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We narrowed to 11 results for: lentiviral packaging vector pspax2

Showing: 1 - 11 of 11 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid from the Didier...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...It can be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such ... lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver... for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids such as...pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging plasmid from the Trono lab contains the ...pRSV-Rev. pMDLg/pRRE - This is a 3rd generation lentiviral packaging plasmid from the Trono lab that contains...

  2. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...on how to package lentiviral vectors, check out Addgene’s Lentiviral Guide and our lentiviral production...integrase-deficient lentiviral vectors, let’s briefly revisit the basic biology of lentiviral vectors (for a more...check out Addgene's Lentiviral Guide). To produce lentiviral vectors, the lentiviral genome is distributed...further! You can package your own lentiviral vector using an integrase-deficient packaging plasmid available...integrase-deficient lentiviral vectors, how they work, and why you might choose them over integrating lentiviral vectors...contain any of the viral packaging genes, the natural cycle of the lentiviral vector ends here and no viral...of the life cycle of a lentiviral vector with an integrase-deficient viral vector. Created with BioRender.com...

  3. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...those are lentiviral constructs. In 2007 we continued to see requests grow for lentiviral packaging and expression...such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO...expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids from Didier Trono's lab ... Our first popular "kit," Andrew Fire's set of vectors for C. elegans research was our most requested ...-puro for retroviral expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs ...

  4. Viral Production at Addgene

    Type
    Blog Post
    ...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...protocols. Browse our AAV vector collection. Lentivirus Production Lentiviral preparations ("-LV” catalog... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles... 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. Cell...

  5. Lentivirus Plasmids

    Type
    Collection
    ...Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...additional packaging components Trono 63586 psPAX2-D64V 2nd Packaging For making Integrase deficient lentivirus...Addgene lentiviral packaging, envelope, and transfer plasmids Viral...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...plasmid ). This table lists common lentiviral envelope and packaging plasmids that can be used with 2nd... with a plasmid encoding Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be ...

  6. Lentivirus Production

    Type
    Protocol
    ...from a lentiviral vector transfected into 293T cells using a polyethylenimine (PEI) transfection protocol... Timeline Day 0: Seed 293T packaging cells Day 1 (pm): Transfect packaging cells Day 2 (am): 18 h post-transfection...polyethersulfone (PES) filter Transfection grade DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer...OptiPro SFM: Reagent Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3 pmol Envelope Plasmid ( pMD2.G ...Viral Vector Guides Mol Bio Protocols Introduction This protocol can be used to produce lentivirus from...for alternative packaging cell lines or transfection reagents. Once produced, lentivirus can be used for...Considerations Before You Start The health of the packaging cell line is critical for obtaining high viral...

  7. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the...scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral...TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning vector. The vector is 8901 base...Producing Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles...-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A.1 The ...TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing... pLKO derived vectors that may also be useful for your experiment. To see these vectors, visit Addgene...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...then generated lentiviral particles in HEK293T cells with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260...tailored deactivation rates Addgene's own empty lentiviral backbones New recombinant antibodies for Glypicans...fusions for membrane display, they recommend the NGR vectors for most experiments. These new tools promise to... toolkit is the empty plasmid backbone: the DNA vector that contains everything needed to replicate inside...very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here at Addgene. Here's...taken them for a test drive! We validated viral packaging and strong expression after inserting EGFP and...launching later this year!  Find Addgene's own Lentiviral backbone plasmids here! Figure 2: Addgene...

  10. Viral Production

    Type
    Collection
    ...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR... . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced... Viral Vector Packaging Service Viral Production Viral... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core (Link ...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...

  11. CRISPR References and Information

    Type
    Collection
    ...amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ...Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF,...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...at Addgene Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...
Showing: 1 - 11 of 11 results