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We narrowed to 8 results for: lenticas9 blast

Showing: 1 - 8 of 8 results

  1. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...can be expressed from lentiCas9-Blast. Purpose of LentiCas9-blast LentiCas9-blast is a Cas9 expression... were (drumroll please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based...express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate Cas9-expressing...lentiviruses. This means that if you received lentiCas9-blast lentivirus from Addgene, you could use it in...your own lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing... causes growth rate variability when using lentiCas9-blast to generate Cas9-expressing cells. To ensure...

  2. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...with lentiCas9-blast. A549 cells were transduced (MOI = 37) and selected with 1 µg/mL blasticidin for ... transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1). Some cells were then ...Cas9 expression in A549 cells transduced with lentiCas9-blast. Anti-Cas9 Western blotting was performed on... PMID: 18007609. Addgene Materials Used 1. lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang...then expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed...

  4. Lentiviral Prep Service

    Type
    Collection
    ...Antibiotic Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized...encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding...2A GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding...codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang ...

  5. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...were transduced with lentiCas9-Blast 1 and then selected with 1 μg/mL blasticidin for 9 days. Single cells... generated from A549 cells transduced with lentiCas9-Blast 1 . A549 cells were transduced (MOI = 37) and...control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid... and selected with 1 μg/mL blasticidin for 9 days. Single cells from a stable cell pool were then expanded...expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed...

  6. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...were transduced with lentiCas9-Blast and then selected with 1 µg/mL blasticidin for 9 days. Single cells... by expansion of single cells for 13 days. lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid...mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables selection of a stable cell culture...Appropriate antibiotic for selection (e.g. puromycin, blasticidin) Reagent Preparation DMEM Complete: 10% v/v FBS...

  7. CRISPR References and Information

    Type
    Collection
    ...vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids: pVSVg...

  8. Zhang Lab CRISPR Page

    Type
    Collection
    ...sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene...: lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector encoding...
Showing: 1 - 8 of 8 results