...work? LentiCRISPR v2 purpose As its name suggests, lentiCRISPR v2 is a lentiviral transfer vector used ...Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at...designed to have increased lentiviral titer as compared to previous lentiviral vectors. It can be used both ...Availability and Use LentiCRISPR v2 is available from Addgene both as a single empty vector and in pooled libraries... the Addgene Website Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries...respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The high titer lentivirus production ...production afforded by lentiCRISPR v2 is key to its efficient use in screening experiments; large amounts of virus...

...)-2A-GFP (PX458). lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...mammalian cells. This vector was the top requested plasmid of 2015. What makes lentiCRISPR v2 great? It produces...It can be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such ... David Sabatini’s lab is a 3rd generation lentiviral vector that contains a scrambled shRNA. This scrambled... pooled library screening experiments. Find lentiCRISPR v2.(If you’ve peeked through the rest of the list...our CRISPR posts Read our viral vector blog posts Read our lentivirus guide Resources on the Addgene ...

... compound of interest. Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene...generation lentiviral vector backbone from the Shih lab. You can find more lentiviral expression vectors (empty...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...experiments. Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively high transduction...ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961... Viral vector topic page Beginners guide to viral vectors Quick guide to all things lentivirus Resources...Murine Stem Cell Virus (MSCV) genomes whereas lentiviral vectors are derived from the human immunodeficiency...

...systems, see our Lentiviral Plasmid FAQs. Lentiviral vectors in the lab Lentiviral vectors are incredibly...current (and future) applications of lentiviral vectors! Lentiviral vector history and components In the early...genome-wide screening.   Lentiviral vectors in the clinic Since lentiviral vectors can deliver a large amount... are also working to make lentiviral vectors safer. Since lentiviral vectors integrate into the genome...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus... dividing and non-dividing cells. Lentiviral vectors are the vector of choice for many CRISPR applications...adeno-associated viral vectors can also target non-dividing cells, lentiviral vectors can carry much more...

...Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector for mammalian genome editing ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-...pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers ...

...Library in lentiCRISPR v2-Blast 1000000173 Knockout Mouse Liu 3rd 10 185,836 Mouse GeCKO v2 1000000052...198,810 CRISPRa-v2 83978 1000000091 Activation Human Weissman 3rd 5 10 104,540 209,080 CRISPRa-v2 83996 1000000093...206,421 CRISPRi-v2 83969 1000000090 Inhibition Human Weissman 3rd 5 10 104,535 209,070 CRISPRi-v2 83987 1000000092...hCRISPRa-v2 subpooled libraries 83980 — 83986 Activation Human Weissman 3rd 5 Varies hCRISPRi-v2 subpooled...mCRISPRa-v2 subpooled libraries 83998 — 84004 Activation Mouse Weissman 3rd 5 Varies mCRISPRi-v2 subpooled...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Clear Filters Name ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson...

... Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector for your...genome engineering from a single lentiviral vector Mammalian Lentiviral expression of Cas9, dCas9, or dCas9...50662 Mammalian/Lentiviral See paper none S. pyogenes Blast Sabatini and Lander lentiCRISPR v2 52961 Mammalian...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes Zhang pL-CRISPR.EFS.GFP 57818 Mammalian/Lentiviral BsmBI yes...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes ccdB Wei pLKO5.sgRNA.EFS.PAC 57825 Mammalian/Lentiviral BsmBI ...

...Familial British dementia Jan Johansson 138347 lentiCRISPR v2-sgDnmt1_1 DNMT1 U6 Hereditary sensory neuropathy...neuropathy type IE Yi Zhang 138348 lentiCRISPR v2-sgDnmt1_2 DNMT1 U6 Hereditary sensory neuropathy type IE...LRRK2 His T7 Parkinson's Suzanne Pfeffer 186025 lentiCRISPR v2-sgCOQ2 COQ2 U6 Multiple system atrophy Boyi...Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter...19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker Jackson 129410...CMV Alzheimer's Johanna Ivaska 154900 pDEST-SorLA-v2 SORL1 Venus CMV Alzheimer's Johanna Ivaska 156022...OPTN GST T7 ALS Sascha Martens 218527 plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA EIF2AK2 U6 VWM disease Kivanc...