramming discrepancies...available at Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription...patterns. Polycistronic cassettes that contained the KLF4L isoform had more robust initiation of reprogramming...

...expression of the reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation of clinical grade...vectors that encoded six factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT)....Plasmid Addgene ID# Encodes pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL 27080 L-MYC and LIN28 pCXLE-hOCT3/...

...expression of mouse Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates... a cocktail of factors (Oct3/4, Sox2, c-Myc, and Klf4) that are known to maintain pluripotency during ...Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc, and Brd3R from five separate lentiviral plasmids...Doxycycline-inducible expression of human Oct4, Sox2, Klf4, and c-Myc from four separate lentiviral plasmids...vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit and run...lentiviral vector for the expression of human Oct4, Klf4, Sox2, and c-Myc Human Induced Pluripotent Stem ...lentiviral vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative Analyses of Human Reprogramming...

.... When the group targeted the enhancer region of Klf4 and the promoter region of Zfp462, which are over...

...cells simply by expressing four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM factors). As the Yamanaka...

...available plasmids, references, and links. KLF1 KLF2 KLF4 KLF5 KLF6 KLF Plasmids Browse Addgene's collection...

... expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated from mouse...

...overexpression of four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic...

... reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids if you’re interested...

...TCGACTAGCACGCAGTAAattcaactacaatccgTTTGGGCTGCGATTCGGA klf4a TAL3478 & TAL3479 TGTCTCACCTGAAACGCCcctgcatgtctacaggTGCGACCTGCGCTGACCA...

...chemokine-like factor C32, CKLF1, CKLF2, CKLF3, CKLF4, HSPC224, UCK-1 CMA1 chymase 1, mast cell CYH, MCT1...chemokine-like factor C32, CKLF1, CKLF2, CKLF3, CKLF4, HSPC224, UCK-1 CLCF1 cardiotrophin-like cytokine...